AB6645

Biotin Anti-beta Galactosidase antibody

Name: Anti-beta Galactosidase Biotin antibody (ab6645) IgG | Abcam
Brand: Abcam Limited
SKU: AB6645
Price: 515 USD
Availability: InStock

Be the first to review this product! Submit a review

(4 Publications)

Rabbit Polyclonal GLB1/Beta-galactosidase antibody - conjugated to Biotin. Suitable for ELISA, IHC-P, WB, Dot and reacts with Escherichia coli, Recombinant full length protein - Escherichia coli samples. Cited in 4 publications.

View Alternative Names

b0344, JW0335, lacZ, Beta-galactosidase, Beta-gal, Lactase

1 Images

Supplier Data

Western blot - Biotin Anti-beta Galactosidase antibody (AB6645)

All lanes:

Western blot - Biotin Anti-beta Galactosidase antibody (ab6645) at 1/1000 dilution

All lanes:

Beta Galactosidase at 0.05 µg

Secondary

All lanes:

HRP streptavidin conjugate at 1/40000 dilution

Predicted band size: 116 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Excitation/Emission

Carrier free

Reacts with

Escherichia coli

Applications

WB, ELISA, Dot, IHC-P

applications

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Escherichia coli": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/10000 - 1/50000", "ELISA-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/1000 - 1/5000", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Recombinant full length protein - Escherichia coli": { "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "" } } }

style

left-column

Product details

Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per rabbit IgG molecule.

style

left-column

Properties and storage information

Form

Liquid

Purity

IgG fraction

Purification technique

Ion exchange chromatography

Purification notes

Anti-BETA GALACTOSIDASE Antibody, Biotin Conjugated, is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta Galactosidase often called "β-gal" "b-galactosidase" or "b-gal" serves as an important enzyme that hydrolyzes β-galactosides into monosaccharides. It breaks down lactose into glucose and galactose which are easier to absorb. Beta-galactosidase expresses in many organisms such as E. coli where it is a well-known component of the lac operon. Its molecular weight in E. coli is approximately 116 kDa. The enzyme also appears in human tissues but is synthesized and regulated differently compared to bacterial systems.

Biological function summary

This enzyme is involved in the metabolism of galactose a critical aspect of cellular energy production. It can participate in various metabolic pathways converting complex carbohydrates into simpler sugars. In bacteria beta-galactosidase belongs to a protein complex controlled by the lac operon regulating lactose metabolism. In humans the enzyme takes part in lysosomal functions contributing to the degradation of glycoproteins and glycolipids therefore supporting normal cellular processes.

Pathways

Beta-galactosidase plays an important role in the lactose metabolism pathway. In E. coli it coheres with the enzymes of the lac system like lactose permease and transacetylase to facilitate lactose utilization. In human biology it participates in the lysosomal degradation pathway. Its function is associated with proteins such as galactosylceramidase which also break down galactolipids demonstrating its interaction in broader metabolic networks.

Deficiencies or malfunctions in beta-galactosidase lead to metabolic conditions like Galactosialidosis and Morquio syndrome Type B. These are lysosomal storage disorders caused by a lack of enzyme activity leading to accumulation of partially degraded macromolecules. The enzyme's impaired function connects with other proteins such as protective protein cathepsin A in the case of Galactosialidosis causing complex clinical symptoms through disrupted protein interactions and pathways.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

See full target information lacZ

style

left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 7:12136 PubMed28939819

2017

In vivo target exploration of apidaecin based on Acquired Resistance induced by Gene Overexpression (ARGO assay).

Applications

Unspecified application

Species

Unspecified reactive species

Ken'ichiro Matsumoto,Kurato Yamazaki,Shun Kawakami,Daichi Miyoshi,Toshihiko Ooi,Shigeki Hashimoto,Seiichi Taguchi

Journal of neurotrauma 27:2067-76 PubMed20799882

2010

Sustained expression of vascular endothelial growth factor and angiopoietin-1 improves blood-spinal cord barrier integrity and functional recovery after spinal cord injury.

Applications

IHC-Fr

Species

Rat

Juan J Herrera,Laura M Sundberg,Lorena Zentilin,Mauro Giacca,Ponnada A Narayana

The Journal of comparative neurology 518:4675-701 PubMed20963823

2010

Target selection of proprioceptive and motor axon synapses on neonatal V1-derived Ia inhibitory interneurons and Renshaw cells.

Applications

Unspecified application

Species

Unspecified reactive species

Valerie C Siembab,Courtney A Smith,Laskaro Zagoraiou,Maria C Berrocal,George Z Mentis,Francisco J Alvarez

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 19:112-4 PubMed15486057

2004

Generation of purified oligodendrocyte progenitors from embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tamara Glaser,Alberto Perez-Bouza,Katja Klein,Oliver Brüstle

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-beta Galactosidase antibody

Western blot - Biotin Anti-beta Galactosidase antibody (AB6645)

Key facts

Host species

Clonality

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Reactivity data

Product details

Properties and storage information

Form

Purity

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise