AB288529
Anti-Biotin antibody [B-E24] - BSA and Azide free
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Mouse Monoclonal Biotin antibody. Carrier free. Suitable for WB and reacts with Chemical samples. Immunogen corresponding to Chemical / Small Molecule corresponding to Biotin.
1 Images
![Western blot - Anti-Biotin antibody [B-E24] - BSA and Azide free (AB288529)](https://content.abcam.com/products/images/biotin-antibody-b-e24-bsa-and-azide-free-ab288529--western-blot-img179882.jpg/_jcr_content/renditions/webp-768.webp)
- WB
Supplier Data
Western blot - Anti-Biotin antibody [B-E24] - BSA and Azide free (AB288529)
MW : molecular weight ladder
Gel : 5-15% SDS PAGE
Deteced by chemiluminescence
Non-reducing conditions
All lanes:
Western blot - Anti-Biotin antibody [B-E24] - BSA and Azide free (ab288529) at 1 µg/mL
Lane 1:
Unbiotinylated IL-6 at 0.1 µg
Lane 2:
Biotinylated IL-6 at 0.1 µg
Secondary
All lanes:
Anti-IgG1 HRP at 1/25000 dilution
false
Complementary Products
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Biotin antibody [Hyb-8] (AB201341)](https://content.abcam.com/products/images/biotin-antibody-hyb-8-ab201341--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img429305.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Biotin antibody [BTN/2032R] (AB234284)](https://content.abcam.com/products/images/biotin-antibody-btn-2032r-ab234284--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img62731.jpg)
Reactivity data
{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
Product details
Hybridoma: Myeloma X63/AG.8653 x Balb/c spleen cells.
Properties and storage information
Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Purified from cell culture supernatant
Storage buffer
pH: 7.3 - 7.5 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Product protocols
For this product, it's our understanding that no specific protocols are required. You can visit:
- Visit the General protocols
- Visit the Troubleshooting
Target data
Product promise
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