Mouse Monoclonal Biotin antibody. Carrier free. Suitable for WB and reacts with Chemical samples. Immunogen corresponding to Chemical / Small Molecule corresponding to Biotin.
Images
![Western blot - Anti-Biotin antibody [B-E24] - BSA and Azide free (AB288529), expandable thumbnail](https://content.abcam.com/products/images/biotin-antibody-b-e24-bsa-and-azide-free-ab288529--western-blot-img179882.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Biotin antibody [B-E24] - BSA and Azide free (AB288529)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.3 - 7.5
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Chemical / Small Molecule corresponding to Biotin.
Reactivity data
Select an application| WB | |
|---|---|
| Chemical | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info - | Notes - |
Target data
Alternative names
Vitamin B4
Recommended products
Mouse Monoclonal Biotin antibody. Carrier free. Suitable for WB and reacts with Chemical samples. Immunogen corresponding to Chemical / Small Molecule corresponding to Biotin.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.3 - 7.5
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Chemical / Small Molecule corresponding to Biotin.
- Carrier free
- Yes
- Clone number
- B-E24
- Purification technique
- Affinity purification Protein A
- Light chain type
- kappa
- Concentration
- Purification notes
Purified from cell culture supernatant
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Hybridoma: Myeloma X63/AG.8653 x Balb/c spleen cells.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-Biotin antibody [B-E24] - BSA and Azide free (ab288529), expandable thumbnail](https://content.abcam.com/products/images/biotin-antibody-b-e24-bsa-and-azide-free-ab288529--western-blot-img179882.jpg "Western blot - Anti-Biotin antibody [B-E24] - BSA and Azide free (ab288529)")
Western blot - Anti-Biotin antibody [B-E24] - BSA and Azide free (ab288529)
MW: molecular weight ladder
Gel: 5-15% SDS PAGE
Deteced by chemiluminescence
Non-reducing conditions
All lanes: Western blot - Anti-Biotin antibody [B-E24] - BSA and Azide free (ab288529) at 1 µg/mL
Lane 1: Unbiotinylated IL-6 at 0.1 µg
Lane 2: Biotinylated IL-6 at 0.1 µg
SecondaryAll lanes: Anti-IgG1 HRP at 1/25000 dilution
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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