Anti-Biotin antibody [Hyb-8] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Biotin antibody [Hyb-8] - BSA and Azide free (AB234026)

This data was developed using ab201341, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human retina tissue labeling Biotin with ab201341 at 1/2000 dilution followed by LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with biotinylated Anti-CRALBP antibody [EPR23448-119] (incubate for 30 mins at room temperature) followed by ab201341 (incubate for 30 mins at room temperature). Recommend to treat the tissues with Biotin Assay Blocking Kit to remove the endogenous biotin before adding antibodies. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Biotin antibody [Hyb-8] - BSA and Azide free (AB234026)

This data was developed using ab201341, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat retina tissue labeling Biotin with ab201341 at 1/2000 dilution followed by LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with biotinylated Anti-CRALBP antibody [EPR23448-119] (incubate for 30 mins at room temperature) followed by ab201341 (incubate for 30 mins at room temperature) and followed by mouse IgG1 antibody (ab125913, 1 : 1000) for 8 mins. Recommend to treat the tissues with Biotin Assay Blocking Kit to remove the endogenous biotin before adding antibodies. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-Biotin antibody [Hyb-8] - BSA and Azide free (AB234026)

This data was developed using ab201341, the same antibody clone in a different buffer formulation.

Mouse PBMC were blocked with 2.4G2 supernatant at 4°C for 15min, labeled with Rat anti-mouse CD4 conjugated to Biotin at 4°C for 30min, then stained with mouse IgG or ab201341 at 4°C for 30min followed by staining with Goat F(ab')2 Anti-Mouse IgG - Fc (DyLight® 488), pre-adsorbed (ab98736, 1/2000), PE/Cy7 conjugated anti-mouse CD19 Antibody and Alexa Fluor® 647 conjugated anti-mouse CD3 Antibody at 4℃ in the dark. Prior to data acquisition, 7-AAD staining was performed to exclude dead cells.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Biotin antibody [Hyb-8] - BSA and Azide free (AB234026)

This data was developed using ab201341, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse retina tissue labeling Biotin with ab201341 at 1/2000 dilution followed by LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with biotinylated Anti-CRALBP antibody [EPR23448-119] (incubate for 30 mins at room temperature) followed by ab201341 (incubate for 30 mins at room temperature) and followed by mouse IgG1 antibody (ab125913, 1 : 1000) for 8 mins. Recommend to treat the tissues with Biotin Assay Blocking Kit to remove the endogenous biotin before adding antibodies. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-Biotin antibody [Hyb-8] - BSA and Azide free (AB234026)

This data was developed using ab201341, the same antibody clone in a different buffer formulation.

Mouse PBMC were blocked with 2.4G2 supernatant at 4°C for 15min, labeled with Rat anti-mouse CD4 conjugated to Biotin at 4°C for 30min, then stained with mouse IgG or ab201341 at 4°C for 30min followed by staining with Goat F(ab')2 Anti-Mouse IgG - Fc (DyLight® 488), pre-adsorbed (ab98736, 1/2000), PE/Cy7 conjugated anti-mouse CD19 Antibody and Alexa Fluor® 647 conjugated anti-mouse CD3 Antibody at 4℃ in the dark. Prior to data acquisition, 7-AAD staining was performed to exclude dead cells.

• WB

Supplier Data

Western blot - Anti-Biotin antibody [Hyb-8] - BSA and Azide free (AB234026)

This data was developed using ab201341, the same antibody clone in a different buffer formulation.

Western Blot analysis of specific lysates using anti-CRALBP antibody(ab243664) Rabbit mAb (Biotinylated) followed by Anti-biotin (ab201341) Mouse mAb and anti-mouse IgG, HRP linked Antibody ab131368.

Blocking buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Biotin antibody [Hyb-8] (<a href='/en-us/products/primary-antibodies/biotin-antibody-hyb-8-ab201341'>ab201341</a>) at 1/1000 dilution

Lane 1:

Mouse eyeball tissue lysate at 10 µg

Lane 2:

Rat eyeball tissue lysate at 10 µg

Lane 3:

Mouse C57 P20 retina at 10 µg

Secondary

All lanes:

Western blot - Anti-mouse IgG for IP (HRP) (<a href='/en-us/products/secondary-antibodies/mouse-igg-for-ip-hrp-ab131368'>ab131368</a>)

Observed band size: 36 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-Biotin antibody [Hyb-8] - BSA and Azide free (AB234026)

This data was developed using ab201341, the same antibody clone in a different buffer formulation.

Western Blot analysis of specific lysates using anti-Peripherin antibody (ab246502) Rabbit mAb (Biotinylated) followed by Anti-biotin (ab201341) Mouse mAb and anti-mouse IgG, HRP linked Antibody ab131368.

Blocking buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Biotin antibody [Hyb-8] (<a href='/en-us/products/primary-antibodies/biotin-antibody-hyb-8-ab201341'>ab201341</a>) at 1/1000 dilution

Lane 1:

Human nerve lysate at 10 µg

Lane 2:

Human lower limb nerve lysate at 10 µg

Secondary

All lanes:

Western blot - Anti-mouse IgG for IP (HRP) (<a href='/en-us/products/secondary-antibodies/mouse-igg-for-ip-hrp-ab131368'>ab131368</a>) at 1/1000 dilution

Observed band size: 58 kDa

false

Exposure time: 103s