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AB48303

Biotin Anti-C Peptide antibody

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(1 Publication)

Rabbit Polyclonal Insulin antibody - conjugated to Biotin. Suitable for ELISA, RIA and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Insulin

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Reacts with

Human

Applications

ELISA, RIA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

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Product details

C Peptide is part of the molecule of Proinsulin, that consists of three parts: C Peptide and two long strands of amino acids (called the alpha and beta chains) that later become linked together to form the insulin molecule. From every molecule of proinsulin, one molecule of insulin plus one molecule of C Peptide are produced. C peptide is released into the blood stream in equal amounts to insulin. A test of C peptide levels will show how much insulin the body is making. Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.5 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C-peptide also known as connecting peptide is a short polypeptide chain consisting of 31 amino acids with a molecular weight of about 3 kDa. It results from enzymatic cleavage during the conversion of proinsulin to insulin and is co-secreted equimolarly with insulin by beta cells of the pancreas. C-peptide does not undergo significant liver metabolism making its levels a meaningful indicator of endogenous insulin production. This peptide is important due to its ability to serve as a biomarker for beta cell function giving insights into the body's insulin production capacity. Commercially available assays for its measurement include C-peptide ELISA and C-peptide ELISA kit important in both clinical and research settings.
Biological function summary

C-peptide plays a role beyond being a mere byproduct of insulin synthesis. Experiments have shown it binds to cell membranes indicating it functions independently and is more than part of the insulin processing complex. Studies suggest C-peptide may have physiological effects such as improving blood flow and possessing anti-inflammatory properties. Its ability to bind likely involves specific interactions that suggest possible receptors a subject of ongoing research. Detection methods like peptide ELISA and peptide test kit further facilitate studying its biological activity and potential therapeutic implications.

Pathways

C-peptide is closely linked to the metabolic insulin signaling pathway. This pathway is integral in glucose homeostasis and involves proteins such as insulin receptor and glucose transporter. Although not directly involved in the receptor-mediated actions of insulin C-peptide's presence alongside insulin shortly after release places it within this critical pathway. Another related pathway includes peptide hormone responses where C-peptide might influence vasodilation or cellular signaling revealing potential avenues for regulating metabolic conditions.

C-peptide is most notably associated with diabetes mellitus specifically type 1 and type 2. Its measurement helps to distinguish between the types of diabetes as it reflects the pancreas's ability to produce insulin. Lower levels often correlate with type 1 diabetes due to autoimmune destruction of beta cells whereas type 2 diabetes may still show normal C-peptide levels as the disease progresses. C-peptide also contributes to diabetic complications through associations with proteins involved in vascular function suggesting its possible role in conditions like diabetic nephropathy and retinopathy.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
See full target information INS

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Medicine 97:e11293 PubMed29953011

2018

Prevalence and factors associated with nonalcoholic fatty pancreas disease and its severity in China.

Applications

Unspecified application

Species

Unspecified reactive species

Siying Weng,Jianyang Zhou,Xiabo Chen,Yihong Sun,Zhujun Mao,Kefu Chai
View all publications

Product promise

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