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Rabbit Polyclonal ASPG2 antibody - conjugated to Biotin. Suitable for ELISA, WB and reacts with Escherichia coli samples.


Images

Western blot - Biotin Anti-Asparaginase antibody (AB34616), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

Biotin

Storage buffer

pH: 6.5
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Tripotassium orthophosphate

Form

Liquid

Clonality

Polyclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ELISAWB
Escherichia coli
Expected
Tested

Expected
Expected

Species

Escherichia coli

Dilution info

1/5000 - 1/25000

Notes

-

Tested
Tested

Species

Escherichia coli

Dilution info

1/500 - 1/3000

Notes

-

Targets

ansA, ansA

Alternative names

Recommended products

Rabbit Polyclonal ASPG2 antibody - conjugated to Biotin. Suitable for ELISA, WB and reacts with Escherichia coli samples.

Key facts

Isotype

IgG

Conjugation

Biotin

Form

Liquid

Clonality

Polyclonal

Purification technique

Ion exchange chromatography

Concentration
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Purification notes

Anti-L-Asparaginase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Asparaginase commonly known as L-asparaginase is an enzyme that catalyzes the hydrolysis of L-asparagine into L-aspartate and ammonia. This enzyme is present in various organisms including bacteria such as Escherichia coli. It is often produced using recombinant methods in strains like E. coli as L-asparaginase or trade names like aspergcel. The molecular mass of L-asparaginase is approximately 135 kDa. It is primarily expressed in microbial cells but is also significant in commercial and medical applications for its enzymatic properties.

Biological function summary

This enzyme plays an important role in the metabolism of amino acids. Unlike humans and some tumor cells normal cells can synthesize L-asparagine while certain types of cancer cells lack this ability and depend on external sources. This dependency makes L-asparaginase an effective therapeutic enzyme as it depletes circulating L-asparagine inhibiting protein synthesis in such tumor cells. The enzyme functions independently and does not typically combine into complex structures.

Pathways

L-asparaginase is involved in the asparagine degradation pathway. This pathway impacts protein synthesis and nitrogen metabolism. When applied therapeutically it interacts indirectly with protein synthesis pathways by reducing the availability of asparagine for cells that cannot synthesize it. Additionally when pegylated PEG-asparaginase it displays enhanced stability and a prolonged circulating half-life providing an efficient effect on target pathways.

Associated diseases and disorders

Asparaginase is prominently linked to the treatment of acute lymphoblastic leukemia (ALL). Its mechanism to lower asparagine levels starves leukemia cells of necessary nutrients leading to apoptosis. The enzyme is also studied for its potential effects in certain solid tumors that express deficiencies in asparagine synthetase a related protein. Moreover levels of asparaginase activity are often monitored via specialized assays to ensure therapeutic efficacy and manage side effects during treatment.

Product promise

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1 product image

  • Western blot - Biotin Anti-Asparaginase antibody (ab34616), expandable thumbnail

    Western blot - Biotin Anti-Asparaginase antibody (ab34616)

    All lanes: Western blot - Biotin Anti-Asparaginase antibody (ab34616) at 1/1000 dilution

    All lanes: L-asparaginase at 0.1 µg

    Secondary

    All lanes: HRP-Streptavidin at 1/40000 dilution

    Developed using the ECL technique.

    Predicted band size: 37 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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