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Mouse Monoclonal C5 antibody - conjugated to Biotin. Suitable for ELISA and reacts with Pig, Baboon, Human, Horse samples. Immunogen corresponding to Native Full Length Protein corresponding to Human C9.
Alternative names=Complement C5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4, CPAMD4, C5
- Isotype
IgG2a
- Host species
Mouse
- Conjugation
Biotin
- Storage buffer
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituents: 0.81% Sodium chloride, 0.16% Sodium phosphate- Form
Liquid
- Clonality
Monoclonal
- Immunogen
Native Full Length Protein corresponding to Human C9. Database link: P02748
Reactivity data
Select an application| ELISA | |
|---|---|
| Human | Expected |
| Baboon | Expected |
| Horse | Expected |
| Pig | Expected |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Pig, Baboon, Human, Horse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. Binding to the receptor C5AR1 induces a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes (PubMed:8182049). C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.
Recommended products
Mouse Monoclonal C5 antibody - conjugated to Biotin. Suitable for ELISA and reacts with Pig, Baboon, Human, Horse samples. Immunogen corresponding to Native Full Length Protein corresponding to Human C9.
Alternative names=Complement C5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4, CPAMD4, C5
Key facts
- Isotype
IgG2a
- Host species
Mouse
- Conjugation
Biotin
- Storage buffer
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituents: 0.81% Sodium chloride, 0.16% Sodium phosphate- Form
Liquid
- Clonality
Monoclonal
- Immunogen
Native Full Length Protein corresponding to Human C9. Database link: P02748
- Clone number
aE11
- Purification technique
Affinity purification
- Specificity
ab237699 binds both membrane-bound MAC (active) and fluid-phase SC5b-9 complexes (inactive).
The aE11 monoclonal was first reported to bind to a neoepitope exposed on polymerized C9 when incorporated into the human terminal complement complex (PubMed ID: 4035298). It was subsequently shown that this antibody also cross reacts with the C5b-8 complex and purified C8 alpha-gamma. It does not react with native C9 or C8 proteins (PubMed ID: 2424021).
- Epitope
ab237699 binds to a neoepitope exposed on C9.
- Light chain type
kappa
- Concentration
- Purification notes
Biotinylated
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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