JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB33393

Biotin Anti-CD226 antibody [DX11]

Be the first to review this product! Submit a review

|

(1 Publication)

Mouse Monoclonal CD226 antibody - conjugated to Biotin. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing CD226 protein.

View Alternative Names

CD226, DNAM1, CD226 antigen, DNAX accessory molecule 1, DNAM-1

  • Unconjugated

    Anti-CD226 antibody [DX11]

  • 519 FITC

    FITC Anti-CD226 antibody [DX11]

  • 578 PE

    PE Anti-CD226 antibody [DX11]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

DX11

Isotype

IgG1

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing CD226 protein. The exact immunogen used to generate this antibody is proprietary information.

Q15762

style
left-column

Alternative Products

Primary Antibodies

AB61790

Anti-CD226 (phospho S329) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD226 (phospho S329) antibody (AB61790)

  • 5
  • 1
Primary Antibodies

AB245186

Anti-CD226 antibody [EPR21245]

Recombinant

Flow Cytometry - Anti-CD226 antibody [EPR21245] (AB245186)

  • 0
  • 0
Primary Antibodies

AB212077

Anti-CD226 antibody [EPR20710]

RabMAb|Recombinant|20ul selling size|Trial Size|Trial Size|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD226 antibody [EPR20710] (AB212077)

  • 0
  • 0
Primary Antibodies

AB230865

Anti-CD226 antibody [EPR21172] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD226 antibody [EPR21172] - BSA and Azide free (AB230865)

  • 0
  • 0
Primary Antibodies

AB212076

Anti-CD226 antibody [EPR21172]

RabMAb|Recombinant|Trial Size|Trial Size|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD226 antibody [EPR21172] (AB212076)

  • 0
  • 0

Complementary Products

Assay Kits

AB282303

Human CD226 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CD226 ELISA Kit (AB282303)

  • 0
  • 0
Primary Antibodies

AB91357

PE Mouse IgG1 [B11/6] - Isotype Control

  • 0
  • 0
Primary Antibodies

AB282789

PE Anti-CD73 antibody [EPR23048-139]

RabMAb|Recombinant

Flow Cytometry - PE Anti-CD73 antibody [EPR23048-139] (AB282789)

  • 0
  • 0
Primary Antibodies

AB223930

PE Anti-CD34 antibody [EP373Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - PE Anti-CD34 antibody [EP373Y] (AB223930)

  • 5
  • 1
Primary Antibodies

AB306574

Alexa Fluor® 647 Anti-CD33 antibody[EPR23052-5]

RabMAb|Recombinant

Flow Cytometry - Alexa Fluor® 647 Anti-CD33 antibody[EPR23052-5] (AB306574)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1/1 - 1/10", "FlowCyt-species-notes": "<p>Use 10μl of the suggested working dilution to label 1 x 10<sup>6</sup> cells in 100μl.</p>" } } }
style
left-column

Product details

This antibody has been reported to inhibit T- and NK cell mediated cytotoxicity against tumour cell targets and to block TNF alpha and IFN gamma secretion by alloantigen-specific T-cells.
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD226 also known as DNAM-1 (DNAX Accessory Molecule-1) is a transmembrane protein expressed on the surface of various immune cells such as T cells natural killer (NK) cells and some B cells. The protein has a molecular weight of approximately 65 kDa. CD226 is part of the Ig superfamily and plays a role in the adhesion and signaling processes of immune cells. It interacts with its ligands CD112 (PVRL2) and CD155 (PVR) on target cells facilitating immune synapse formation and cytotoxic activity.
Biological function summary

CD226 is involved in immune cell activation and receptor-mediated signaling. It aids the recognition and destruction of target cells by NK cells and cytotoxic T lymphocytes through its interaction with ligand-expressing cells. CD226 does not work alone but functions as part of a complex system of co-stimulatory and inhibitory receptors balancing immune responses. Its cooperation with receptors such as CD96 and TIGIT (T cell immunoreceptor with Ig and ITIM domains) modulates the activity of immune effector cells.

Pathways

CD226 takes part in immune signaling pathways that regulate cell-mediated cytotoxicity and inflammation. It is an important component of the immune checkpoint axis interacting closely with proteins like CD96 and TIGIT within these pathways. This interaction helps determine the fate of immune responses influencing the progression or resolution of inflammatory and autoimmune conditions. CD226 plays a role in the NK cell-mediated cytotoxicity pathway and the adaptive immune response.

CD226 has been linked to autoimmune diseases such as multiple sclerosis and type 1 diabetes. Abnormal expression or function of CD226 can influence the pathogenesis of these conditions often through interactions with related proteins like TIGIT impacting immune tolerance and effector cell function. CD226's involvement in regulating immune responses highlights its importance as a potential therapeutic target in managing autoimmune and other immune-related disorders.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Cell surface receptor that plays an important role in the immune system, particularly in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-cells and NK cells (PubMed : 8673704, PubMed : 9712030). Functions as a costimulatory receptor upon recognition of target cells, such as virus-infected or tumor cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112 on target cells, promotes the cytotoxic activity of NK cells and CTLs, enhancing their ability to kill these cells (PubMed : 26755705, PubMed : 31253644, PubMed : 30591568). Mechanistically, phosphorylation by Src kinases such as LYN of FYN, enables binding to adapter GRB2, leading to activation of VAV1, PI3K and PLCG1. Promotes also activation of kinases ERK and AKT, as well as calcium fluxes (By similarity).
See full target information CD226
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science 118:4577-86 PubMed16179614

2005

TGF-beta-induced apoptosis in endothelial cells mediated by M6P/IGFII-R and mini-plasminogen.

Applications

Unspecified application

Species

Unspecified reactive species

Vladimír Leksa,Samuel Godar,Herbert B Schiller,Elke Fuertbauer,Arshad Muhammad,Katarina Slezakova,Vaclav Horejsi,Peter Steinlein,Ulrich H Weidle,Bernd R Binder,Hannes Stockinger
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com