Rabbit Polyclonal CCL21 antibody - conjugated to Biotin. Suitable for WB, sELISA and reacts with Recombinant full length protein - Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Mouse Ccl21c aa 1 to C-terminus.
View Alternative Names
Scya21c, Ccl21c, C-C motif chemokine 21c, 6Ckine, Beta-chemokine exodus-2, Small-inducible cytokine A21c, Thymus-derived chemotactic agent 4, TCA4
- sELISA
Supplier Data
Sandwich ELISA - Biotin Anti-CCL21 antibody (AB271203)
Mouse CCL21 was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271203.
This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mouse Exodus-2 protein.
- WB
Supplier Data
Western blot - Biotin Anti-CCL21 antibody (AB271203)
To detect CCL21 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant CCL21 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Lane 1 : Marker
Lanes 2-12 : 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant mouse Exodus 2, respectively.
Non-reducing conditions.
All lanes:
Western blot - Biotin Anti-CCL21 antibody (ab271203)
Predicted band size: 15 kDa
false
Complementary Products
Reactivity data
Properties and storage information
Form
Reconstitution
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CCL21 is essential for guiding immune cells to areas where they are needed for initiating immune responses. It forms a non-covalent complex with its receptor CCR7 a primary mechanism through which it exerts its biological effects. CCL21 coordinates the positioning of immune cells within the lymphoid organs ensuring a functional and effective immune response to pathogens or injuries. Its expression pattern aids in the organization of lymphoid tissue architecture and contributes to efficient trafficking of T-cells and dendritic cells.
Pathways
CCL21 plays an important role in the chemokine signaling pathway and the leukocyte transendothelial migration pathway. Within these pathways CCL21 works closely with its receptor CCR7 to guide immune cell navigation and localization. This collaboration is essential for directing cells to appropriate sites in the immune system aligning CCL21 with similar proteins like CCL19 another chemokine that also interacts with CCR7.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com