AB28103

Biotin Anti-CD15 antibody [MEM-158]

Name: Anti-CD15 Biotin antibody [MEM-158] (ab28103) Mouse monoclonal IgM | Abcam
Brand: Abcam Limited
SKU: AB28103
Price: 520 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal CD15 antibody - conjugated to Biotin. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing CD15 protein.

2 Images

Flow Cytometry - Biotin Anti-CD15 antibody [MEM-158] (AB28103)

Flow Cyt

Unknown

Flow Cytometry - Biotin Anti-CD15 antibody [MEM-158] (AB28103)

Separation of human neutrophil granulocytes (red-filled) from CD15 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood using ab28103 at 2 μg/ml.

Flow Cyt

Unknown

Flow Cytometry - Biotin Anti-CD15 antibody [MEM-158] (AB28103)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab28103 at 2 μg/ml.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-158

Isotype

IgM

Light chain type

kappa

Conjugation

Biotin

Excitation/Emission

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is specific to CD15 antigen (Lewis^x blood group antigen). We have data to indicate that this antibody may not cross react with Pig. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-4 µg/mL", "FlowCyt-species-notes": "<p><a href='/en-us/products/unavailable/mouse-igm-lambda-monoclonal-mopc-104e-biotin-isotype-control-ab18396'>ab18396</a> - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.</p>" } } }

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Product details

The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Purification notes

Purity >95% by SDS-PAGE.

Storage buffer

pH: 8 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD15 also known as Lewis x or 3-fucosyl-N-acetyllactosamine is a carbohydrate antigen with a molecular mass typically not individually defined due to its nature as a glycan. CD15 is expressed on the surface of many human cells including neutrophils and various epithelial cells. It is commonly associated with granulocytes and is used as a cell surface marker. The presence of CD15 can be identified using CD15 antibodies through techniques such as CD15 flow cytometry and CD15 APC labeling.

Biological function summary

CD15 is important in cell-cell interaction mechanisms. It plays a role in mediating adhesion processes particularly in immune responses where it aids neutrophils in adhering to vascular endothelium and transmigrating to sites of infection or inflammation. As a glycoconjugate CD15 forms part of a larger glycoprotein complex on the cell surface interacting with other proteins to facilitate cellular responses and movement.

Pathways

CD15's function as an adhesion molecule ties into key cellular processes such as the selectin pathway. In this pathway it interacts with proteins like E-selectin and L-selectin contributing to leukocyte rolling and extravasation during the immune response. Additionally CD15 is implicated in the signaling pathways that mediate inflammatory responses where its role in neutrophil dynamics is important.

CD15 has notable implications in Hodgkin's lymphoma and certain myeloid leukemias. It is frequently used as a diagnostic marker in these conditions where its overexpression or abnormal expression patterns may signify disease presence or progression. CD15's interaction with other proteins such as CD30 in Hodgkin's lymphoma highlights its utility in understanding the pathological mechanisms and potential therapeutic targets of these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information CD15

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Acta pharmaceutica Sinica. B 9:794-808 PubMed31384539

2019

Development of the triazole-fused pyrimidine derivatives as highly potent and reversible inhibitors of histone lysine specific demethylase 1 (LSD1/KDM1A).

Applications

Unspecified application

Species

Unspecified reactive species

Zhonghua Li,Lina Ding,Zhongrui Li,Zhizheng Wang,Fengzhi Suo,Dandan Shen,Taoqian Zhao,Xudong Sun,Junwei Wang,Ying Liu,Liying Ma,Bing Zhao,Pengfei Geng,Bin Yu,Yichao Zheng,Hongmin Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-CD15 antibody [MEM-158]

Flow Cytometry - Biotin Anti-CD15 antibody [MEM-158] (AB28103)

Flow Cytometry - Biotin Anti-CD15 antibody [MEM-158] (AB28103)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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