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AB237699

Biotin Anti-C5b-9 + C5b-8 antibody [aE11]

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(2 Publications)

Anti-C5b-9 + C5b-8 antibody [aE11] - Biotin conjugated (ab237699) is a mouse monoclonal antibody detecting C5b-8 in ELISA. Suitable for Baboon, Horse, Human, Pig.

View Alternative Names

CPAMD4, C5, Complement C5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4, Complement component C7, C7, Complement component C8 alpha chain, Complement component 8 subunit alpha, C8A, Complement component C9, C9, Complement component C6, C6

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

aE11

Isotype

IgG2a

Light chain type

kappa

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Reacts with

Horse, Human, Pig, Baboon

Applications

ELISA

applications

Immunogen

Native Full Length Protein corresponding to Human C9.

P02748

Epitope

ab237699 binds to a neoepitope exposed on C9.

Specificity

ab237699 binds both membrane-bound MAC (active) and fluid-phase SC5b-9 complexes (inactive).

The aE11 monoclonal was first reported to bind to a neoepitope exposed on polymerized C9 when incorporated into the human terminal complement complex (PubMed ID: 4035298). It was subsequently shown that this antibody also cross reacts with the C5b-8 complex and purified C8 alpha-gamma. It does not react with native C9 or C8 proteins (PubMed ID: 2424021).

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Alternative Products

Primary Antibodies

AB55811

Anti-C5b-9 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5b-9 antibody (AB55811)

  • 3
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Complementary Products

Assay Kits

AB125963

Human Complement C5 ELISA Kit

Sandwich ELISA - Human Complement C5 ELISA Kit (AB125963)

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Primary Antibodies

AB196458

Alexa Fluor® 488 Anti-C3 antibody [EPR2988]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-C3 antibody [EPR2988] (AB196458)

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Primary Antibodies

AB173302

Anti-C9 antibody [EPR11232-82]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C9 antibody [EPR11232-82] (AB173302)

  • 0
  • 0
Primary Antibodies

AB55811

Anti-C5b-9 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5b-9 antibody (AB55811)

  • 3
  • 4
Primary Antibodies

AB190834

Anti-MBL1+MBL2 antibody [EPR18381-156]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBL1+MBL2 antibody [EPR18381-156] (AB190834)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>" }, "Baboon": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>" }, "Horse": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>" }, "Pig": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Biotin Anti-C5b-9 + C5b-8 antibody [aE11] (ab237699) is a mouse monoclonal antibody and is validated for use in ELISA in Baboon, Horse, Human, Pig samples.

Related products
Antibody clone aE11 is also available pre-conjugated to a variety of labels for your convenience – Anti-C5b-8 Biotin C5b-9 + [aE11] (ab237699).

Other related products
We have a range of other formats of antibody clone [aE11] also available for your convenience: ab66768, Biotin - ab237699, ab309530
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Biotinylated
Storage buffer
pH: 7.4 Preservative: 0.0975% Sodium azide Constituents: 0.81% Sodium chloride, 0.16% Sodium phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The C5b-9 complex also known as the membrane attack complex (MAC) plays an important role in the immune system specifically in the complement cascade. This complex forms as a result of the sequential interaction of complement proteins C5b C6 C7 C8 and multiple C9 molecules creating a pore in the membrane of target cells leading to cell lysis. The C5b-8 serves as an intermediary in the formation of the C5b-9 complex and contributes to the initial assembly of the membrane attack complex. Expression of these components predominantly occurs on the surface of lymphocytes and activated macrophages though the exact mass of the assembled complex is less often quoted in standard references.
Biological function summary

C5b-9 disrupts cellular homeostasis by forming transmembrane channels that permit unregulated ion movement. This function is pivotal in innate immunity where it eliminates virally infected and neoplastic cells. The C5b-9 complex forms through progressive recruitment and insertion of complement proteins into pathogenic membranes with each molecule one step closer to full assembly and execution of its lytic activity. In many immune system-related literature it represents terminal complement complexation signifying the endpoint of complement-mediated cytotoxicity.

Pathways

C5b-9 is an integral component of the complement system's terminal pathway. This pathway escalates from the activation of C3 to the cleavage of C5 with C5b serving as an anchor for further protein assembly. The complement cascade also intersects with coagulation pathways bridging roles between immune defense and clotting functions. In this pathway association with proteins such as C3b and C6 is essential for initiating and propagating complex formation and ultimately cellular destruction.

The C5b-9 complex has notable involvement in autoimmune diseases and renal disorders. Paroxysmal nocturnal hemoglobinuria a condition characterized by red blood cell lysis directly involves excessive C5b-9 deposition due to defective complement regulation on host cells. Similarly atypical hemolytic uremic syndrome which results in kidney damage often links to mutations affecting regulators of C5b-9 activity showing the complex's contribution to pathogenesis. Employing therapeutic strategies researchers often target components like C5 and C9 to mitigate the harmful overactivation or misdirection of this complement activity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Precursor of the C5a anaphylatoxin and complement C5b components of the complement pathways, which consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 12878586, PubMed : 18204047, PubMed : 30643019, PubMed : 6554279). Activated downstream of classical, alternative, lectin and GZMK complement pathways (PubMed : 12878586, PubMed : 18204047, PubMed : 30643019, PubMed : 39914456, PubMed : 39814882, PubMed : 6554279).. Complement C5b. Component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis (PubMed : 26841837, PubMed : 27052168, PubMed : 30552328, PubMed : 30643019). Complement C5b is generated following cleavage by C5 convertase and initiates formation of the MAC complex : C5b binds sequentially C6, C7, C8 and multiple copies of the pore-forming subunit C9 (PubMed : 30552328, PubMed : 30643019). During MAC complex assembly, the C5b6 subcomplex, composed of complement C5b and C6, associates with the outer leaflet of target cell membrane, reducing the energy for membrane bending (PubMed : 30552328, PubMed : 32569291).. C5a anaphylatoxin. Mediator of local inflammatory process released following cleavage by C5 convertase (PubMed : 8182049, PubMed : 9553099). Acts by binding to its receptor (C5AR1 or C5AR2), activating G protein-coupled receptor signaling and inducing a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes (PubMed : 36806352, PubMed : 37852260, PubMed : 37169960, PubMed : 8182049, PubMed : 9553099). C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation (PubMed : 342601, PubMed : 37852260, PubMed : 37169960, PubMed : 5765461, PubMed : 8182049, PubMed : 9553099).
See full target information C5

Additional targets

C7,C8A,C6,C9
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 301:108250 PubMed39894217

2025

Modulating the complement system through epitope-specific inhibition by complement C3 inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Zhidong Chen,Mingshuang Wang,Wenqian Duan,Yi Xia,Huiqin Liu,Feng Qian

Translational cancer research 13:81-101 PubMed38410208

2024

Validation of the interaction between PRDX4 and TXNDC5 in gastric cancer and the significance of the gene in gastric cancer based on a data mining analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Zhang,Kai Wu,Yanhong Hou,Xianghui Li
View all publications
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