AB74696

Biotin Anti-alpha Tubulin antibody [TU-01] - Loading Control

Name: Anti-alpha Tubulin Biotin antibody [TU-01] (ab74696) IgG1 | Abcam
Brand: Abcam Limited
SKU: AB74696
Price: 535 USD
Availability: InStock

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(4 Publications)

Mouse Monoclonal Alpha-tubulin 4 antibody - conjugated to Biotin. Suitable for WB, IHC-P, Flow Cyt and reacts with Human samples. Cited in 4 publications.

View Alternative Names

TUBA1, TUBA4A, Tubulin alpha-4A chain, Alpha-tubulin 1, Testis-specific alpha-tubulin, Tubulin H2-alpha, Tubulin alpha-1 chain

3 Images

Flow Cyt

Supplier Data

Flow Cytometry - Biotin Anti-alpha Tubulin antibody [TU-01] - Loading Control (AB74696)

Separation of human monocytes stained using anti-alpha-tubulin (TU-01) Biotin antibody (concentration in sample 8 µg/ml, Streptavidin APC, red-filled) from monocytes stained using mouse IgG1 isotype control (MOPC-21) Biotin antibody (concentration in sample 8 µg/ml, same as alpha-tubulin Biotin, Streptavidin APC, black-dashed) in flow cytometry analysis (intracellular staining).

Flow Cyt

Supplier Data

Flow Cytometry - Biotin Anti-alpha Tubulin antibody [TU-01] - Loading Control (AB74696)

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-alpha-tubulin (TU-01) Biotin antibody (concentration in sample 8 µg/ml, Streptavidin APC).

Supplier Data

Western blot - Biotin Anti-alpha Tubulin antibody [TU-01] - Loading Control (AB74696)

Western blotting analysis labeling alpha-tubulin using ab74696 on lysates of various cell lines under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 μg/ml of ab74696 followed by IRDye800-conjugated streptavidin. A specific band was detected for alpha-tubulin at approximately 54 kDa.

All lanes:

Western blot - Biotin Anti-alpha Tubulin antibody [TU-01] - Loading Control (ab74696)

Predicted band size: 50 kDa

Observed band size: 54 kDa

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Unconjugated

Anti-alpha Tubulin antibody [TU-01] - Loading Control

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

TU-01

Isotype

IgG1

Conjugation

Biotin

Excitation/Emission

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt, IHC-P

applications

Specificity

ab74696 recognizes the defined epitope (aa 65-97) on the N-terminal structural domain of alpha-tubulin. This epitope is conserved within all species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 g/mL", "WB-species-notes": "reducing conditions.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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Product details

The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha tubulin also known as DM1A is an important component of the structural unit called microtubules. This protein weighs approximately 50 kDa and acts as a building block in the cytoskeleton. Alpha tubulin is expressed in various cell types across many organisms playing a fundamental role in maintaining cell shape and enabling intracellular transport. Researchers often acetylate alpha tubulin to study post-translational modifications and its effects on microtubule dynamics.

Biological function summary

Alpha tubulin combines with beta tubulin to form the tubulin dimer which is the basic unit of microtubule polymerization. These dimers assemble into microtubule filaments integral in numerous cellular processes. As part of the cytoskeleton network alpha tubulin facilitates roles in mitosis and meiosis providing spindle structures necessary for chromosome separation. Its participation in cell signaling pathways is noteworthy as it interacts with motor proteins like kinesins and dyneins to aid cellular activities.

Pathways

Alpha tubulin holds critical roles in microtubule-associated processes within the cytoskeleton and intracellular transport pathways. It interacts with the MAP (microtubule-associated protein) family which modulates microtubule stability and impacts cell division and trafficking. Moreover the microtubule pathway involves proteins like tau which bind to stabilize microtubules and are implicated in cellular dynamics and signaling.

Alpha tubulin is relevant in neurodegenerative diseases such as Alzheimer's disease and cancer. Aberrant modifications or mutations in alpha tubulin or associated proteins like tau can disrupt microtubule stability contributing to pathological conditions. In cancer altered expression of alpha tubulin affects cell division leading to tumor progression and chemoresistance. Researchers study alpha tubulin using techniques such as immunofluorescence with Alexa fluor 555 to understand its role in disease better.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

See full target information TUBA4A

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of biophotonics 13:e2419 PubMed31999066

2020

Multicomposite super-resolution microscopy: Enhanced Airyscan resolution with radial fluctuation and sample expansions.

Applications

Unspecified application

Species

Unspecified reactive species

Baoju Wang,Longfang Yao,Yueyue Jing,Yiyan Fei,Qianming Bai,Lan Mi,Jiong Ma

Journal of biophotonics 11:e201800020 PubMed29655260

2018

Joint tagging assisted fluctuation nanoscopy enables fast high-density super-resolution imaging.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiping Zeng,Jing Ma,Peng Xi,Canhua Xu

Journal of biomedical optics 21:66007 PubMed27281064

2016

Superior performance with sCMOS over EMCCD in super-resolution optical fluctuation imaging.

Applications

Unspecified application

Species

Unspecified reactive species

Xuanze Chen,Zhiping Zeng,Rongqin Li,Boxin Xue,Peng Xi,Yujie Sun

Scientific reports 5:8359 PubMed25665878

2015

Fast super-resolution imaging with ultra-high labeling density achieved by joint tagging super-resolution optical fluctuation imaging.

Applications

IHC-P

Species

Unspecified reactive species

Zhiping Zeng,Xuanze Chen,Hening Wang,Ning Huang,Chunyan Shan,Hao Zhang,Junlin Teng,Peng Xi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-alpha Tubulin antibody [TU-01] - Loading Control

Flow Cytometry - Biotin Anti-alpha Tubulin antibody [TU-01] - Loading Control (AB74696)

Flow Cytometry - Biotin Anti-alpha Tubulin antibody [TU-01] - Loading Control (AB74696)

Western blot - Biotin Anti-alpha Tubulin antibody [TU-01] - Loading Control (AB74696)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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