AB83139

Biotin Anti-CCL3 / MIP-1 alpha antibody

Name: Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 Biotin antibody (ab83139) | Abcam
Brand: Abcam Limited
SKU: AB83139
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal MIP-1 alpha/CCL3 antibody - conjugated to Biotin. Carrier free. Suitable for WB, sELISA and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Ccl3.

View Alternative Names

Mip1a, Scya3, Ccl3, C-C motif chemokine 3, Heparin-binding chemotaxis protein, L2G25B, Macrophage inflammatory protein 1-alpha, SIS-alpha, Small-inducible cytokine A3, TY-5, MIP-1-alpha

2 Images

Sandwich ELISA - Biotin Anti-CCL3 / MIP-1 alpha antibody (AB83139)

sELISA

Supplier Data

Sandwich ELISA - Biotin Anti-CCL3 / MIP-1 alpha antibody (AB83139)

To detect murine Macrophage Inflammatory Protein 1 alpha / CCL3 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab83139 is required. This biotinylated polyclonal antibody, in conjunction with an anti-Macrophage Inflammatory Protein 1 alpha / CCL3 (ab9927) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant murine Macrophage Inflammatory Protein 1 alpha / CCL3.

Supplier Data

Western blot - Biotin Anti-CCL3 / MIP-1 alpha antibody (AB83139)

To detect murine Macrophage Inflammatory Protein 1 alpha / CCL3 by Western Blot analysis ab83139 can be used at a concentration of 0.1 - 0.2 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant murine Macrophage Inflammatory Protein 1 alpha / CCL3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Image shown is non-reducing conditions; Murine recombinant Macrophage Inflammatory Protein 1 alpha / CCL3 protein at 250 - 0.24 ng/ml.

Lane 1 is MW marker, lanes 2-12 murine recombinant Macrophage Inflammatory Protein 1 alpha / CCL3 protein.

All lanes:

Western blot - Biotin Anti-CCL3 / MIP-1 alpha antibody (ab83139)

Predicted band size: 10 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Excitation/Emission

Carrier free

Yes

Reacts with

Mouse

Applications

WB, sELISA

applications

Immunogen

Recombinant Full Length Protein corresponding to Mouse Ccl3.

P10855

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.2 µg/mL", "WB-species-notes": "<p>The detection limit for recombinant murine Inflammatory Protein 1 alpha / CCL3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.</p>", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "0.25-1 µg/mL", "sELISA-species-notes": "<p>Allows the detection of at least 0.2 - 0.4 ng/well of recombinant murine Macrophage Inflammatory Protein 1 alpha / CCL3.</p>" } } }

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Properties and storage information

Form

Lyophilized

Reconstitution

Reconstitute in PBS, 0.1% BSA

Purification technique

Affinity purification Protein A

Purification notes

ab83139 is sterile filtered.

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MIP-1 alpha also known as CCL3 is a chemokine that plays a critical role in the immune system. With a molecular weight of around 8 kDa it is primarily expressed in activated T-cells macrophages and fibroblasts. MIP-1 alpha acts as a signaling protein that binds to receptors on target cells directing the movement of immune cells. Researchers commonly refer to it using various alternative names such as MIP-1a and CCL3-alpha rat and it is frequently measured using assays like MIP-1 alpha ELISA for experimental analyses.

Biological function summary

Proteins including MIP-1 alpha participate in the recruitment and activation of diverse immune cells aiding the inflammatory response. Through interaction with receptors like CCR1 and CCR5 MIP-1 alpha mobilizes cells like natural killer cells and monocytes. This chemokine does not generally form part of a larger protein complex but interacts directly with membrane receptors to exert its function in immune cell chemotaxis.

Pathways

MIP-1 alpha operates in the context of both the inflammatory response and the chemokine signaling pathways. Its involvement in these pathways helps mediate the trafficking of leukocytes. Notably MIP-1 alpha interacts with proteins such as CCR1 and CCR5 to transmit signals that instigate immune responses. These proteins are integral to its role in modulating immunity providing targets for research into controlling inflammatory processes.

MIP-1 alpha closely relates to conditions such as rheumatoid arthritis and HIV infection. In rheumatoid arthritis its overexpression can lead to excessive inflammation and joint destruction. Meanwhile in HIV infection MIP-1 alpha interacts with CCR5 a co-receptor necessary for the virus entry into cells. This dual relationship elucidates its potential as a therapeutic target in managing both inflammatory diseases and viral entry mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).

See full target information Ccl3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental dermatology 32:403-412 PubMed36457234

2022

CCL6 promotes M2 polarization and inhibits macrophage autophagy by activating PI3-kinase/Akt signalling pathway during skin wound healing.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Feng,Yu Ji,Ce Zhang,Tingting Jin,Jingyu Li,Jincai Guo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-CCL3 / MIP-1 alpha antibody

Sandwich ELISA - Biotin Anti-CCL3 / MIP-1 alpha antibody (AB83139)

Western blot - Biotin Anti-CCL3 / MIP-1 alpha antibody (AB83139)

Key facts

Host species

Clonality

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Reconstitution

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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