AB173828

Biotin Anti-6X His tag® antibody [HIS.H8]

Name: Anti-6X His tag® Biotin antibody [HIS.H8] (ab173828) IgG2b | Abcam
Brand: Abcam Limited
SKU: AB173828

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(2 Publications)

Mouse Monoclonal 6X His tag® antibody - conjugated to Biotin. Suitable for IP, ELISA, WB, ICC/IF and reacts with Tag samples. Cited in 2 publications.

View Alternative Names

6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag, 6x his, 6x-HIS

1 Images

Supplier Data

Western blot - Biotin Anti-6X His tag® antibody [HIS.H8] (AB173828)

Note : Predicted MW is for the His tag alone.

All lanes:

Western blot - Biotin Anti-6X His tag® antibody [HIS.H8] (ab173828) at 1/1000 dilution

Lane 1:

E. coli lysate containing a multi-epitope tagged protein at 0.5 µg

Lane 2:

E. coli lysate containing a multi-epitope tagged protein at 0.25 µg

Lane 3:

E. coli lysate containing a multi-epitope tagged protein at 0.125 µg

Lane 4:

E. coli lysate containing a multi-epitope tagged protein at 0.06 µg

Lane 5:

E. coli lysate containing a multi-epitope tagged protein at 0.03 µg

Lane 6:

E. coli lysate containing a multi-epitope tagged protein at 0.015 µg

Secondary

All lanes:

Streptavidin-HRP at 1/20000 dilution

Predicted band size: 0.8 kDa

true

Unconjugated

Anti-6X His tag® antibody [HIS.H8]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HIS.H8

Isotype

IgG2b

Conjugation

Biotin

Excitation/Emission

Carrier free

Applications

ELISA, IP, ICC/IF, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/500 - 1/2000", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.

Biological function summary

Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.

Pathways

The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.

The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information 6X His tag®

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecules and cells 47:100158 PubMed39577746

2024

An RXLR effector disrupts vesicle trafficking at ER-Golgi interface for Phytophthora capsici pathogenicity.

Applications

Unspecified application

Species

Unspecified reactive species

Jihyun Kim,Jesse Kaleku,Haeun Kim,Minji Kang,Hui Jeong Kang,Jongchan Woo,Hongshi Jin,Seungmee Jung,Cécile Segonzac,Eunsook Park,Doil Choi

Molecular therapy. Methods & clinical development 16:108-125 PubMed31934599

2020

Targeting Antigen to the Surface of EVs Improves the Immunogenicity of Human and Non-human Adenoviral Vaccines in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Carly M Bliss,Andrea J Parsons,Raffael Nachbagauer,Jennifer R Hamilton,Federica Cappuccini,Marta Ulaszewska,Jason P Webber,Aled Clayton,Adrian V S Hill,Lynda Coughlan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-6X His tag® antibody [HIS.H8]

Western blot - Biotin Anti-6X His tag® antibody [HIS.H8] (AB173828)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Applications

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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