AB28093

Biotin Anti-CD18 antibody [MEM-48]

Name: Biotin Anti-CD18 antibody [MEM-48] (ab28093) | Abcam
Brand: Abcam Limited
SKU: AB28093
Price: 520 USD
Availability: InStock

Be the first to review this product! Submit a review

(1 Publication)

Mouse Monoclonal CD18 antibody - conjugated to Biotin. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing ITGB2 protein.

View Alternative Names

CD18, MFI7, ITGB2, Integrin beta-2, Complement receptor C3 subunit beta

1 Images

Flow Cytometry - Biotin Anti-CD18 antibody [MEM-48] (AB28093)

Flow Cyt

Supplier Data

Flow Cytometry - Biotin Anti-CD18 antibody [MEM-48] (AB28093)

Flow cytometry analysis (surface staining) of human peripheral blood with ab28093.

Carrier free

Anti-CD18 antibody [MEM-48] - Low endotoxin, Azide free
Unconjugated

Anti-CD18 antibody [MEM-48]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-48

Isotype

IgG1

Conjugation

Biotin

Excitation/Emission

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing ITGB2 protein. The exact immunogen used to generate this antibody is proprietary information.

P05107

Epitope

Ab28093 reacts with an epitope located in the region encoded by aa 534-546 in cysteine rich repeat 3 of the CD18 antigen.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-3 µg/mL", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/biotin-mouse-igg1-kappa-monoclonal-mopc-21-isotype-control-ab18434'>ab18434</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

style

left-column

Product details

The antibody is free of unconjugated biotin.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Purification notes

Purity >95% by SDS-PAGE.

Storage buffer

pH: 8 Preservative: 0.097% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD18 also known as ITGB2 is a protein with a molecular weight of approximately 95 kDa. It is part of the beta-2 integrin family and partners with different alpha subunits to form heterodimers. CD18 is primarily expressed on leukocytes including neutrophils monocytes macrophages and lymphocytes. As a surface marker CD18 plays a significant role in immune cell adhesion and migration enabling cells to respond to inflammation signals effectively.

Biological function summary

The CD18 protein functions in conjunction with associated alpha subunit partners forming a part of the wider integrin family complex. ItgB2 as it is sometimes referred to is essential for cell adhesion processes. These heterodimers facilitate binding between leukocytes and various ligands on endothelial cells which subsequently controls leukocyte transmigration through vascular walls. CD18's activities are critical in immune system responses making it a prominent focus in immunological research.

Pathways

The role of CD18 extends into inflammatory and immune response signaling pathways. It contributes critically to the leukocyte adhesion cascade. This cascade engagement involves interaction with other proteins such as ICAM-1 on endothelial surfaces. CD18 through its association in integrin-mediated signaling influences processes like phagocytosis and lymphocyte activation giving it a central place in cellular immunity.

Deficiencies or mutations affecting CD18 function correlate with disorders such as Leukocyte Adhesion Deficiency type I (LAD-I). This condition results from impaired leukocyte function due to defective integrin expression. The role of CD18 in arthritis is also significant primarily by mediating leukocyte infiltration into inflamed tissues. Its interaction with proteins like ICAM-1 becomes exceptionally relevant in these disease dynamics indicating potential therapeutic targets for modulating immune responses.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Integrin ITGAL : ITGB2 is a receptor for ICAM1, ICAM2 and ICAM3 (PubMed : 1676048, PubMed : 23775590, PubMed : 38195629). Integrin ITGAL : ITGB2 is also a receptor for the secreted form of ubiquitin-like protein ISG15; the interaction is mediated by ITGAL (PubMed : 29100055). Integrins ITGAM : ITGB2 and ITGAX : ITGB2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin ITGAX : ITGB2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin ITGAM : ITGB2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin ITGAM : ITGB2 is also a receptor for factor X. Integrin ITGAD : ITGB2 is a receptor for ICAM3 and VCAM1 (PubMed : 10438935, PubMed : 8777714, PubMed : 9841932). Contributes to natural killer cell cytotoxicity (PubMed : 15356110). Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils (PubMed : 11812992, PubMed : 28807980). Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation (PubMed : 18587400). Integrin ITGAL : ITGB2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed : 23775590). In association with alpha subunit ITGAM/CD11b, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed : 21193407). Integrins ITGAX : ITGB2 functions as a receptor of the erythrocyte-specific adhesion molecule ICAM4 and mediates erythrophagocytosis (PubMed : 16985175). Integrins ITGAX : ITGB2 functions as a receptor of the neuron-specific adhesion molecule ICAM5 ensuring neuron cell-leukocyte adhesion (PubMed : 10741396). Integrin ITGAL : ITGB2 functions as a receptor of ICAM1 by acting as a platform at the immunological synapse to translate TCR engagement and density of the ITGAL ligand ICAM1 into graded adhesion (PubMed : 38195629). Integrin ITGAM : ITGB2/MAC-1 complex functions as a signaling receptor for the ligand receptor ICAM1, ensuring adhesion between stimulated neutrophils and stimulated endothelial cells (PubMed : 1980124). Integrin ITGAL/ITGB2 that functions as a signaling receptor of ICAM2, ensuring leukocyte cell-cell adhesion on resting cells (PubMed : 1676048).

See full target information ITGB2

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Folia biologica 36:41-50 PubMed1971601

1990

Monoclonal antibodies against human leucocyte antigens. IV. Antibodies against subunits of the LFA-1 (CD11a/CD18) leucocyte-adhesion glycoprotein.

Applications

Unspecified application

Species

Unspecified reactive species

V Bazil,I Stefanová,I Hilgert,H Kristofová,S Vanĕk,V Horejsí

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-CD18 antibody [MEM-48]

Flow Cytometry - Biotin Anti-CD18 antibody [MEM-48] (AB28093)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Epitope

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise