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Mouse Monoclonal CD14 antibody - conjugated to Biotin. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Native Full Length Protein corresponding to Human CD14.

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Images

Flow Cytometry - Biotin Anti-CD14 antibody [MEM-18] (AB21889), expandable thumbnail
  • Flow Cytometry - Biotin Anti-CD14 antibody [MEM-18] (AB21889), expandable thumbnail

Key facts

Isotype

IgG1

Host species

Mouse

Conjugation

Biotin

Storage buffer

pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Native Full Length Protein corresponding to Human CD14. Database link P08571

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt
Human
Tested
Primates
Predicted

Tested
Tested

Species

Human

Dilution info

2-4 µg/mL

Notes

-

Predicted
Predicted

Species

Primates

Dilution info

-

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Version

6 products for Alternative Product

Target data

Function

Coreceptor for bacterial lipopolysaccharide (PubMed:1698311, PubMed:23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:20133493, PubMed:22265692, PubMed:23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135). Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211). Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (PubMed:23880187).

Alternative names

Recommended products

Mouse Monoclonal CD14 antibody - conjugated to Biotin. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Native Full Length Protein corresponding to Human CD14.

Key facts

Isotype

IgG1

Conjugation

Biotin

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Native Full Length Protein corresponding to Human CD14. Database link P08571
Clone number

MEM-18

Purification technique

Affinity purification Protein G

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Do Not Freeze

Notes

Purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD14 also known as cluster of differentiation 14 is a glycoprotein with a molecular mass of approximately 55 kDa. This protein is expressed mainly on the surface of macrophages monocytes and to a lesser extent on neutrophils. CD14 exists in two forms: membrane-bound (mCD14) and soluble (sCD14). It functions as a co-receptor along with TLR4 (Toll-like receptor 4) and MD-2 for the detection of bacterial lipopolysaccharide (LPS) which is an important component of the outer membrane of Gram-negative bacteria.

Biological function summary

This glycoprotein plays a significant role in the innate immune system by recognizing pathogen-associated molecular patterns (PAMPs). It is part of a receptor complex where CD14 acts alongside LPS binding protein (LPS-R) TLR4 and MD-2 to facilitate pro-inflammatory signaling in response to microbial invasion. By binding to LPS CD14 initiates and amplifies the immune response leading to the production of cytokines and the recruitment of other immune cells to the site of infection.

Pathways

CD14 interacts with the toll-like receptor signaling pathway notably involving TLR4. Upon LPS detection CD14 assists in the activation of TLR4 triggering downstream signaling cascades such as NF-κB and MAPK pathways. These pathways result in the transcription of inflammatory cytokines and are essential for the host defense mechanism. The protein complex involving CD14 further interacts with adaptor proteins like MyD88 and TRIF facilitating signal propagation and the immune system's ability to respond to pathogens.

Associated diseases and disorders

Researchers have linked CD14 to sepsis and atherosclerosis. High levels of soluble CD14 (sCD14) are often observed in patients with sepsis indicating its participation in excessive systemic inflammation. In atherosclerosis CD14's role in recognizing LPS contributes to chronic inflammation and the development of plaques in arteries. Through these diseases CD14 connects with TLR4 emphasizing its impact on inflammatory responses and its potential as a therapeutic target.

Product promise

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2 product images

  • Flow Cytometry - Biotin Anti-CD14 antibody [MEM-18] (ab21889), expandable thumbnail

    Flow Cytometry - Biotin Anti-CD14 antibody [MEM-18] (ab21889)

    Flow cytometry surface staining pattern of human peripheral whole blood stained using ab21889 at 6 μg/ml.

  • Flow Cytometry - Biotin Anti-CD14 antibody [MEM-18] (ab21889), expandable thumbnail

    Flow Cytometry - Biotin Anti-CD14 antibody [MEM-18] (ab21889)

    Separation of human monocytes (red) from lymphocytes (black) in flow cytometry analysis (surface staining) of human peripheral whole blood using ab21889 at 6 μg/ml, Streptavidin.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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