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AB191112

Biotin Anti-CD3 antibody [UCHT1]

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(2 Publications)

Mouse Monoclonal CD3d antibody - conjugated to Biotin. Suitable for Flow Cyt and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing CD3D protein.

View Alternative Names

CD3d, T3D, CD3D, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain

2 Images
Flow Cytometry - Biotin Anti-CD3 antibody [UCHT1] (AB191112)
  • Flow Cyt

Supplier Data

Flow Cytometry - Biotin Anti-CD3 antibody [UCHT1] (AB191112)

Separation of human CD3 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab191112 (concentration in sample 0.3 µg/mL) streptavidin APC.

Flow Cytometry - Biotin Anti-CD3 antibody [UCHT1] (AB191112)
  • Flow Cyt

Supplier Data

Flow Cytometry - Biotin Anti-CD3 antibody [UCHT1] (AB191112)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab191112 (concentration in sample 0.3 µg/mL) streptavidin APC.

  • 660 APC

    APC Anti-CD3 antibody [UCHT1]

  • 785 APC/Cy7®

    APC/Cy7® Anti-CD3 antibody [UCHT1]

  • 519 FITC

    FITC Anti-CD3 antibody [UCHT1]

  • 519 FITC

    FITC Anti-CD3 epsilon antibody [UCHT1]

  • 578 PE

    PE Anti-CD3 epsilon antibody [UCHT1]

  • 667 PE/Cy5®

    PE/Cy5® Anti-CD3 antibody [UCHT1]

  • 778 PE/Cy7®

    PE/Cy7® Anti-CD3 antibody [UCHT1]

  • 675 PerCP

    PerCP Anti-CD3 antibody [UCHT1]

  • 695 PerCP/Cy5.5®

    PerCP/Cy5.5® Anti-CD3 antibody [UCHT1]

  • 387 mFluor™ UV375

    mFluor™ UV375 Anti-CD3e antibody [UCHT1]

  • 454 mFluor™ Violet 450

    mFluor™ Violet 450 Anti-CD3e antibody [UCHT1]

  • 704 redFluor™ 710

    redFluor™ 710 Anti-CD3 antibody [UCHT1]

  • 450 violetFluor™ 450

    violetFluor™ 450 Anti-CD3 antibody [UCHT1]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

UCHT1

Isotype

IgG1

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing CD3D protein. The exact immunogen used to generate this antibody is proprietary information.

P04234

Specificity

ab191112 recognizes the CD3 antigen of the TCR/CD3 complex on mature Human T cells. The antibody reacts with the epsilon chain of the CD3 complex.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-12 µg/mL", "FlowCyt-species-notes": "<p></p>" }, "Primates": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Purification notes
ab191112 is free of unconjugated biotin.
Storage buffer
pH: 7.4 Preservative: 0.0975% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells (PubMed : 12215456).
See full target information CD3D
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Biosensors 12: PubMed35049640

2021

Development of a Microfluidic Device for CD4 T Cell Isolation and Automated Enumeration from Whole Blood.

Applications

Unspecified application

Species

Unspecified reactive species

Robert D Fennell,Mazhar Sher,Waseem Asghar

The Journal of experimental medicine 178:1121-6 PubMed7688788

1993

A novel 80-kD cell surface structure identifies human circulating lymphocytes with natural killer activity.

Applications

Unspecified application

Species

Unspecified reactive species

H Maïza,G Leca,I G Mansur,V Schiavon,L Boumsell,A Bensussan
View all publications
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Product promise

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