Biotin Anti-BrdU antibody
5
(6 Reviews)
|
(56 Publications)
- ICC/IF
AbReview6869****
Immunocytochemistry/ Immunofluorescence - Biotin Anti-BrdU antibody (AB2284)
ab2284 at 1/250 staining primary E12 mouse cortex cells by ICC/IF. The cells were paraformaldehyde fixed, blocked with serum and then incubated with the antibody for 24 hours. Streptavidin conjugated to Alexa-Fluor ® 488 was used as the secondary. The image shows BrdU staining with nuclei counterstained with DAPI.
This image is courtesy of an anonymous Abreview
- IHC-P
AbReview6022****
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-BrdU antibody (AB2284)
ab2284 at 1/2000 dilution staining mouse free floating brain slices by Immunohistochemistry (Formalin/PFA fixed sections). The mice were treated with 100mg/kg BrdU 2 hours before fixation. Free floating 40µm vibratome sections were obtained from paraformaldehyde fixed brains, these were incubated with the antibody for 24 hours. A streptavidin-HRP complex and DAB were used for detection. The image depicts the subventricular zone.
This image is courtesy of an Abreview submitted by Dr Christoph Schwarzer
- IHC-P
AbReview43317****
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-BrdU antibody (AB2284)
ab2284 staining BrdU in mouse intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% H2O2 in methanol for 12 minutes; antigen retrieval was by heat mediation in 10mM citrate, pH6. Samples were incubated with primary antibody (1/100) for 24 hours at 4°C.
This image is courtesy of an anonymous Abreview
- IHC-Fr
AbReview53213****
Immunohistochemistry (Frozen sections) - Biotin Anti-BrdU antibody (AB2284)
ab2284 staining BrdU in Mouse skin tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 10% serum for 30 minutes. Samples were incubated with primary antibody (1/50 in PBS) for 12 hours at 4°C. A Streptavidin Alexa Fluor® 488-conjugated Goat polyclonal (1/500) was used as the secondary antibody.
This image is courtesy of an Abreview submitted by Ahmar Aziz
Reactivity data
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Publications (56)
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American journal of cancer research 14:5230-5250 PubMed39659932
2024
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Journal of Cancer 15:6135-6147 PubMed39440055
2024
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Neural regeneration research 18:1976-1982 PubMed36926722
2023
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Matrix biology plus 16:100125 PubMed36452176
2022
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Cancer discovery 12:2774-2799 PubMed36165893
2022
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Cellular and molecular gastroenterology and hepatology 14:789-811 PubMed35809803
2022
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Arthritis research & therapy 24:6 PubMed34980245
2022
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Bioengineered 12:11823-11836 PubMed34719333
2021
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Development (Cambridge, England) 148: PubMed33462116
2021
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Life science alliance 4: PubMed33536239
2021
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