AB125057

Biotin Anti-alpha smooth muscle Actin antibody [1A4]

Name: Anti-alpha smooth muscle Actin Biotin antibody [1A4] (ab125057) Mouse monoclonal IgG2a | Abcam
Brand: Abcam Limited
SKU: AB125057
Price: 540 USD
Availability: InStock
Rating: 5 (1 reviews)

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What is this?

(1 Review)

(9 Publications)

Mouse Monoclonal alpha smooth muscle Actin antibody - conjugated to Biotin. Suitable for IHC-P and reacts with Human samples. Cited in 9 publications.

View Alternative Names

ACTSA, ACTVS, GIG46, ACTA2, Alpha-actin-2, Cell growth-inhibiting gene 46 protein

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-alpha smooth muscle Actin antibody [1A4] (AB125057)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-alpha smooth muscle Actin antibody [1A4] (AB125057)

IHC image of ab125057 staining in human breast ductal carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab125057, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

HRP

HRP Anti-alpha smooth muscle Actin antibody [1A4]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-alpha smooth muscle Actin antibody [1A4]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4]
519 FITC

FITC Anti-alpha smooth muscle Actin antibody [1A4]
Unconjugated

Anti-alpha smooth muscle Actin antibody [1A4]
Carrier free

Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1A4

Isotype

IgG2a

Light chain type

kappa

Conjugation

Biotin

Excitation/Emission

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-5 µg/mL", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purity

IgG fraction

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha smooth muscle actin (α-SMA) also known as ACTA2 is an actin isoform with a specific role in the contractile function of smooth muscle cells. The molecular weight of α-SMA is approximately 42 kDa. This protein is expressed widely in vascular smooth muscle cells the peritoneal lining and myofibroblasts. It often serves as a marker for these cell types. The expression of α-SMA is critical for the mechanical attributes of cells contributing to the rigidity and contractility of tissues where it is present.

Biological function summary

Alpha smooth muscle actin aids in maintaining the structural integrity of tissues by forming part of the actin cytoskeleton an important element in cellular support. It exists in high concentration in stress fibers contributing to cellular movements and shape maintenance. These actions are essential in various dynamic cellular processes such as cell migration and adhesion. Alpha smooth muscle actin does not typically form complexes but it associates with other components of the actin cytoskeleton to ensure cell stability and function.

Pathways

Alpha smooth muscle actin functions significantly within the TGF-beta signaling pathway which influences cell proliferation differentiation and apoptosis. It interacts closely with proteins such as myosin to facilitate cellular contractility and motility. Additionally α-SMA plays a part in the RhoA/Rho kinase (ROCK) pathway connecting with regulators of actin filament organization. These pathways are essential for modulation of smooth muscle contraction and actin filament assembly contributing to vascular development and wound healing.

Alpha smooth muscle actin is importantly involved in fibrotic diseases and vascular diseases like arteriosclerosis. Increased expression of α-SMA is often observed in fibrotic tissues contributing to pathogenesis due to excessive extracellular matrix deposition. In the context of vascular disorders overexpression of α-SMA can lead to abnormal vascular remodeling often seen in diseases like hypertension. Interactions with proteins such as fibronectin and collagens facilitate these pathological changes highlighting the importance of α-SMA in disease development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

See full target information ACTA2

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Immunity 56:2325-2341.e15 PubMed37652021

2023

Mural cell-derived chemokines provide a protective niche to safeguard vascular macrophages and limit chronic inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Kami Pekayvaz,Christoph Gold,Parandis Hoseinpour,Anouk Engel,Alejandro Martinez-Navarro,Luke Eivers,Raffaele Coletti,Markus Joppich,Flávio Dionísio,Rainer Kaiser,Lukas Tomas,Aleksandar Janjic,Maximilian Knott,Fitsumbirhan Mehari,Vivien Polewka,Megan Kirschner,Annegret Boda,Leo Nicolai,Heiko Schulz,Anna Titova,Badr Kilani,Michael Lorenz,Günter Fingerle-Rowson,Richard Bucala,Wolfgang Enard,Ralf Zimmer,Christian Weber,Peter Libby,Christian Schulz,Steffen Massberg,Konstantin Stark

Nature communications 12:5674 PubMed34584098

2021

Gut bacteria identified in colorectal cancer patients promote tumourigenesis via butyrate secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Shintaro Okumura,Yusuke Konishi,Megumi Narukawa,Yuki Sugiura,Shin Yoshimoto,Yuriko Arai,Shintaro Sato,Yasuo Yoshida,Shunya Tsuji,Ken Uemura,Masahiro Wakita,Tatsuyuki Matsudaira,Tomonori Matsumoto,Shimpei Kawamoto,Akiko Takahashi,Yoshiro Itatani,Hiroaki Miki,Manabu Takamatsu,Kazutaka Obama,Kengo Takeuchi,Makoto Suematsu,Naoko Ohtani,Yosuke Fukunaga,Masashi Ueno,Yoshiharu Sakai,Satoshi Nagayama,Eiji Hara

Communications biology 4:611 PubMed34021256

2021

Telomere damage promotes vascular smooth muscle cell senescence and immune cell recruitment after vessel injury.

Applications

Unspecified application

Species

Unspecified reactive species

Anna K Uryga,Mandy O J Grootaert,Abel M Garrido,Sebnem Oc,Kirsty Foote,Joel Chappell,Alison Finigan,Francesca Rossiello,Fabrizio d'Adda di Fagagna,Dimitra Aravani,Helle F Jorgensen,Martin R Bennett

The Journal of clinical investigation 130:4845-4857 PubMed32749240

2020

Methylation in pericytes after acute injury promotes chronic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Hsiang Chou,Szu-Yu Pan,Yu-Han Shao,Hong-Mou Shih,Shi-Yao Wei,Chun-Fu Lai,Wen-Chih Chiang,Claudia Schrimpf,Kai-Chien Yang,Liang-Chuan Lai,Yung-Ming Chen,Tzong-Shinn Chu,Shuei-Liong Lin

Arteriosclerosis, thrombosis, and vascular biology 39:1645-1651 PubMed31167564

2019

MARK4 (Microtubule Affinity-Regulating Kinase 4)-Dependent Inflammasome Activation Promotes Atherosclerosis-Brief Report.

Applications

Unspecified application

Species

Unspecified reactive species

Marc Clement,Xiao Chen,Hannah L Chenoweth,Zhongzhao Teng,Sarah Thome,Stephen A Newland,James Harrison,Xian Yu,Alison J Finigan,Ziad Mallat,Xuan Li

Blood advances 3:1003-1010 PubMed30940635

2019

Ocular instillation of vitamin A-coupled liposomes containing HSP47 siRNA ameliorates dry eye syndrome in chronic GVHD.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroyuki Ohigashi,Daigo Hashimoto,Eiko Hayase,Shuichiro Takahashi,Takahide Ara,Tomohiro Yamakawa,Junichi Sugita,Masahiro Onozawa,Masao Nakagawa,Takanori Teshima

Nature communications 9:4567 PubMed30385745

2018

Disease-relevant transcriptional signatures identified in individual smooth muscle cells from healthy mouse vessels.

Applications

Unspecified application

Species

Unspecified reactive species

Lina Dobnikar,Annabel L Taylor,Joel Chappell,Phoebe Oldach,Jennifer L Harman,Erin Oerton,Elaine Dzierzak,Martin R Bennett,Mikhail Spivakov,Helle F Jørgensen

Frontiers in physiology 8:849 PubMed29163195

2017

Members of Bitter Taste Receptor Cluster Are Expressed in the Epithelium of Murine Airways and Other Non-gustatory Tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Shuya Liu,Shun Lu,Rui Xu,Ann Atzberger,Stefan Günther,Nina Wettschureck,Stefan Offermanns

Nature communications 8:15700 PubMed28621310

2017

Single-cell profiling reveals heterogeneity and functional patterning of GPCR expression in the vascular system.

Applications

IHC-Fr

Species

Unspecified reactive species

H Kaur,J Carvalho,M Looso,P Singh,R Chennupati,J Preussner,S Günther,J Albarrán-Juárez,D Tischner,S Classen,S Offermanns,N Wettschureck

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-alpha smooth muscle Actin antibody [1A4]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-alpha smooth muscle Actin antibody [1A4] (AB125057)

Related conjugates and formulations (6)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Product promise