Rabbit Polyclonal Biotin antibody. Suitable for IP, IHC, ELISA, WB, ICC/IF and reacts with Chemical samples. Cited in 18 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to Biotin.
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- Nature communications 14:35502023Genome-wide association studies identify OsWRKY53 as a key regulator of salt tolerance in rice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Yu,Chengsong Zhu,Wei Xuan,Hongzhou An,Yunlu Tian,Baoxiang Wang,Wenchao Chi,Gaoming Chen,Yuwei Ge,Jin Li,Zhaoyang Dai,Yan Liu,Zhiguang Sun,Dayong Xu,Chunming Wang,Jianmin WanPubMed 37321989
- Antioxidants (Basel, Switzerland) 12:2023Inhibitory Peptide of Soluble Guanylyl Cyclase/Trx1 Interface Blunts the Dual Redox Signaling Functions of the Complex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChuanlong Cui,Ping Shu,Tanaz Sadeghian,Waqas Younis,Hong Li,Annie BeuvePubMed 37107281
- eLife 11:2022XAB2 dynamics during DNA damage-dependent transcription inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLise-Marie Donnio,Elena Cerutti,Charlene Magnani,Damien Neuillet,Pierre-Olivier Mari,Giuseppina Giglia-MariPubMed 35880862
- Journal of assisted reproduction and genetics 38:3175-31932021A novel posttranslational modification of histone, H3 S-sulfhydration, is down-regulated in asthenozoospermic sperm.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQi Qi,Hongjie Pan,Ning Jiang,Meixin Zhang,Shenfei Sun,Xiaofeng Wan,Fangxi Zhang,Lingling Zhang,Hua Diao,Jian Wang,Runsheng LiPubMed 34664184
- Journal of immunology (Baltimore, Md. : 1950) 206:2420-24292021Transglutaminase 2 Regulates Innate Immunity by Modulating the STING/TBK1/IRF3 Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuca Occhigrossi,Federica Rossin,Manuela D'Eletto,Maria Grazia Farrace,Fabiola Ciccosanti,Linda Petrone,Alessandra Sacchi,Roberta Nardacci,Laura Falasca,Franca Del Nonno,Ivana Palucci,Evgeni Smirnov,Nick Barlev,Chiara Agrati,Delia Goletti,Giovanni Delogu,Gian Maria Fimia,Mauro PiacentiniPubMed 33941660
- Redox biology 41:1019042021bFGF alleviates diabetes-associated endothelial impairment by downregulating inflammation via S-nitrosylation pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGen Chen,Ning An,Weijian Ye,Shuai Huang,Yunjie Chen,Zhicheng Hu,Enzhao Shen,Junjie Zhu,Wenjie Gong,Gaozan Tong,Yu Zhu,Lexuan Fang,Chunyuan Cai,Xiaokun Li,Kwonseop Kim,Litai Jin,Jian Xiao,Weitao CongPubMed 33706169
- Proceedings of the National Academy of Sciences of the United States of America 117:271-2772019An R2R3 MYB transcription factor confers brown planthopper resistance by regulating the phenylalanine ammonia-lyase pathway in rice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun He,Yuqiang Liu,Dingyang Yuan,Meijuan Duan,Yanling Liu,Zijie Shen,Chunyan Yang,Zeyu Qiu,Daoming Liu,Peizheng Wen,Jie Huang,Dejia Fan,Shizhuo Xiao,Yeyun Xin,Xianian Chen,Ling Jiang,Haiyang Wang,Longping Yuan,Jianmin WanPubMed 31848246
- Nature communications 10:25352019Rif1 S-acylation mediates DNA double-strand break repair at the inner nuclear membrane.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGabriele A Fontana et. al.PubMed 31182712
- Aging 11:2003-20192019Genistein antagonizes gliadin-induced CFTR malfunction in models of celiac disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSperanza Esposito,Valeria Rachela Villella,Eleonora Ferrari,Romina Monzani,Antonella Tosco,Federica Rossin,Manuela D'Eletto,Alice Castaldo,Alessandro Luciani,Marco Silano,Gianni Bona,Gian Luigi Marseglia,Luigina Romani,Mauro Piacentini,Valeria Raia,Guido Kroemer,Luigi MaiuriPubMed 30981209
- Cell 175:1887-1901.e182018Asymmetric Expression of LincGET Biases Cell Fate in Two-Cell Mouse Embryos.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiaqiang Wang,Leyun Wang,Guihai Feng,Yukai Wang,Yufei Li,Xin Li,Chao Liu,Guanyi Jiao,Cheng Huang,Junchao Shi,Tong Zhou,Qi Chen,Zhonghua Liu,Wei Li,Qi ZhouPubMed 30550787
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.1% Sodium azide- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Chemical / Small Molecule corresponding to Biotin.
Reactivity data
Select an application| IP | IHC | ELISA | WB | ICC/IF | |
|---|---|---|---|---|---|
| Chemical | Expected | Expected | Expected | Tested | Expected |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info Use at an assay dependent concentration. | Notes - |
IHC
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info Use at an assay dependent concentration. | Notes - |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info - | Notes (Coating). For primary: 1/1000 - 1/30,000. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
Target data
Alternative names
Vitamin B4
Recommended products
Rabbit Polyclonal Biotin antibody. Suitable for IP, IHC, ELISA, WB, ICC/IF and reacts with Chemical samples. Cited in 18 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to Biotin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.1% Sodium azide- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Chemical / Small Molecule corresponding to Biotin.
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
The antibodies were isolated using affinity chromatography with Lysine-Biotin as the ligand.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
This image is courtesy of a customer review submitted by Mr John AndersenWestern blot - Anti-Biotin antibody (ab1227)
All lanes: Western blot - Anti-Biotin antibody (ab1227) at 1/10000 dilution
All lanes: 50ug biotinylated human Ovarian tissue.
SecondaryAll lanes: Streptavidin-HRP
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10s
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Product protocols
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