AB222758

Biotin Anti-alpha Tubulin antibody [RM113] - C-terminal - Loading Control

Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Alpha-tubulin 4 antibody - conjugated to Biotin. C-terminal. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human TUBA4A.

View Alternative Names

TUBA1, TUBA4A, Tubulin alpha-4A chain, Alpha-tubulin 1, Testis-specific alpha-tubulin, Tubulin H2-alpha, Tubulin alpha-1 chain

2 Images

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Biotin Anti-alpha Tubulin antibody [RM113] - C-terminal - Loading Control (AB222758)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for alpha Tubulin (red) using ab222758 at a 1/200 dilution in ICC/IF. Nuclei counterstained with DAPI (blue).

Supplier Data

Western blot - Biotin Anti-alpha Tubulin antibody [RM113] - C-terminal - Loading Control (AB222758)

All lanes:

Western blot - Biotin Anti-alpha Tubulin antibody [RM113] - C-terminal - Loading Control (ab222758) at 1/1000 dilution

All lanes:

A431 cell lysate

Predicted band size: 50 kDa

false

Unconjugated

Anti-alpha Tubulin antibody [RM113] - C-terminal - Loading Control

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

RM113

Isotype

IgG

Conjugation

Biotin

Excitation/Emission

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human TUBA4A. The exact immunogen used to generate this antibody is proprietary information.

P68366

Specificity

This antibody reacts with alpha tubulin, including both tubulin alpha-1A chain and tubulin alpha-1B chain.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.09% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha tubulin also known as DM1A is an important component of the structural unit called microtubules. This protein weighs approximately 50 kDa and acts as a building block in the cytoskeleton. Alpha tubulin is expressed in various cell types across many organisms playing a fundamental role in maintaining cell shape and enabling intracellular transport. Researchers often acetylate alpha tubulin to study post-translational modifications and its effects on microtubule dynamics.

Biological function summary

Alpha tubulin combines with beta tubulin to form the tubulin dimer which is the basic unit of microtubule polymerization. These dimers assemble into microtubule filaments integral in numerous cellular processes. As part of the cytoskeleton network alpha tubulin facilitates roles in mitosis and meiosis providing spindle structures necessary for chromosome separation. Its participation in cell signaling pathways is noteworthy as it interacts with motor proteins like kinesins and dyneins to aid cellular activities.

Pathways

Alpha tubulin holds critical roles in microtubule-associated processes within the cytoskeleton and intracellular transport pathways. It interacts with the MAP (microtubule-associated protein) family which modulates microtubule stability and impacts cell division and trafficking. Moreover the microtubule pathway involves proteins like tau which bind to stabilize microtubules and are implicated in cellular dynamics and signaling.

Alpha tubulin is relevant in neurodegenerative diseases such as Alzheimer's disease and cancer. Aberrant modifications or mutations in alpha tubulin or associated proteins like tau can disrupt microtubule stability contributing to pathological conditions. In cancer altered expression of alpha tubulin affects cell division leading to tumor progression and chemoresistance. Researchers study alpha tubulin using techniques such as immunofluorescence with Alexa fluor 555 to understand its role in disease better.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

See full target information TUBA4A

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of inflammation research 18:8447-8475 PubMed40589610

2025

Qimai Feiluoping Decoction Inhibits EndMT to Alleviate Pulmonary Fibrosis by Reducing PI3K/AKT/mTOR Pathway-Mediated the Restoration of Autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Ma,Lu Ding,Xiaoyu Zang,Yingying Yang,Wei Zhang,Xiangyan Li,Daqing Zhao,Zepeng Zhang,Zeyu Wang,Linhua Zhao,Xiaolin Tong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-alpha Tubulin antibody [RM113] - C-terminal - Loading Control

Immunocytochemistry/ Immunofluorescence - Biotin Anti-alpha Tubulin antibody [RM113] - C-terminal - Loading Control (AB222758)

Western blot - Biotin Anti-alpha Tubulin antibody [RM113] - C-terminal - Loading Control (AB222758)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise