Rabbit Recombinant Monoclonal Caspase-3 antibody - conjugated to Biotin. Suitable for IHC-P, WB and reacts with Human, Synthetic peptide - Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Caspase-3 antibody [E87] (AB195905), expandable thumbnail](https://content.abcam.com/products/images/biotin-caspase-3-antibody-e87-ab195905--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img69856.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Caspase-3 antibody [E87] (AB195905)")
![Western blot - Biotin Anti-Caspase-3 antibody [E87] (AB195905), expandable thumbnail](https://content.abcam.com/products/images/biotin-caspase-3-antibody-e87-ab195905--western-blot-img88242.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Biotin Anti-Caspase-3 antibody [E87] (AB195905)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
Biotin
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Expected |
| Synthetic peptide - Human | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info 1/10000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
9 products for Alternative Product
1 product for Alternative Version
Target data
Function
Involved in the activation cascade of caspases responsible for apoptosis execution (PubMed:7596430). At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond (PubMed:7774019). Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9 (PubMed:7596430). Involved in the cleavage of huntingtin (PubMed:8696339). Triggers cell adhesion in sympathetic neurons through RET cleavage (PubMed:21357690). Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress (PubMed:23152800).
Alternative names
Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, Protein Yama, SREBP cleavage activity 1, CPP-32, SCA-1, CASP3, CPP32
Recommended products
Rabbit Recombinant Monoclonal Caspase-3 antibody - conjugated to Biotin. Suitable for IHC-P, WB and reacts with Human, Synthetic peptide - Human samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
Biotin
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
E87
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle, Store in the dark
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Caspase-3 antibody [E87] (ab195905), expandable thumbnail](https://content.abcam.com/products/images/biotin-caspase-3-antibody-e87-ab195905--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img69856.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Caspase-3 antibody [E87] (ab195905)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Caspase-3 antibody [E87] (ab195905)
IHC image of Caspase-3 staining in a section of formalin-fixed paraffin-embedded Human normal tonsil*, performed on a Leica BondTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins, before blocking of endogenous biotin using Avidin/Biotin Blocking Kit ab64212. The section was then incubated with ab195905, 1/50 dilution, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
![Western blot - Biotin Anti-Caspase-3 antibody [E87] (ab195905), expandable thumbnail](https://content.abcam.com/products/images/biotin-caspase-3-antibody-e87-ab195905--western-blot-img88242.jpg "Western blot - Biotin Anti-Caspase-3 antibody [E87] (ab195905)")
Western blot - Biotin Anti-Caspase-3 antibody [E87] (ab195905)
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab195905 overnight at 4°C. Antibody binding to the target was detected using a HRP conjugated streptavidin amplification step, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - Biotin Anti-Caspase-3 antibody [E87] (ab195905) at 1/10000 dilution
All lanes: Western blot - Recombinant human Cleaved Caspase-3 protein (Active) (Recombinant human Cleaved Caspase-3 protein (Active) ab52101) at 0.1 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 15 kDa
Exposure time: 10s
Downloads
Product protocols
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