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AB51819

Biotin Anti-CD209B/DC-SIGNR1 antibody [ER-TR9]

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(5 Publications)

Rat Monoclonal C209A antibody ER-TR9. Suitable for IHC-Fr and reacts with Mouse samples. Cited in 5 publications.

View Alternative Names

CD209, Cire, Cd209a, CD209 antigen-like protein A, Dendritic cell-specific ICAM-3-grabbing non-integrin, DC-SIGN

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

ER-TR9

Isotype

IgM

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Reacts with

Mouse

Applications

IHC-Fr

applications

Immunogen

Tissue preparation containing Cd209a protein. The exact immunogen used to generate this antibody is proprietary information.

Q91ZX1

Epitope

The antigen is a glutaraldehyde (0.05%) resistant protein expressed in the cytoplasm and on the cell surface.

Specificity

Reacts with subpopulation of mature tissue macrophages present in the splenic marginal zone, lymph node medullary and trabecular sinuses.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

The antigen recognized by ER-TR9 has recently been shown to be the murine analogue of the human DC SIGN (Dendritic Cell - Specific ICAM3 Grabbing Non Integrin), named SIGN R1. Antigen Distribution: Isolated Cells: The antigen is found on a subpopulation of phagocytic macrophages isolated from the spleen and showing acid phosphatase and moderate non-specific esterase activity. These phagocytes selectively ingest neutral polysaccharides such as Ficoll. Tissue Sections: Subpopulation of resident macrophages in the splenic marginal zone which are in the proximity of a certain B cell subpopulation (μ+, d-). It is also found on a subpopulation of macrophages localized in the medullary sinuses and trabecular sinuses of lymph nodes. Furthermore, macrophage subpopulations in other organs, such as some connective tissue macrophages in the dermis, may also show ER-TR9 antigen expression.

Monoclonal antibody ER-TR9 is a very useful marker for the identification of macrophage subpopulations present in the marginal zone of spleen and lymph node medulla. ER-TR9 is also useful when studying phagocytosis of polysaccharides since the antibody selectively inhibits uptake of these glycans by macrophages.

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Properties and storage information

Form
Liquid
Purity
IgM fraction
Storage buffer
pH: 7.2 Preservative: 0.05% KATHON™ CG/ICP Constituents: 99% PBS, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD209B also known as DC-SIGNR1 is a C-type lectin receptor with a molecular mass of about 45 kDa. This receptor is similar in structure to CD209A another member of the same gene family. CD209B is mainly expressed on macrophages and dendritic cells which are critical components of the immune system. This receptor plays a role in the immune response by recognizing and binding to specific carbohydrate structures on the surface of various pathogens.
Biological function summary

CD209B functions as part of a broader set of protein interactions involved in pathogen recognition and clearance. It acts as a pattern recognition receptor and is involved in initiating immune responses. Although not part of a large complex CD209B shares functional similarities with other receptors like CD209A mediating defensive strategies against infections through binding to glycoprotein antigens on microbes.

Pathways

The receptor participates in the immune signaling networks that oversee pathogen recognition. It is vital within the innate immune pathway where it helps to stimulate adaptive immunity. CD209B engages with proteins such as Toll-like receptors which amplify immune responses against pathogens.

CD209B has potential implications in infectious diseases and autoimmune disorders. Its ability to bind diverse pathogens makes it an important player in infections like HIV where it helps the virus gain entry into host cells. Additionally its interaction with proteins like mannose receptor suggests a role in diseases characterized by chronic inflammation. Understanding these interactions assists in developing interventions to modulate immune response in such conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probable pathogen-recognition receptor. May mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. May recognize in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens.
See full target information Cd209a
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

ACS nano 14:10168-10186 PubMed32658456

2020

Splenic Capture and Intracellular Biodegradation of Biological-Grade Graphene Oxide Sheets.

Applications

Unspecified application

Species

Unspecified reactive species

Leon Newman,Dhifaf A Jasim,Eric Prestat,Neus Lozano,Irene de Lazaro,Yein Nam,Bakri M Assas,Joanne Pennock,Sarah J Haigh,Cyrill Bussy,Kostas Kostarelos

International immunology 15:177-86 PubMed12578847

2003

SIGN-R1, a novel C-type lectin expressed by marginal zone macrophages in spleen, mediates uptake of the polysaccharide dextran.

Applications

Unspecified application

Species

Unspecified reactive species

Young-Sun Kang,Sayuri Yamazaki,Tomonori Iyoda,Maggie Pack,Sandra A Bruening,Jae Y Kim,Kazuhiko Takahara,Kayo Inaba,Ralph M Steinman,Chae Gyu Park

European journal of immunology 19:675-80 PubMed2471648

1989

Marginal zone macrophages and their role in the immune response against T-independent type 2 antigens: modulation of the cells with specific antibody.

Applications

Unspecified application

Species

Unspecified reactive species

G Kraal,H Ter Hart,C Meelhuizen,G Venneker,E Claassen

Immunology 55:23-30 PubMed3888828

1985

Marginal zone macrophages identified by a monoclonal antibody: characterization of immuno- and enzyme-histochemical properties and functional capacities.

Applications

Unspecified application

Species

Unspecified reactive species

C D Dijkstra,E Van Vliet,E A Döpp,A A van der Lelij,G Kraal

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 33:40-4 PubMed3880783

1985

Marginal zone macrophages in the mouse spleen identified by a monoclonal antibody. Anatomical correlation with a B cell subpopulation.

Applications

Unspecified application

Species

Unspecified reactive species

E van Vliet,M Melis,W van Ewijk
View all publications
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