Mouse Monoclonal CD14 antibody - conjugated to Biotin. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Native Full Length Protein corresponding to Human CD14.
IgG1
Mouse
Biotin
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS
Liquid
Monoclonal
Flow Cyt | |
---|---|
Human | Tested |
Primates | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 2-4 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Primates | Dilution info - | Notes - |
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Coreceptor for bacterial lipopolysaccharide (PubMed:1698311, PubMed:23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:20133493, PubMed:22265692, PubMed:23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135). Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211). Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (PubMed:23880187).
Monocyte differentiation antigen CD14, My23 antigen, Myeloid cell-specific leucine-rich glycoprotein, CD14
Mouse Monoclonal CD14 antibody - conjugated to Biotin. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Native Full Length Protein corresponding to Human CD14.
IgG1
Mouse
Biotin
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS
Liquid
Monoclonal
MEM-18
Affinity purification Protein G
Blue Ice
1-2 weeks
+4°C
-20°C
Do Not Freeze
Purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
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This supplementary information is collated from multiple sources and compiled automatically.
CD14 also known as cluster of differentiation 14 is a glycoprotein with a molecular mass of approximately 55 kDa. This protein is expressed mainly on the surface of macrophages monocytes and to a lesser extent on neutrophils. CD14 exists in two forms: membrane-bound (mCD14) and soluble (sCD14). It functions as a co-receptor along with TLR4 (Toll-like receptor 4) and MD-2 for the detection of bacterial lipopolysaccharide (LPS) which is an important component of the outer membrane of Gram-negative bacteria.
This glycoprotein plays a significant role in the innate immune system by recognizing pathogen-associated molecular patterns (PAMPs). It is part of a receptor complex where CD14 acts alongside LPS binding protein (LPS-R) TLR4 and MD-2 to facilitate pro-inflammatory signaling in response to microbial invasion. By binding to LPS CD14 initiates and amplifies the immune response leading to the production of cytokines and the recruitment of other immune cells to the site of infection.
CD14 interacts with the toll-like receptor signaling pathway notably involving TLR4. Upon LPS detection CD14 assists in the activation of TLR4 triggering downstream signaling cascades such as NF-κB and MAPK pathways. These pathways result in the transcription of inflammatory cytokines and are essential for the host defense mechanism. The protein complex involving CD14 further interacts with adaptor proteins like MyD88 and TRIF facilitating signal propagation and the immune system's ability to respond to pathogens.
Researchers have linked CD14 to sepsis and atherosclerosis. High levels of soluble CD14 (sCD14) are often observed in patients with sepsis indicating its participation in excessive systemic inflammation. In atherosclerosis CD14's role in recognizing LPS contributes to chronic inflammation and the development of plaques in arteries. Through these diseases CD14 connects with TLR4 emphasizing its impact on inflammatory responses and its potential as a therapeutic target.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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Flow cytometry surface staining pattern of human peripheral whole blood stained using ab21889 at 6 μg/ml.
Separation of human monocytes (red) from lymphocytes (black) in flow cytometry analysis (surface staining) of human peripheral whole blood using ab21889 at 6 μg/ml, Streptavidin.
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