AB53494

Anti-Biotin antibody

Name: Anti-biotin antibody - rabbit polyclonal (ab53494) | Abcam
Brand: Abcam Limited
SKU: AB53494
Price: 400 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(74 Publications)

Anti-Biotin antibody (ab53494) is a rabbit polyclonal antibody detecting Biotin in Western Blot, IP, ICC/IF, ELISA.

- Over 50 publications
- Trusted since 2007

View Alternative Names

Vitamin B4

1 Images

AbReview41064****

Immunoprecipitation - Anti-Biotin antibody (AB53494)

RWP1 cells were lysed with Polysomal Lysis
Buffer and diluted in binding buffer (20 mM Hepes
pH 7.6, 150 mM KCl, 3 mM MgCl2, 10% glycerol, 3 mg/ml BSA, 0.2 Triton X-100, 20 μg/ml poly dI-dC, 1 mM DDT, plus protease and RNase inhibitors). Pre-clearing was performed by incubating with protein G-agarose blocked with salmon sperm (1 mg/mL) and mouse IgG (10 μg/mL). After pre-clearing, samples (500 μg) were incubated 4 hours in binding buffer with the DNA-biotinylated probes (2 μg) and antibody against biotin (20 μg/mL) in a final volume of 1 mL. Samples were pulled-down with protein G-agarose, washed with binding buffer supplemented with 0.1%
Tween-20 and analyzed by Western Blot for Suz12.

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Immunoprecipitation - Anti-Biotin antibody (ab53494)

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Image courtesy of an anonymous AbReview

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Applications

WB, IP, ICC/IF, cELISA

applications

Specificity

Reacts with free biotin and biotinylated antibody and proteins

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "cELISA" : {"fullname" : "Competitive ELISA", "shortname":"cELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "20 µg/mL", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "cELISA-species-checked": "guaranteed", "cELISA-species-dilution-info": "", "cELISA-species-notes": "(sensitivity: 0.01 ng/mL)." } } }

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Product details

Anti-Biotin antibody (ab53494) is a rabbit polyclonal antibody and is validated for use in ICC/IF, IP, WB, cELISA in catshark, common marmoset, dogfish, human, mouse, rat samples.

Anti-Biotin antibody (ab53494) has been cited over 56 times in peer reviewed journals and is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-Biotin antibody (ab53494) has high sensitivity and specificity.

Anti-Biotin antibody (ab53494) specifically detects biotin and biotinylated proteins and is sold in 1 mg selling sizes.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Affinity purified with d-Biotin-agarose beads

Storage buffer

pH: 6 - 8 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Biotin

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Publications (74)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40869329

2025

Adaptation of the Protocol for the Isolation of Biotinylated Protein Complexes for Tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Igor A Shokodko,Rustam H Ziganshin,Nadezhda E Vorobyeva

Nature microbiology 10:1463-1483 PubMed40389650

2025

The fast-evolving FIKK kinase family of Plasmodium falciparum can be inhibited by a single compound.

Applications

Unspecified application

Species

Unspecified reactive species

Hugo Belda,David Bradley,Evangelos Christodoulou,Stephanie D Nofal,Malgorzata Broncel,David Jones,Heledd Davies,M Teresa Bertran,Andrew G Purkiss,Roksana W Ogrodowicz,Dhira Joshi,Nicola O'Reilly,Louise Walport,Andrew Powell,David House,Svend Kjaer,Antoine Claessens,Christian R Landry,Moritz Treeck

Functional & integrative genomics 25:103 PubMed40381037

2025

3'UTR RNA editing driven by ADAR1 modulates MDM2 expression in breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Elanur Almeric,Deniz Karagozoglu,Mustafa Cicek,Didem Naz Dioken,Huseyin Avni Tac,Esra Cicek,Busra Aytul Kirim,Irmak Gurcuoglu,Osman Ugur Sezerman,Nurhan Ozlu,Ayse Elif Erson-Bensan

Protein science : a publication of the Protein Society 34:e70140 PubMed40371716

2025

CXXC5 is a ubiquitinated protein and is degraded by the ubiquitin-proteasome pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hazal Ayten,Pelin Toker,Gizem Turan Duman,Çağla Ece Olgun,Öykü Deniz Demiralay,Büşra Bınarcı,Gizem Güpür,Pelin Yaşar,Hesna Begüm Akman,Per Haberkant,Mesut Muyan

Nature communications 15:6667 PubMed39107290

2024

Loss of cold tolerance is conferred by absence of the WRKY34 promoter fragment during tomato evolution.

Applications

Unspecified application

Species

Unspecified reactive species

Mingyue Guo,Fengjun Yang,Lijuan Zhu,Leilei Wang,Zhichao Li,Zhenyu Qi,Vasileios Fotopoulos,Jingquan Yu,Jie Zhou

Clinical cancer research : an official journal of the American Association for Cancer Research 30:4464-4481 PubMed39078310

2024

Pharmacologic Inhibition of EIF4A Blocks NRF2 Synthesis to Prevent Osteosarcoma Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Michael M Lizardo,Christopher Hughes,Yue Z Huang,Taras Shyp,Alberto Delaidelli,Hai-Feng Zhang,Sol Snir Shaool,Annalena F Renner,Farez Burwag,Leanne C Sayles,Alex G Lee,Alejandro Sweet-Cordero,Poul H Sorensen

Genes to cells : devoted to molecular & cellular mechanisms 29:254-269 PubMed38247314

2024

Mouse transient receptor potential melastatin 2 (TRPM2) isoform 7 attenuates full-length mouse TRPM2 activity through reductions in its expression by targeting it to ER-associated degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Shinichiro Yamamoto,Naoto Kiyatake,Akihiro Kaneko,Masanao Shimamura,Takashi Yoshida,Shunichi Shimizu

Protein science : a publication of the Protein Society 33:e4859 PubMed38145972

2023

Dynamic proximity interaction profiling suggests that YPEL2 is involved in cellular stress surveillance.

Applications

Unspecified application

Species

Unspecified reactive species

Gizem Turan,Çağla Ece Olgun,Hazal Ayten,Pelin Toker,Annageldi Ashyralyyev,Büşra Savaş,Ezgi Karaca,Mesut Muyan

Redox biology 69:102986 PubMed38091879

2023

Estrogen genotoxicity causes preferential development of Fuchs endothelial corneal dystrophy in females.

Applications

Unspecified application

Species

Unspecified reactive species

Varun Kumar,Neha Deshpande,Mohit Parekh,Raymond Wong,Shazia Ashraf,Muhammad Zahid,Hanna Hui,Annie Miall,Sylvie Kimpton,Marianne O Price,Francis W Price,Frank J Gonzalez,Eleanor Rogan,Ula V Jurkunas

eLife 12: PubMed37737226

2023

PerTurboID, a targeted in situ method reveals the impact of kinase deletion on its local protein environment in the cytoadhesion complex of malaria-causing parasites.

Applications

Unspecified application

Species

Unspecified reactive species

Heledd Davies,Hugo Belda,Malgorzata Broncel,Jill Dalimot,Moritz Treeck

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Biotin antibody

Immunoprecipitation - Anti-Biotin antibody (AB53494)

Key facts

Host species

Clonality

Isotype

Carrier free

Applications

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (74)

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