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AB28104

Biotin Anti-CD43 antibody [MEM-59]

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(1 Publication)

Mouse Monoclonal CD43 antibody - conjugated to Biotin. Suitable for IP, Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing SPN protein.

View Alternative Names

CD43, SPN, Leukosialin, GPL115, Galactoglycoprotein, Leukocyte sialoglycoprotein, Sialophorin, GALGP

2 Images
Flow Cytometry - Biotin Anti-CD43 antibody [MEM-59] (AB28104)
  • Flow Cyt

Supplier Data

Flow Cytometry - Biotin Anti-CD43 antibody [MEM-59] (AB28104)

Separation of human CD43 positive lymphocytes (red-filled) from CD43 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab28104 (concentration in sample 0.56 µg/ml, streptavidin APC).

Flow Cytometry - Biotin Anti-CD43 antibody [MEM-59] (AB28104)
  • Flow Cyt

Supplier Data

Flow Cytometry - Biotin Anti-CD43 antibody [MEM-59] (AB28104)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab28104 (concentration in sample 0.56 µg/ml, streptavidin APC).

  • Unconjugated

    Anti-CD43 antibody [MEM-59]

  • 660 APC

    APC Anti-CD43 antibody [MEM-59]

  • 578 PE

    PE Anti-CD43 antibody [MEM-59]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-59

Isotype

IgG1

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Reacts with

Human

Applications

IHC-P, IP, WB, Flow Cyt

applications

Immunogen

Cell preparation containing SPN protein. The exact immunogen used to generate this antibody is proprietary information.

P16150

Specificity

The antibody recognizes neuraminidase sensitive epitope on CD43 antigen (Leukosialin), which is involved in lymphocyte activation.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µg/mL", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/biotin-mouse-igg1-kappa-monoclonal-mopc-21-isotype-control-ab18434'>ab18434</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Purification notes
Purity >95% by SDS-PAGE.
Storage buffer
pH: 8 Preservative: 0.097% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration. Positively regulates T-cell trafficking to lymph-nodes via its association with ERM proteins (EZR, RDX and MSN) (By similarity). Negatively regulates Th2 cell differentiation and predisposes the differentiation of T-cells towards a Th1 lineage commitment. Promotes the expression of IFN-gamma by T-cells during T-cell receptor (TCR) activation of naive cells and induces the expression of IFN-gamma by CD4(+) T-cells and to a lesser extent by CD8(+) T-cells (PubMed : 18036228). Plays a role in preparing T-cells for cytokine sensing and differentiation into effector cells by inducing the expression of cytokine receptors IFNGR and IL4R, promoting IFNGR and IL4R signaling and by mediating the clustering of IFNGR with TCR (PubMed : 24328034). Acts as a major E-selectin ligand responsible for Th17 cell rolling on activated vasculature and recruitment during inflammation. Mediates Th17 cells, but not Th1 cells, adhesion to E-selectin. Acts as a T-cell counter-receptor for SIGLEC1 (By similarity).. CD43 cytoplasmic tail. Protects cells from apoptotic signals, promoting cell survival.
See full target information SPN
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

European journal of immunology 25:1051-5 PubMed7537669

1995

MEM-59 monoclonal antibody detects a CD43 epitope involved in lymphocyte activation.

Applications

Unspecified application

Species

Unspecified reactive species

M Alvarado,C Klassen,J Cerny,V Horejsí,R E Schmidt
View all publications
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Product promise

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