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AB2284

Biotin Anti-BrdU antibody

5

(6 Reviews)

|

(56 Publications)

Sheep Polyclonal BrdU antibody - conjugated to Biotin. Carrier free. Suitable for ELISA, IHC-FoFr, IHC-P, ICC/IF, IHC-Fr and reacts with Chemical samples. Cited in 56 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to BrdU.
4 Images
Immunocytochemistry/ Immunofluorescence - Biotin Anti-BrdU antibody (AB2284)
  • ICC/IF

AbReview6869****

Immunocytochemistry/ Immunofluorescence - Biotin Anti-BrdU antibody (AB2284)

ab2284 at 1/250 staining primary E12 mouse cortex cells by ICC/IF. The cells were paraformaldehyde fixed, blocked with serum and then incubated with the antibody for 24 hours. Streptavidin conjugated to Alexa-Fluor ® 488 was used as the secondary. The image shows BrdU staining with nuclei counterstained with DAPI.

This image is courtesy of an anonymous Abreview

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-BrdU antibody (AB2284)
  • IHC-P

AbReview6022****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-BrdU antibody (AB2284)

ab2284 at 1/2000 dilution staining mouse free floating brain slices by Immunohistochemistry (Formalin/PFA fixed sections). The mice were treated with 100mg/kg BrdU 2 hours before fixation. Free floating 40µm vibratome sections were obtained from paraformaldehyde fixed brains, these were incubated with the antibody for 24 hours. A streptavidin-HRP complex and DAB were used for detection. The image depicts the subventricular zone.

This image is courtesy of an Abreview submitted by Dr Christoph Schwarzer

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-BrdU antibody (AB2284)
  • IHC-P

AbReview43317****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-BrdU antibody (AB2284)

ab2284 staining BrdU in mouse intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% H2O2 in methanol for 12 minutes; antigen retrieval was by heat mediation in 10mM citrate, pH6. Samples were incubated with primary antibody (1/100) for 24 hours at 4°C.

This image is courtesy of an anonymous Abreview

Immunohistochemistry (Frozen sections) - Biotin Anti-BrdU antibody (AB2284)
  • IHC-Fr

AbReview53213****

Immunohistochemistry (Frozen sections) - Biotin Anti-BrdU antibody (AB2284)

ab2284 staining BrdU in Mouse skin tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 10% serum for 30 minutes. Samples were incubated with primary antibody (1/50 in PBS) for 12 hours at 4°C. A Streptavidin Alexa Fluor® 488-conjugated Goat polyclonal (1/500) was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Ahmar Aziz

Key facts

Host species

Sheep

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Excitation/Emission
Carrier free

Yes

Applications

IHC-FoFr, ICC/IF, IHC-Fr, ELISA, IHC-P

applications

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "IHCFoFr" : {"fullname" : "Immunohistochemistry (PFA perfusion fixed frozen sections)", "shortname":"IHC-FoFr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p>dilute from 1/500 to 1/40,000 against 1mg/mL BrdU analyte.</p>", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "<p>1/2000 (see Abreview).</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p>Fixation in cold methanol for 30 minutes followed by immersion in 7 x 10<sup>-3</sup> N NaOH for 10-15 seconds allows BrdU staining with the simultaneous detection of nuclear cytoplasmic and membrane assigns as well as preservation of morphological detail.</p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.5 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Publications (56)

Recent publications for all applications. Explore the full list and refine your search

American journal of cancer research 14:5230-5250 PubMed39659932

2024

Integration of bioinformatics analysis reveals ZNF248 as a potential prognostic and immunotherapeutic biomarker for LIHC: machine learning and experimental evidence.

Applications

Unspecified application

Species

Unspecified reactive species

Lifang Weng,Zhicheng Cheng,Zhisong Qiu,Jin Shi,Libin Chen,Chunsheng He,Lijuan Wang,Feng Jin

Journal of Cancer 15:6135-6147 PubMed39440055

2024

LINC01134 Directly Binds and Regulates SLC1A5 Stability to Promotes Colorectal Cancer Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Li Yao,Jinxiu Wu,Xiaofeng Wang,Nailing Wang

Neural regeneration research 18:1976-1982 PubMed36926722

2023

C-X-C chemokine receptor type 7 antibody enhances neural plasticity after ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Qian Zhang,Xiao-Yin Wang,Bing-Chao Dong,Mei-Xuan Li,Yu Wang,Ting Xiao,Shan-Shan Zhao

Matrix biology plus 16:100125 PubMed36452176

2022

Modelling stromal compartments to recapitulate the ameloblastoma tumour microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Deniz Bakkalci,Amir Zaki Abdullah Zubir,Syed Ali Khurram,Judith Pape,Kristiina Heikinheimo,Stefano Fedele,Umber Cheema

Cancer discovery 12:2774-2799 PubMed36165893

2022

Stromal Reprogramming by FAK Inhibition Overcomes Radiation Resistance to Allow for Immune Priming and Response to Checkpoint Blockade.

Applications

Unspecified application

Species

Unspecified reactive species

Varintra E Lander,Jad I Belle,Natalie L Kingston,John M Herndon,Graham D Hogg,Xiuting Liu,Liang-I Kang,Brett L Knolhoff,Savannah J Bogner,John M Baer,Chong Zuo,Nicholas C Borcherding,Daniel P Lander,Cedric Mpoy,Jalen Scott,Michael Zahner,Buck E Rogers,Julie K Schwarz,Hyun Kim,David G DeNardo

Cellular and molecular gastroenterology and hepatology 14:789-811 PubMed35809803

2022

MUC1-mediated Macrophage Activation Promotes Colitis-associated Colorectal Cancer via Activating the Interleukin-6/ Signal Transducer and Activator of Transcription 3 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yong H Sheng,Julie M Davies,Ran Wang,Kuan Yau Wong,Rabina Giri,Yuanhao Yang,Jakob Begun,Timothy H Florin,Sumaira Z Hasnain,Michael A McGuckin

Arthritis research & therapy 24:6 PubMed34980245

2022

Hydroxychloroquine administration exacerbates acute kidney injury complicated by lupus nephritis.

Applications

Unspecified application

Species

Unspecified reactive species

Ning An,Chen Yang,Hong-Luan Wu,Yun Guo,Xi-Jie Huang,Tong-Sheng Huang,Zhi-Hong Wu,Jing Xue,Rui-Hong Chen,Zhi-Hang Li,Qing-Jun Pan,Hua-Feng Liu

Bioengineered 12:11823-11836 PubMed34719333

2021

Effect and mechanism of BDNF/TrkB signaling on vestibular compensation.

Applications

Unspecified application

Species

Unspecified reactive species

Dehong Mao,Zhongmei He,Wei Xuan,Jiao Deng,Weichun Li,Xiaoying Fang,Linglong Li,Feng Zhang

Development (Cambridge, England) 148: PubMed33462116

2021

SMAD2 promotes myogenin expression and terminal myogenic differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Émilie Lamarche,Hamood AlSudais,Rashida Rajgara,Dechen Fu,Saadeddine Omaiche,Nadine Wiper-Bergeron

Life science alliance 4: PubMed33536239

2021

Lipocalin 13 enhances insulin secretion but is dispensable for systemic metabolic control.

Applications

Unspecified application

Species

Unspecified reactive species

Lea Bühler,Adriano Maida,Elena Sophie Vogl,Anastasia Georgiadi,Andrea Takacs,Oliver Kluth,Annette Schürmann,Annette Feuchtinger,Christine von Toerne,Foivos-Filippos Tsokanos,Katarina Klepac,Gretchen Wolff,Minako Sakurai,Bilgen Ekim Üstünel,Peter Nawroth,Stephan Herzig
View all publications

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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