Mouse Monoclonal CD33 antibody - conjugated to Biotin. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Cell preparation containing CD33 protein.
Images
![Flow Cytometry - Biotin Anti-CD33 antibody [HIM3-4] (AB21892), expandable thumbnail](https://content.abcam.com/products/images/biotin-cd33-antibody-him3-4-ab21892--flow-cytometry-img81798.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Biotin Anti-CD33 antibody [HIM3-4] (AB21892)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- Biotin
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Cell preparation containing CD33 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P20138
Reactivity data
Select an application| Flow Cyt | |
|---|---|
| Human | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-12.00000 µg/mL | Notes ab18434 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Associated Products
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Target data
Function
Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state (PubMed:10611343, PubMed:11320212, PubMed:15597323). Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans (PubMed:7718872). Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK (PubMed:10887109, PubMed:28325905). These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:10206955, PubMed:10556798, PubMed:10887109). In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules (PubMed:10206955, PubMed:10887109). One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K (PubMed:15597323).
Alternative names
CD33, SIGLEC3, Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, gp67, Siglec-3
Recommended products
Mouse Monoclonal CD33 antibody - conjugated to Biotin. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Cell preparation containing CD33 protein.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- Biotin
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Cell preparation containing CD33 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P20138
- Clone number
- HIM3-4
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
antibody purified.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
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Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Flow Cytometry - Biotin Anti-CD33 antibody [HIM3-4] (ab21892), expandable thumbnail](https://content.abcam.com/products/images/biotin-cd33-antibody-him3-4-ab21892--flow-cytometry-img81798.jpg "Flow Cytometry - Biotin Anti-CD33 antibody [HIM3-4] (ab21892)")
Flow Cytometry - Biotin Anti-CD33 antibody [HIM3-4] (ab21892)
Flow Cytometry analysis of human peripheral blood cells labeling CD33 with Anti-CD33 antibody [HIM3-4] (Biotin) (ab21892).
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