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AB28114

Biotin Anti-CD59 antibody [MEM-43]

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(3 Publications)

Mouse Monoclonal CD59 antibody - conjugated to Biotin. Suitable for Flow Cyt and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Cell preparation containing CD59 protein.

View Alternative Names

CD59, MIC11, MIN1, MIN2, MIN3, MSK21, CD59 glycoprotein, 1F5 antigen, 20 kDa homologous restriction factor, MAC-inhibitory protein, MEM43 antigen, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, Protectin, HRF-20, HRF20, MAC-IP, MACIF, MIRL

1 Images
Flow Cytometry - Biotin Anti-CD59 antibody [MEM-43] (AB28114)
  • Flow Cyt

Unknown

Flow Cytometry - Biotin Anti-CD59 antibody [MEM-43] (AB28114)

Flow Cytometry analysis of positive HL60 cells (human promyelocytic leukemia), and negative SP2 (murine myeloma) cells labeling CD59 with ab28114.

  • Unconjugated

    Anti-CD59 antibody [MEM-43]

  • 660 APC

    APC Anti-CD59 antibody [MEM-43]

  • 578 PE

    PE Anti-CD59 antibody [MEM-43]

  • 519 FITC

    FITC Anti-CD59 antibody [MEM-43]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CD59 antibody [MEM-43]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-43

Isotype

IgG2a

Light chain type

kappa

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing CD59 protein. The exact immunogen used to generate this antibody is proprietary information.

P13987

Epitope

ab28114 reacts with a well defined epitope (W40, R-53) on CD59 molecule.

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Alternative Products

Primary Antibodies

AB9183

Anti-CD59 antibody [MEM-43/5]

BOND RX™ Validated

Immunocytochemistry/ Immunofluorescence - Anti-CD59 antibody [MEM-43/5] (AB9183)

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Primary Antibodies

AB36421

PE Anti-CD59 antibody [MEM-43]

Immunocytochemistry/ Immunofluorescence - PE Anti-CD59 antibody [MEM-43] (AB36421)

  • 0
  • 0
Primary Antibodies

AB133707

Anti-CD59 antibody [EPR6425(2)]

RabMAb|Recombinant|KO Validated

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  • 0
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Primary Antibodies

AB124396

Anti-CD59 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody (AB124396)

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Complementary Products

Assay Kits

AB263893

Human CD59 ELISA Kit

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ELISA - Human CD59 ELISA Kit (AB263893)

  • 0
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Proteins & Peptides

AB134887

Recombinant Human CD59 protein

SDS-PAGE - Recombinant Human CD59 protein (AB134887)

  • 0
  • 0
Assay Kits

AB263893

Human CD59 ELISA Kit

Recombinant|SimpleStep

ELISA - Human CD59 ELISA Kit (AB263893)

  • 0
  • 0
Primary Antibodies

AB309977

HRP Anti-CD55 antibody [EPR6689]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-CD55 antibody [EPR6689] (AB309977)

  • 0
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Primary Antibodies

AB196147

Alexa Fluor® 647 Anti-CD4 antibody [EPR6855]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD4 antibody [EPR6855] (AB196147)

  • 5
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µg/mL", "FlowCyt-species-notes": "<p><a href='/en-us/products/unavailable/mouse-igg2a-monoclonal-biotin-isotype-control-ab97679'>ab97679</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

The antibody is conjugated with Biotin-LC-NHS and it is free of unconjugated biotin.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD59 also known as protectin is a protein with a molecular mass of approximately 18-25 kDa. It is widely expressed on the surface of many human cells including red blood cells and various types of leukocytes. CD59 serves as an inhibitor of the complement membrane attack complex (MAC) preventing cell lysis caused by terminal complement proteins. Through this mechanism it regulates complement activation and maintains cell integrity by halting the formation of MAC.
Biological function summary

One important feature of CD59 is its role as a glycosylphosphatidylinositol (GPI)-anchored protein involved in safeguarding cells from complement-mediated damage. It does not exist within a larger complex but functions at the cell surface to inhibit the assembly of complement components C5b-9 which form the MAC. This ability to inhibit MAC is essential in maintaining self-tissue from unintended damage during immune responses.

Pathways

CD59 participates in the regulation of the complement cascade specifically within the terminal pathway. The complement system serves as a bridge between innate and adaptive immunity contributing to processes like opsonization and cell lysis. CD59's inhibitory action directly impacts pathways that utilize terminal components such as C5b. Effective CD59 function prevents excessive complement activation ensuring that an immune response does not damage host tissues. It links closely with other complement regulatory proteins like CD55 which also mitigate complement cascade activation.

Deficiencies or malfunctions in CD59 have notable implications. Paroxysmal nocturnal hemoglobinuria (PNH) is a disorder where lack of CD59 expression on blood cells leads to their increased destruction due to unregulated complement activity. Furthermore CD59's dysfunction or inadequacy also associates with atypical hemolytic uremic syndrome (aHUS) which involves mutant complement regulatory proteins causing overactivation of the complement system. These associations highlight the importance of CD59 in both maintaining cellular health and preventing pathophysiological conditions related to complement overactivity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Potent inhibitor of the complement membrane attack complex (MAC) action, which protects human cells from damage during complement activation (PubMed : 11882685, PubMed : 1698710, PubMed : 2475111, PubMed : 2475570, PubMed : 2606909, PubMed : 9053451). Acts by binding to the beta-haipins of C8 (C8A and C8B) components of the assembling MAC, forming an intermolecular beta-sheet that prevents incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore (PubMed : 11882685, PubMed : 1698710, PubMed : 36797260).. The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit complement membrane attack complex (MAC) assembly on cell membranes.
See full target information CD59
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Biochemical and biophysical research communications 243:706-10 PubMed9500981

1998

Incorporation of leucocyte GPI-anchored proteins and protein tyrosine kinases into lipid-rich membrane domains of COS-7 cells.

Applications

Unspecified application

Species

Unspecified reactive species

M Cebecauer,J Cerný,V Horejsí

Science (New York, N.Y.) 254:1016-9 PubMed1719635

1991

GPI-anchored cell-surface molecules complexed to protein tyrosine kinases.

Applications

Unspecified application

Species

Unspecified reactive species

I Stefanová,V Horejsí,I J Ansotegui,W Knapp,H Stockinger

Molecular immunology 26:153-61 PubMed2918859

1989

Characterization of a broadly expressed human leucocyte surface antigen MEM-43 anchored in membrane through phosphatidylinositol.

Applications

Unspecified application

Species

Unspecified reactive species

I Stefanová,I Hilgert,H Kristofová,R Brown,M G Low,V Horejsí
View all publications
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