AB1227

Anti-Biotin antibody

Name: Anti-Biotin antibody (ab1227) | Abcam
Brand: Abcam Limited
SKU: AB1227
Price: 4390 USD
Availability: InStock
Rating: 3 (7 reviews)

(7 Reviews)

(18 Publications)

Rabbit Polyclonal Biotin antibody. Suitable for IP, IHC, ELISA, WB, ICC/IF and reacts with Chemical samples. Cited in 18 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to Biotin.

View Alternative Names

Vitamin B4

1 Images

AbReview5535****

Western blot - Anti-Biotin antibody (AB1227)

All lanes:

Western blot - Anti-Biotin antibody (ab1227) at 1/10000 dilution

All lanes:

50ug biotinylated human Ovarian tissue

Secondary

All lanes:

Streptavidin-HRP

true

Exposure time: 10s

This image is courtesy of an Abreview submitted by Mr John Andersen

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Applications

ICC/IF, IHC, WB, IP, ELISA

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "IHC-species-checked": "guaranteed", "IHC-species-dilution-info": "", "IHC-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "(Coating). For primary: 1/1000 - 1/30,000.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

The antibodies were isolated using affinity chromatography with Lysine-Biotin as the ligand.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.1% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Biotin

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:3550 PubMed37321989

2023

Genome-wide association studies identify OsWRKY53 as a key regulator of salt tolerance in rice.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Yu,Chengsong Zhu,Wei Xuan,Hongzhou An,Yunlu Tian,Baoxiang Wang,Wenchao Chi,Gaoming Chen,Yuwei Ge,Jin Li,Zhaoyang Dai,Yan Liu,Zhiguang Sun,Dayong Xu,Chunming Wang,Jianmin Wan

Antioxidants (Basel, Switzerland) 12: PubMed37107281

2023

Inhibitory Peptide of Soluble Guanylyl Cyclase/Trx1 Interface Blunts the Dual Redox Signaling Functions of the Complex.

Applications

Unspecified application

Species

Unspecified reactive species

Chuanlong Cui,Ping Shu,Tanaz Sadeghian,Waqas Younis,Hong Li,Annie Beuve

eLife 11: PubMed35880862

2022

XAB2 dynamics during DNA damage-dependent transcription inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Lise-Marie Donnio,Elena Cerutti,Charlene Magnani,Damien Neuillet,Pierre-Olivier Mari,Giuseppina Giglia-Mari

Journal of assisted reproduction and genetics 38:3175-3193 PubMed34664184

2021

A novel posttranslational modification of histone, H3 S-sulfhydration, is down-regulated in asthenozoospermic sperm.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Qi,Hongjie Pan,Ning Jiang,Meixin Zhang,Shenfei Sun,Xiaofeng Wan,Fangxi Zhang,Lingling Zhang,Hua Diao,Jian Wang,Runsheng Li

Journal of immunology (Baltimore, Md. : 1950) 206:2420-2429 PubMed33941660

2021

Transglutaminase 2 Regulates Innate Immunity by Modulating the STING/TBK1/IRF3 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Luca Occhigrossi,Federica Rossin,Manuela D'Eletto,Maria Grazia Farrace,Fabiola Ciccosanti,Linda Petrone,Alessandra Sacchi,Roberta Nardacci,Laura Falasca,Franca Del Nonno,Ivana Palucci,Evgeni Smirnov,Nick Barlev,Chiara Agrati,Delia Goletti,Giovanni Delogu,Gian Maria Fimia,Mauro Piacentini

Redox biology 41:101904 PubMed33706169

2021

bFGF alleviates diabetes-associated endothelial impairment by downregulating inflammation via S-nitrosylation pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Gen Chen,Ning An,Weijian Ye,Shuai Huang,Yunjie Chen,Zhicheng Hu,Enzhao Shen,Junjie Zhu,Wenjie Gong,Gaozan Tong,Yu Zhu,Lexuan Fang,Chunyuan Cai,Xiaokun Li,Kwonseop Kim,Litai Jin,Jian Xiao,Weitao Cong

Proceedings of the National Academy of Sciences of the United States of America 117:271-277 PubMed31848246

2019

An R2R3 MYB transcription factor confers brown planthopper resistance by regulating the phenylalanine ammonia-lyase pathway in rice.

Applications

Unspecified application

Species

Unspecified reactive species

Jun He,Yuqiang Liu,Dingyang Yuan,Meijuan Duan,Yanling Liu,Zijie Shen,Chunyan Yang,Zeyu Qiu,Daoming Liu,Peizheng Wen,Jie Huang,Dejia Fan,Shizhuo Xiao,Yeyun Xin,Xianian Chen,Ling Jiang,Haiyang Wang,Longping Yuan,Jianmin Wan

Nature communications 10:2535 PubMed31182712

2019

Rif1 S-acylation mediates DNA double-strand break repair at the inner nuclear membrane.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriele A Fontana,Daniel Hess,Julia K Reinert,Stefano Mattarocci,Benoît Falquet,Dominique Klein,David Shore,Nicolas H Thomä,Ulrich Rass

Aging 11:2003-2019 PubMed30981209

2019

Genistein antagonizes gliadin-induced CFTR malfunction in models of celiac disease.

Applications

Unspecified application

Species

Unspecified reactive species

Speranza Esposito,Valeria Rachela Villella,Eleonora Ferrari,Romina Monzani,Antonella Tosco,Federica Rossin,Manuela D'Eletto,Alice Castaldo,Alessandro Luciani,Marco Silano,Gianni Bona,Gian Luigi Marseglia,Luigina Romani,Mauro Piacentini,Valeria Raia,Guido Kroemer,Luigi Maiuri

Cell 175:1887-1901.e18 PubMed30550787

2018

Asymmetric Expression of LincGET Biases Cell Fate in Two-Cell Mouse Embryos.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaqiang Wang,Leyun Wang,Guihai Feng,Yukai Wang,Yufei Li,Xin Li,Chao Liu,Guanyi Jiao,Cheng Huang,Junchao Shi,Tong Zhou,Qi Chen,Zhonghua Liu,Wei Li,Qi Zhou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Biotin antibody

Western blot - Anti-Biotin antibody (AB1227)

Key facts

Host species

Clonality

Isotype

Carrier free

Applications

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (18)

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