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AB27630

Biotin Anti-Apolipoprotein A I antibody

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(4 Publications)

Goat Polyclonal Apolipoprotein A I antibody - conjugated to Biotin. Suitable for ELISA, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human APOA1.

View Alternative Names

Apolipoprotein A-I, Apo-AI, ApoA-I, Apolipoprotein A1, APOA1

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Reacts with

Human

Applications

ELISA, WB

applications

Immunogen

Native Full Length Protein corresponding to Human APOA1.

P02647

Specificity

This antibody specifically binds to human Apolipoprotein A I in plasma and lipoproteins.

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Alternative Products

Primary Antibodies

AB7613

Anti-Apolipoprotein A I antibody

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Primary Antibodies

AB52945

Anti-Apolipoprotein A I antibody [EP1368Y]

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Primary Antibodies

AB151710

Anti-Apolipoprotein A I antibody [EPSISR27]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein A I antibody [EPSISR27] (AB151710)

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Primary Antibodies

AB185132

Anti-Apolipoprotein A I antibody [EP1368Y] - Low endotoxin, Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein A I antibody [EP1368Y] - Low endotoxin, Azide free (AB185132)

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Primary Antibodies

AB232048

Anti-Apolipoprotein A I antibody [EPSISR27] - BSA and Azide free

RabMAb|Recombinant

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Complementary Products

Assay Kits

AB65390

Cholesterol Assay Kit - HDL and LDL/VLDL

Biochemical assay - Cholesterol Assay Kit - HDL and LDL/VLDL (AB65390)

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Primary Antibodies

AB196194

Alexa Fluor® 647 Anti-Apolipoprotein E antibody [EP1374Y]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Apolipoprotein E antibody [EP1374Y] (AB196194)

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Primary Antibodies

AB139401

Anti-Apolipoprotein B antibody [EPR2914]

RabMAb|Recombinant

Western blot - Anti-Apolipoprotein B antibody [EPR2914] (AB139401)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Assay Kits

AB189576

Human Apolipoprotein AI ELISA Kit

SimpleStep

ELISA - Human Apolipoprotein AI ELISA Kit (AB189576)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This antibody was purified by human Apolipoprotein A I-Sepharose™ affinity column.
Storage buffer
pH: 6.5 - 7.4 Preservative: 0.02% Sodium azide Constituents: 1.23% Sodium phosphate, 0.435% Sodium chloride, 0.01% EDTA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein A-I (ApoA-I) is a major component of high-density lipoprotein (HDL) particles often referred to as 'good cholesterol'. It has a molecular mass of approximately 28 kDa. ApoA-I is mainly expressed in the liver and intestine. It plays a mechanical role in the reverse transport of cholesterol acting to facilitate the efflux of cholesterol from tissues to the liver for excretion. Its alternative names include ApoAI ApoA1 and a component of the AI kits used for measuring this protein.
Biological function summary

ApoA-I functions in cholesterol homeostasis and inflammation. It is a structural component of the HDL complex that mobilizes cholesterol. ApoA-I acts as an activator of the enzyme lecithin-cholesterol acyltransferase (LCAT) which is essential for the maturation of HDL particles. This maturation is necessary for effective cholesterol transport and clearance. ApoA-I's ability to stabilize HDL particles and enhance their functionality makes it significant for maintaining lipid balance and cellular homeostasis.

Pathways

The interaction of ApoA-I with HDL formation and function marks its role in lipid metabolism pathways. Its participation in the reverse cholesterol transport pathway highlights its influence on cardiovascular health. ApoA-I also interacts with other proteins like ApoA-II and paraoxonase-1 which further influence lipid metabolism and antioxidant activities. Understanding these relationships helps elucidate the dynamics of cholesterol removal from the bloodstream.

Disturbances in ApoA-I levels correlate with cardiovascular disease and atherosclerosis. Deficiency or dysfunction in ApoA-I can impair HDL function leading to poor cholesterol removal and buildup within arteries. It is also connected to amyloidosis where misfolded ApoA-I forms deposits in tissues. Understanding these pathological conditions helps researchers target ApoA-I in therapeutic strategies to mitigate disease progression often studying it alongside proteins like ApoB which is associated with low-density lipoprotein (LDL) particles.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
See full target information APOA1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed36613763

2022

The Apparent Organ-Specificity of Amyloidogenic ApoA-I Variants Is Linked to Tissue-Specific Extracellular Matrix Components.

Applications

Unspecified application

Species

Unspecified reactive species

Rita Del Giudice,Mikaela Lindvall,Oktawia Nilsson,Daria Maria Monti,Jens O Lagerstedt

PloS one 16:e0243337 PubMed33826643

2021

Interaction of amphiphilic lipoarabinomannan with host carrier lipoproteins in tuberculosis patients: Implications for blood-based diagnostics.

Applications

Unspecified application

Species

Unspecified reactive species

Shailja Jakhar,Ramamurthy Sakamuri,Dung Vu,Priya Dighe,Loreen R Stromberg,Laura Lilley,Nicolas Hengartner,Basil I Swanson,Emmanuel Moreau,Susan E Dorman,Harshini Mukundan

Arteriosclerosis, thrombosis, and vascular biology 39:1884-1892 PubMed31315438

2019

Association Between ApoA-I (Apolipoprotein A-I) Immune Complexes and Adverse Cardiovascular Events-Brief Report.

Applications

Unspecified application

Species

Unspecified reactive species

David Henson,Ayman Samman Tahhan,David Nardo,Arshed Ali Quyyumi,Vincent J Venditto

Scientific reports 9:6203 PubMed30996333

2019

Direct detection of bacteremia by exploiting host-pathogen interactions of lipoteichoic acid and lipopolysaccharide.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica Z Kubicek-Sutherland,Dung M Vu,Aneesa Noormohamed,Heather M Mendez,Loreen R Stromberg,Christine A Pedersen,Astrid C Hengartner,Katja E Klosterman,Haley A Bridgewater,Vincent Otieno,Qiuying Cheng,Samuel B Anyona,Collins Ouma,Evans Raballah,Douglas J Perkins,Benjamin H McMahon,Harshini Mukundan
View all publications
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Product promise

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