AB30355

Biotin Anti-CD32B + CD32A antibody [AT10]

Name: Anti-CD32B + CD32A Biotin antibody [AT10] (ab30355) IgG1 | Abcam
Brand: Abcam Limited
SKU: AB30355
Price: 270 USD
Availability: InStock

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(4 Publications)

Mouse Monoclonal FCG2B antibody - conjugated to Biotin. Suitable for Flow Cyt and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Cell preparation containing FCGR2B protein.

View Alternative Names

CD32, FCG2, IGFR2, FCGR2B, Low affinity immunoglobulin gamma Fc region receptor II-b, IgG Fc receptor II-b, CDw32, Fc-gamma RII-b, Fc-gamma-RIIb, FcRII-b

1 Images

Flow Cytometry - Biotin Anti-CD32B + CD32A antibody [AT10] (AB30355)

Flow Cyt

Unknown

Flow Cytometry - Biotin Anti-CD32B + CD32A antibody [AT10] (AB30355)

Flow cytometric analysis of human peripheral blood monocytes with 10μl for 10⁶ cells of anti human CD32A + CD32B Biotin conjugated (ab30355).

519 FITC

FITC Anti-CD32B + CD32A antibody [AT10]
Unconjugated

Anti-CD32B + CD32A antibody [AT10] - BSA free
578 PE

PE Anti-CD32B + CD32A antibody [AT10]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

AT10

Isotype

IgG1

Conjugation

Biotin

Excitation/Emission

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing FCGR2B protein. The exact immunogen used to generate this antibody is proprietary information.

P31994

Specificity

According to the CD32A + CD32B Cluster workshop (Leucocyte Typing V, pp823, pp828), clone AT10 reacts equally well with both Fc gamma RII a and b-expressing cells.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/biotin-mouse-igg1-kappa-monoclonal-mopc-21-isotype-control-ab18434'>ab18434</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis. Isoform IIB2 does not trigger phagocytosis.

See full target information FCGR2B

Additional targets

FCGR2A

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of biomedical science 22:87 PubMed26475492

2015

FcγRIIB mediates antigen-independent inhibition on human B lymphocytes through Btk and p38 MAPK.

Applications

Unspecified application

Species

Unspecified reactive species

Shiang-Jong Tzeng,Wan-Yu Li,Hui-Ying Wang

Veterinary immunology and immunopathology 61:181-93 PubMed9613433

1998

Expression of adhesion and Fcgamma-receptors on canine blood eosinophils and neutrophils studied by anti-human monoclonal antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

I Lilliehöök,A Johannisson,L Håkansson

Blood 91:2369-80 PubMed9516136

1998

Expression of functional CD32 molecules on human NK cells is determined by an allelic polymorphism of the FcgammaRIIC gene.

Applications

Unspecified application

Species

Unspecified reactive species

D Metes,L K Ernst,W H Chambers,A Sulica,R B Herberman,P A Morel

Molecular immunology 28:1243-54 PubMed1835758

1991

Characterization of a new monoclonal anti-Fc gamma RII antibody, AT10, and its incorporation into a bispecific F(ab')2 derivative for recruitment of cytotoxic effectors.

Applications

Unspecified application

Species

Unspecified reactive species

J Greenman,A L Tutt,A J George,K A Pulford,G T Stevenson,M J Glennie

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-CD32B + CD32A antibody [AT10]

Flow Cytometry - Biotin Anti-CD32B + CD32A antibody [AT10] (AB30355)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Additional targets

Publications (4)

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