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AB21892

Biotin Anti-CD33 antibody [HIM3-4]

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(1 Publication)

Mouse Monoclonal CD33 antibody - conjugated to Biotin. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing CD33 protein.

View Alternative Names

CD33, SIGLEC3, Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, gp67, Siglec-3

1 Images
Flow Cytometry - Biotin Anti-CD33 antibody [HIM3-4] (AB21892)
  • Flow Cyt

Unknown

Flow Cytometry - Biotin Anti-CD33 antibody [HIM3-4] (AB21892)

Flow Cytometry analysis of human peripheral blood cells labeling CD33 with Anti-CD33 antibody [HIM3-4] (Biotin) (ab21892).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HIM3-4

Isotype

IgG1

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing CD33 protein. The exact immunogen used to generate this antibody is proprietary information.

P20138

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-12 µg/mL", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/biotin-mouse-igg1-kappa-monoclonal-mopc-21-isotype-control-ab18434'>ab18434</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
antibody purified.
Storage buffer
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state (PubMed : 10611343, PubMed : 11320212, PubMed : 15597323). Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans (PubMed : 7718872). Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK (PubMed : 10887109, PubMed : 28325905). These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed : 10206955, PubMed : 10556798, PubMed : 10887109). In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules (PubMed : 10206955, PubMed : 10887109). One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K (PubMed : 15597323).
See full target information CD33
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Plant physiology and biochemistry : PPB 140:78-87 PubMed31085449

2019

Heat stress reveals high molecular mass proteasomes in Arabidopsis thaliana suspension cells cultures.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Aristizábal,Viridiana Rivas,Gladys I Cassab,Fernando Lledías
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