JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB48342

Biotin Anti-C3 antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal C3 antibody - conjugated to Biotin. Suitable for IP, WB, RIA and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human C3.

View Alternative Names

CPAMD1, C3, Complement C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Reacts with

Human

Applications

RIA, WB, IP

applications

Immunogen

Native Full Length Protein corresponding to Human C3.

P01024

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "RIA" : {"fullname" : "Radioimmunoassay", "shortname":"RIA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "RIA-species-checked": "guaranteed", "RIA-species-dilution-info": "", "RIA-species-notes": "<p></p>" } } }
style
left-column

You may be interested in:

AB108823

Human Complement C3 ELISA Kit

5

2 Reviews

View product

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 49.73% PBS, 0.25% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Complement component 3 (C3) commonly known as C3 complement is a central protein in the complement system which plays a significant role in immune response. C3b a fragment of C3 is produced when C3 undergoes cleavage. C3 is a large protein with a mass of approximately 185 kDa. The liver primarily secretes C3 into the bloodstream. It circulates in the plasma and is found in high concentration making it one of the most abundant components of the complement system.
Biological function summary

Complement component C3 forms part of the innate immune system by promoting opsonization which enhances phagocytosis of pathogens. C3b binds to pathogens' surfaces facilitating their recognition by phagocytes. C3 as part of a complex with C3 convertase also has a role in amplifying the activation of the complement cascade. The proteolytic cleavage of C3 into C3b and C3a leads to the activation of other components forming the membrane attack complex and orchestrating inflammation.

Pathways

The complement component C3 functions within both the classical and alternative complement pathways. It acts as a convergence point where the complement activation pathways meet. C3 is activated into C3b and C3a which are key to amplifying the cascade. Furthermore C3 interacts with proteins such as factor B and factor D in the alternative pathway and C4 and C2 in the classical pathway facilitating the formation of C3 convertase necessary for pathway progression.

Complement C3 shows associations with immune-related and inflammatory diseases. Deficiencies or malfunctions of complement C3 can lead to increased susceptibility to infections due to impaired opsonization and clearance of pathogens. Additionally overactivation of the complement system involving C3 can contribute to autoimmune disorders such as systemic lupus erythematosus. Other proteins linked to these diseases include C4 in lupus and factor H in age-related macular degeneration which controls complement pathway activation.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.. Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils (By similarity). It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.. C3-beta-c. Acts as a chemoattractant for neutrophils in chronic inflammation.. Acylation stimulating protein. Adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-mediated internalization and recycling of C5AR2 (PubMed : 10432298, PubMed : 15833747, PubMed : 16333141, PubMed : 19615750, PubMed : 2909530, PubMed : 8376604, PubMed : 9059512).
See full target information C3
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

PloS one 16:e0259777 PubMed34767613

2021

Urinary C3 levels associated with sepsis and acute kidney injury-A pilot study.

Applications

Unspecified application

Species

Unspecified reactive species

Sahra Pajenda,Florence Zawedde,Sebastian Kapps,Ludwig Wagner,Alice Schmidt,Wolfgang Winnicki,David O'Connell,Daniela Gerges

PeerJ 7:e8218 PubMed31871840

2019

A novel approach to immunoapheresis of C3a/C3 and proteomic identification of associates.

Applications

Unspecified application

Species

Unspecified reactive species

Wolfgang Winnicki,Peter Pichler,Karl Mechtler,Richard Imre,Ines Steinmacher,Gürkan Sengölge,Daniela Knafl,Georg Beilhack,Ludwig Wagner
View all publications
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column

Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB198517

Alexa Fluor® 594 Anti-C3 antibody [EPR2988]

primary-antibodies

alexa-fluor-594-c3-antibody-epr2988-ab198517

0

(0 reviews)

Alternative Product
Primary Antibodies

AB196458

Alexa Fluor® 488 Anti-C3 antibody [EPR2988]

primary-antibodies

alexa-fluor-488-c3-antibody-epr2988-ab196458

0

(0 reviews)

Alternative Product
Primary Antibodies

AB200999

Anti-C3 antibody [EPR19394]

primary-antibodies

c3-antibody-epr19394-ab200999

4

(9 reviews)

Alternative Product
Primary Antibodies

AB181147

Anti-C3 antibody [EPR2988]

primary-antibodies

c3-antibody-epr2988-ab181147

5

(1 reviews)

Alternative Product
Primary Antibodies

AB240245

Anti-C3 antibody [EPR2988] - BSA and Azide free

primary-antibodies

c3-antibody-epr2988-bsa-and-azide-free-ab240245

0

(0 reviews)

Alternative Product
Primary Antibodies

AB196639

Alexa Fluor® 647 Anti-C3 antibody [EPR2988]

primary-antibodies

alexa-fluor-647-c3-antibody-epr2988-ab196639

0

(0 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com