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AB34616

Biotin Anti-Asparaginase antibody

4

(1 Review)

|

(1 Publication)

Rabbit Polyclonal ASPG2 antibody - conjugated to Biotin. Suitable for ELISA, WB and reacts with Escherichia coli samples. Cited in 1 publication.

View Alternative Names

b2957, JW2924, ansB, L-asparaginase 2, L-asparaginase II, L-asparagine amidohydrolase II, L-ASNase II

1 Images
Western blot - Biotin Anti-Asparaginase antibody (AB34616)
  • WB

Supplier Data

Western blot - Biotin Anti-Asparaginase antibody (AB34616)

All lanes:

Western blot - Biotin Anti-Asparaginase antibody (ab34616) at 1/1000 dilution

All lanes:

L-asparaginase at 0.1 µg

Secondary

All lanes:

HRP-Streptavidin at 1/40000 dilution

Predicted band size: 37 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Reacts with

Escherichia coli

Applications

ELISA, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Escherichia coli": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/5000 - 1/25000", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
IgG fraction
Purification technique
Ion exchange chromatography
Purification notes
Anti-L-Asparaginase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
Storage buffer
Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Asparaginase commonly known as L-asparaginase is an enzyme that catalyzes the hydrolysis of L-asparagine into L-aspartate and ammonia. This enzyme is present in various organisms including bacteria such as Escherichia coli. It is often produced using recombinant methods in strains like E. coli as L-asparaginase or trade names like aspergcel. The molecular mass of L-asparaginase is approximately 135 kDa. It is primarily expressed in microbial cells but is also significant in commercial and medical applications for its enzymatic properties.
Biological function summary

This enzyme plays an important role in the metabolism of amino acids. Unlike humans and some tumor cells normal cells can synthesize L-asparagine while certain types of cancer cells lack this ability and depend on external sources. This dependency makes L-asparaginase an effective therapeutic enzyme as it depletes circulating L-asparagine inhibiting protein synthesis in such tumor cells. The enzyme functions independently and does not typically combine into complex structures.

Pathways

L-asparaginase is involved in the asparagine degradation pathway. This pathway impacts protein synthesis and nitrogen metabolism. When applied therapeutically it interacts indirectly with protein synthesis pathways by reducing the availability of asparagine for cells that cannot synthesize it. Additionally when pegylated PEG-asparaginase it displays enhanced stability and a prolonged circulating half-life providing an efficient effect on target pathways.

Asparaginase is prominently linked to the treatment of acute lymphoblastic leukemia (ALL). Its mechanism to lower asparagine levels starves leukemia cells of necessary nutrients leading to apoptosis. The enzyme is also studied for its potential effects in certain solid tumors that express deficiencies in asparagine synthetase a related protein. Moreover levels of asparaginase activity are often monitored via specialized assays to ensure therapeutic efficacy and manage side effects during treatment.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information ansB

Additional targets

ansA,ansA
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of experimental medicine 205:2465-72 PubMed18824584

2008

Human PMS2 deficiency is associated with impaired immunoglobulin class switch recombination.

Applications

Unspecified application

Species

Unspecified reactive species

Sophie Péron,Ayse Metin,Pauline Gardès,Marie-Alexandra Alyanakian,Eamonn Sheridan,Christian Peter Kratz,Alain Fischer,Anne Durandy
View all publications
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