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AB245844

Biotin Anti-CD137 antibody

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(1 Publication)

Goat Polyclonal CD137 antibody - conjugated to Biotin. Suitable for WB, sELISA and reacts with Recombinant fragment - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TNFRSF9 aa 1-200.

View Alternative Names

CD137, ILA, TNFRSF9, Tumor necrosis factor receptor superfamily member 9, 4-1BB ligand receptor, CDw137, T-cell antigen 4-1BB homolog, T-cell antigen ILA

2 Images
Sandwich ELISA - Biotin Anti-CD137 antibody (AB245844)
  • sELISA

Supplier Data

Sandwich ELISA - Biotin Anti-CD137 antibody (AB245844)

To detect human CD137 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab245844 is required.

This biotinylated polyclonal antibody, in conjunction with a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant human CD137.

Western blot - Biotin Anti-CD137 antibody (AB245844)
  • WB

Supplier Data

Western blot - Biotin Anti-CD137 antibody (AB245844)

To detect human CD137 by Western Blot analysis ab245844 can be used at a concentration of 0.1 - 0.2 μg/ml.

Used in conjunction with compatible secondary reagents the detection limit for recombinant human CD137 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Lanes 1-11 : 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant human CD137, respectively.

Non-reducing conditions.

All lanes:

Western blot - Biotin Anti-CD137 antibody (ab245844)

Predicted band size: 28 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Applications

WB, sELISA

applications

Immunogen

Recombinant Fragment Protein within Human TNFRSF9 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q07011

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Complementary Products

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Proteins & Peptides

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Primary Antibodies

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Primary Antibodies

AB281764

Alexa Fluor® 555 Anti-CD16 antibody [EPR16784]

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "sELISA-species-checked": "predicted", "sELISA-species-dilution-info": "", "sELISA-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.2 µg/mL", "WB-species-notes": "<p></p>", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "0.25-1 µg/mL", "sELISA-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Lyophilized
Reconstitution
Reconstitute in PBS, 0.1% BSA
Purification technique
Affinity purification
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD137 also known as 4-1BB or TNFRSF9 is a protein that functions as a costimulatory receptor in the immune system. It belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD137 has a mass of approximately 30 kDa. Expression of CD137 occurs mainly on activated T lymphocytes including both CD8+ and CD4+ T cells as well as natural killer (NK) cells dendritic cells and monocytes. The CD137 ligand (CD137L) is the counterpart that engages this receptor transmitting signals important for immune response regulation.
Biological function summary

CD137 plays an important role in T cell activation and survival. It is a member of a receptor-ligand system that enhances immune cell functions. CD137 is part of a receptor complex that when bound by its ligand CD137L promotes cell proliferation and cytokine production. It also supports the development of memory T cells contributing to the body's ability to mount long-term immune responses.

Pathways

CD137 takes part in the NF-kB signaling and MAPK pathways. These pathways are vital for regulating immune responses and inflammation. CD137 signaling through these pathways involves interaction with proteins such as TRAF1 and TRAF2 which help mediate downstream effects critical for cell survival and proliferation. These interactions enhance the body's defense against pathogens and tumors.

CD137 is associated with cancer immunotherapy and autoimmune diseases. In cancer CD137 enhances the anti-tumor activity of immune cells making it a target for immunotherapy strategies. For autoimmune diseases abnormal CD137 expression can lead to excessive immune responses contributing to disease pathogenesis. The protein is also connected to TNF-alpha through its involvement in inflammation suggesting potential intervention points for modulating immune response in related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for TNFSF9/4-1BBL. Conveys a signal that enhances CD8(+) T-cell survival, cytotoxicity, and mitochondrial activity, thereby promoting immunity against viruses and tumors (Probable).
See full target information TNFRSF9
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 22:563 PubMed39272146

2024

Circ_0001947 encapsulated by small extracellular vesicles promotes gastric cancer progression and anti-PD-1 resistance by modulating CD8 T cell exhaustion.

Applications

Unspecified application

Species

Unspecified reactive species

Bingyu Wang,Wenbo Liu,Mingming Zhang,Yong Li,Hongyue Tang,Yingying Wang,Chao Song,Buyun Song,Bibo Tan
View all publications
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Product promise

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