Rabbit Polyclonal BDNF antibody - conjugated to Biotin. Suitable for WB, sELISA and reacts with Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human BDNF.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
Biotin
- Storage buffer
Constituents: PBS
- Form
Lyophilized
- Clonality
Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human BDNF. Database link P23560
Reactivity data
Select an application| WB | sELISA | |
|---|---|---|
| Human | Predicted | Predicted |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Cat | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Horse | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Recombinant full length protein - Human | Tested | Tested |
| Rhesus monkey | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info 0.1-0.2 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Cat, Dog, Human, Pig, Chimpanzee, Rhesus monkey, Mouse, Rat, Horse | Dilution info - | Notes - |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info 0.25-1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Cat, Dog, Human, Pig, Chimpanzee, Rhesus monkey, Mouse, Rat, Horse | Dilution info - | Notes - |
Target data
Function
Important signaling molecule that activates signaling cascades downstream of NTRK2 (PubMed:11152678). During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.BDNF precursor formImportant signaling molecule that activates signaling cascades downstream of NTRK2. Activates signaling cascades via the heterodimeric receptor formed by NGFR and SORCS2 (PubMed:24908487, PubMed:29909994). Signaling via NGFR and SORCS2 plays a role in synaptic plasticity and long-term depression (LTD). Binding to NGFR and SORCS2 promotes neuronal apoptosis. Promotes neuronal growth cone collapse (By similarity).
Alternative names
Brain-derived neurotrophic factor, BDNF, Abrineurin, BDNF
Recommended products
Rabbit Polyclonal BDNF antibody - conjugated to Biotin. Suitable for WB, sELISA and reacts with Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human BDNF.
Alternative names
Brain-derived neurotrophic factor, BDNF, Abrineurin, BDNF
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
Biotin
- Storage buffer
Constituents: PBS
- Form
Lyophilized
- Clonality
Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human BDNF. Database link P23560
- Purification technique
Affinity purification
- Reconstitution
Reconstitute in sterile PBS + 0.1% BSA to 0.1-1.0mg/ml.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle, Store in the dark
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Biotin Anti-BDNF antibody (ab245864)
To detect human BDNF by Western Blot analysis ab245864 can be used at a concentration of 0.1 - 0.2 μg/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant human BDNF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Lanes 1-11: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant human BDNF, respectively.
Non-reducing conditions.
All lanes: Western blot - Biotin Anti-BDNF antibody (ab245864)
Predicted band size: 27 kDa
Sandwich ELISA - Biotin Anti-BDNF antibody (ab245864)
To detect humanBDNF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab245864 is required.
This biotinylated polyclonal antibody, in conjunction with a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant human BDNF.
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