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AB25022

Biotin Anti-c-Kit antibody [2B8]

4

(4 Reviews)

|

(18 Publications)

Rat Monoclonal c-Kit antibody - conjugated to Biotin. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 18 publications.

View Alternative Names

CD117, Sl, Kit, Mast/stem cell growth factor receptor Kit, SCFR, Proto-oncogene c-Kit, Tyrosine-protein kinase Kit

1 Images
Flow Cytometry - Biotin Anti-c-Kit antibody [2B8] (AB25022)
  • Flow Cyt

Supplier Data

Flow Cytometry - Biotin Anti-c-Kit antibody [2B8] (AB25022)

Flow Cytometry analysis of C57BL/6 mouse bone marrow cells labeling c-Kit with ab25022 at 0.3 μg/106 cells dilution. Rat Anti-Mouse CD45R-PE followed by Streptavidin-FITC was used as the secondary antibody.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

2B8

Isotype

IgG2b

Light chain type

kappa

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/biotin-rat-igg2b-kappa-monoclonal-rtk4530-isotype-control-ab18542'>ab18542</a> - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

This product was changed from ascites to tissue culture supernatant on 3rd September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified from TCS
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The c-Kit protein also known as CD117 is a type of receptor tyrosine kinase with a molecular weight of approximately 145 kDa. It is encoded by the KIT gene and commonly expressed on the surface of hematopoietic stem cells mast cells melanocytes and germ cells. C-Kit plays a role in the signal transduction pathways that control cell proliferation and survival. Researchers often identify c-Kit using markers like biotin c-Kit PerCP Cy5.5 or antibodies such as anti-c-Kit for experimental purposes.
Biological function summary

C-Kit influences the function and survival of certain cell types. When it binds to its ligand stem cell factor (SCF) it activates receptor dimerization and phosphorylation triggering downstream signaling. C-Kit does not act alone; it often functions in a complex with other proteins to affect cellular outcomes. Its expression is vital for the maintenance of bone marrow hematopoietic cells and contributes to melanocyte and germ cell development.

Pathways

C-Kit interacts with the PI3K/AKT and RAS/ERK pathways through its kinase activity. These pathways play important roles in cell division differentiation and survival. The c-Abl and ACK2 proteins are closely related to c-Kit function within these signaling networks contributing to regulating cellular responses to external stimuli.

C-Kit mutations are frequently associated with gastrointestinal stromal tumors (GIST) and acute myeloid leukemia (AML). These conditions often involve alterations in c-Kit activity leading to uncontrolled cell growth. While studying GIST researchers have noticed interactions between c-Kit and other proteins like CBL which is involved in the modulation of c-Kit signaling. Understanding these interactions helps in designing targeted therapies for conditions involving c-Kit dysregulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.
See full target information Kit
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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Acta neuropathologica communications 9:136 PubMed34389060

2021

The pathogenic role of c-Kit+ mast cells in the spinal motor neuron-vascular niche in ALS.

Applications

Unspecified application

Species

Unspecified reactive species

Mariángeles Kovacs,Catalina Alamón,Cecilia Maciel,Valentina Varela,Sofía Ibarburu,Lucas Tarragó,Peter H King,Ying Si,Yuri Kwon,Olivier Hermine,Luis Barbeito,Emiliano Trias

Scientific reports 11:11603 PubMed34079011

2021

Setd4 controlled quiescent c-Kit cells contribute to cardiac neovascularization of capillaries beyond activation.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng Xing,Jin-Ze Tian,Shu-Hua Yang,Xue-Ting Huang,Yan-Fu Ding,Qian-Yun Lu,Jin-Shu Yang,Wei-Jun Yang

Experimental and therapeutic medicine 22:823 PubMed34131446

2021

Changes in the interstitial cells of Cajal in the gallbladder of guinea pigs fed a lithogenic diet.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Huang,Chao Ding,Xinmin Si

Cell proliferation 54:e13041 PubMed33942933

2021

Therapeutic effects of CXCR4 subpopulation of transgene-free induced cardiosphere-derived cells on experimental myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Jianyong Xu,Huimei Wu,Zhigang Mai,Junbo Yi,Xianqi Wang,Lingyun Li,Zhong Huang

International journal of oncology 57:249-263 PubMed32319605

2020

ADAM17-regulated CX3CL1 expression produced by bone marrow endothelial cells promotes spinal metastasis from hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Chi Sun,Annan Hu,Shengxing Wang,Bo Tian,Libo Jiang,Yun Liang,Houlei Wang,Jian Dong

Glia 68:1165-1181 PubMed31859421

2019

Schwann cells orchestrate peripheral nerve inflammation through the expression of CSF1, IL-34, and SCF in amyotrophic lateral sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Emiliano Trias,Mariángeles Kovacs,Peter H King,Ying Si,Yuri Kwon,Valentina Varela,Sofía Ibarburu,Ivan C Moura,Olivier Hermine,Joseph S Beckman,Luis Barbeito

Brain : a journal of neurology 143:266-288 PubMed31848580

2019

Microglial depletion prevents extracellular matrix changes and striatal volume reduction in a model of Huntington's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Joshua D Crapser,Joseph Ochaba,Neelakshi Soni,Jack C Reidling,Leslie M Thompson,Kim N Green

Experimental hematology 78:21-34.e3 PubMed31562902

2019

Rats provide a superior model of human stress erythropoiesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jingxin Zhang,Yijie Liu,Xu Han,Yang Mei,Jing Yang,Zheng J Zhang,Xinyan Lu,Peng Ji

Cell death & disease 10:691 PubMed31534118

2019

Exosomes derived from cardiac progenitor cells attenuate CVB3-induced apoptosis via abrogating the proliferation of CVB3 and modulating the mTOR signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Li,Zuocheng Yang,Wenyuan Nie,Jie Jiang,Shentang Li,Zhuoying Li,Lang Tian,Xing Ma

Disease models & mechanisms 11: PubMed30266803

2018

Generation of mouse-zebrafish hematopoietic tissue chimeric embryos for hematopoiesis and host-pathogen interaction studies.

Applications

Unspecified application

Species

Unspecified reactive species

Margarita Parada-Kusz,Cristina Penaranda,Elliott J Hagedorn,Anne Clatworthy,Anil V Nair,Jonathan E Henninger,Christoph Ernst,Brian Li,Raquel Riquelme,Humberto Jijon,Eduardo J Villablanca,Leonard I Zon,Deborah Hung,Miguel L Allende
View all publications
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