AB28107

Biotin Anti-CD45 antibody [MEM-28]

Name: Anti-CD45 Biotin antibody [MEM-28] (ab28107) Mouse monoclonal IgG1 | Abcam
Brand: Abcam Limited
SKU: AB28107
Price: 525 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal CD45 antibody - conjugated to Biotin. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing PTPRC protein.

View Alternative Names

CD45, PTPRC, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, L-CA

2 Images

Flow Cytometry - Biotin Anti-CD45 antibody [MEM-28] (AB28107)

Flow Cyt

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Flow Cytometry - Biotin Anti-CD45 antibody [MEM-28] (AB28107)

Flow Cytometry analysis of Human peripheral blood cells labeling CD45 with Anti-CD45 antibody [MEM-28] (Biotin) (ab28107).

Supplier Data

Western blot - Biotin Anti-CD45 antibody [MEM-28] (AB28107)

Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Jurkat, Raji, HeLa, and HEK293T cell lines, mixed and heated (100°C, 5 min) with non-reducing SDS-loading buffer. Samples were resolved using 7% Tris-glycine SDS gel electrophoresis. Nitrocellulose membrane blot was probed with ab28107 (1 µg/ml), followed by IRDye 800CW Streptavidin (green). Multiplex fluorescent Western blot detection was performed. CD45 molecules were detected at ~180-250 kDa in Jurkat and Raji cell lines

All lanes:

Western blot - Biotin Anti-CD45 antibody [MEM-28] (ab28107)

Lanes 1 and 4:

Jurkat whole cell extract with non-reducing SDS-loading buffer

Lane 2:

Raji whole cell extract with non-reducing SDS-loading buffer

Lane 3:

HeLa whole cell extract with non-reducing SDS-loading buffer

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Unconjugated

Anti-CD45 antibody [MEM-28]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-CD45 antibody [MEM-28]
660 APC

APC Anti-CD45 antibody [MEM-28]
675 PerCP

PerCP Anti-CD45 antibody [MEM-28]
778 PE/Cy7®

PE/Cy7® Anti-CD45 antibody [MEM-28]
785 APC/Cy7®

APC/Cy7® Anti-CD45 antibody [MEM-28]
618 PE/DyLight™ 594

PE/DyLight™ 594 Anti-CD45 antibody [MEM-28]
519 FITC

FITC Anti-CD45 antibody [MEM-28]
695 PerCP/Cy5.5®

PerCP/Cy5.5® Anti-CD45 antibody [MEM-28]
578 PE

PE Anti-CD45 antibody [MEM-28]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-28

Isotype

IgG1

Light chain type

kappa

Conjugation

Biotin

Excitation/Emission

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing PTPRC protein. The exact immunogen used to generate this antibody is proprietary information.

P08575

Specificity

Ab28107 reacts with all alternative forms of CD45 antigen expressed at high level on all cells of hematopoetic origin, except erythrocytes and platelets.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/biotin-mouse-igg1-kappa-monoclonal-mopc-21-isotype-control-ab18434'>ab18434</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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Product details

The antibody is conjugated with Biotin LC NHS under optimum conditions. The reagent is free of unconjugated biotin.

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Purification notes

Purity >95% by SDS-PAGE.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD45 also known as Protein tyrosine phosphatase receptor type C (PTPRC) is a transmembrane glycoprotein with a molecular mass ranging between 180-240 kDa depending on its isoform. It is expressed on the surface of almost all hematopoietic cells except for mature erythrocytes and platelets. CD45 has a critical role in regulating antigen receptor signaling by modifying kinases involved in signal transduction and it is essential in lymphocyte development and activation. Because of its broad expression on immune cells CD45 is a valuable marker for differentiating various immune cell types in assays like flow cytometry and immunohistochemistry (IHC) often referred to as CD45 stain.

Biological function summary

CD45 acts by regulating tyrosine phosphorylation in the immune cell signaling context participating directly in signal transduction. It functions by dephosphorylating specific phosphotyrosine residues on various proteins modulating the signaling threshold required for lymphocyte activation. Although not known to be part of a protein complex CD45 itself shows isoform variation that associates with specific immune cell types impacting their function. Importantly CD45 interacts with multiple signaling molecules to affect cell growth and differentiation.

Pathways

Scientists associate CD45 with signaling pathways such as the T-cell receptor (TCR) and B-cell receptor (BCR) signaling. CD45 modulates the activity of Src family kinases important elements in these pathways making it essential for effective immune response and tolerance. Furthermore interactions between CD45 and proteins like Lck in T-cells or Lyn in B-cells highlight its pivotal role in executing its signaling functions.

Scientists often link CD45 to immune-related conditions including autoimmunity and leukemia. In autoimmune diseases altered CD45 expression or activity can disrupt normal immune function contributing to pathogenesis. In leukemia CD45 expression levels can assist in disease classification and prognosis as it interacts with proteins involved in cell cycle regulation. The anti-CD45 antibodies can provide diagnostic and therapeutic avenues highlighting their utility in disease management.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed : 35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.

See full target information PTPRC

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 277:7955-61 PubMed11773067

2002

Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules.

Applications

Unspecified application

Species

Unspecified reactive species

Lukás Cermák,Sárka Símová,Alexandros Pintzas,Vaclav Horejsí,Ladislav Andera

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-CD45 antibody [MEM-28]

Flow Cytometry - Biotin Anti-CD45 antibody [MEM-28] (AB28107)

Western blot - Biotin Anti-CD45 antibody [MEM-28] (AB28107)

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Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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