AB134331

Biotin Anti-CD63 antibody [MEM-259]

Name: Biotin Anti-CD63 antibody [MEM-259] (ab134331) | Abcam
Brand: Abcam Limited
SKU: AB134331
Price: 435 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(8 Publications)

Mouse Monoclonal CD63 antibody - conjugated to Biotin. Suitable for Flow Cyt and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Cell preparation containing CD63 protein.

View Alternative Names

CD63, MLA1, TSPAN30, CD63 antigen, Granulophysin, Lysosomal-associated membrane protein 3, Lysosome integral membrane protein 1, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, LAMP-3, Limp1, Tspan-30

3 Images

Flow Cytometry - Biotin Anti-CD63 antibody [MEM-259] (AB134331)

Flow Cyt

Supplier Data

Flow Cytometry - Biotin Anti-CD63 antibody [MEM-259] (AB134331)

Flow cytometry multicolor surface staining of IgE stimulated peripheral blood mononuclear cells stained usingab134331 (concentration in sample 0.6 μg/mL, Streptavidin APC).

Flow Cyt

Supplier Data

Flow Cytometry - Biotin Anti-CD63 antibody [MEM-259] (AB134331)

Flow cytometry multicolor surface staining of IgE stimulated peripheral blood mononuclear cells stained usingab134331 (concentration in sample 0.6 μg/mL, Streptavidin APC) and anti-human CD203c PE antibody (20 μL reagent / 100 μL of peripheral whole blood).

Flow Cyt

Supplier Data

Flow Cytometry - Biotin Anti-CD63 antibody [MEM-259] (AB134331)

Flow Cytometry analysis of (surface staining) of IgE stimulated human peripheral whole blood using ab134331 (concentration in sample 0.6 μg/mL, Streptavidin APC). CD63 positive CD203c positive basophil granulocytes (red-filled) from neutrophil granulocytes (black-dashed).

Unconjugated

Anti-CD63 antibody [MEM-259]
578 PE

PE Anti-CD63 antibody [MEM-259]
667 PE/Cy5®

PE/Cy5® Anti-CD63 antibody [MEM-259]
675 PerCP

PerCP Anti-CD63 antibody [MEM-259]
519 FITC

FITC Anti-CD63 antibody [MEM-259]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-259

Isotype

IgG1

Conjugation

Biotin

Excitation/Emission

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing CD63 protein. The exact immunogen used to generate this antibody is proprietary information.

P08962

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }

style

left-column

Product details

The antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD63 is a protein commonly known as LAMP-3 a member of the tetraspanin family with a molecular weight of approximately 25-65 kDa depending on post-translational modifications such as glycosylation. It resides mainly on the membrane of intracellular vesicles such as lysosomes and platelets. CD63 also appears on the surface of activated cells particularly in immune cells. Researchers often use CD63 as a marker in assays including CD63 western blot techniques due to its distinct expression patterns.

Biological function summary

CD63 participates in various cellular processes including membrane trafficking cell adhesion and signal transduction. It associates with integrins and other tetraspanins forming complexes that influence cellular adhesion and migration. Through its role in exosome formation CD63 contributes significantly to intercellular communication by facilitating the transfer of proteins lipids and RNA between cells.

Pathways

CD63 plays a role in pathways linked to cell proliferation and migration and immune response regulation. Within the integrin-mediated signaling pathway CD63 interacts with proteins such as integrins to promote cell adhesion and migration. CD63 also links to vesicle trafficking pathways collaborating with proteins involved in exocytosis and endocytosis which affect cell membrane restructuring and intracellular transport.

CD63 shows connections with cancer and immune system disorders. Its role in promoting cell adhesion and migration links it with cancer metastasis including melanoma and breast cancer where CD63 expression levels often rise. Additionally CD63 associates with allergic reactions due to its involvement in histamine release where it impacts immune proteins like FcεRI which participate in allergy pathogenesis.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Functions as a cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli.

See full target information CD63

style

left-column

Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:17707 PubMed37853206

2023

Use of transcranial low-intensity focused ultrasound for targeted delivery of stem cell-derived exosomes to the brain.

Applications

Unspecified application

Species

Unspecified reactive species

J Haroon,K Aboody,L Flores,M McDonald,K Mahdavi,M Zielinski,K Jordan,E Rindner,J Surya,V Venkatraman,V Go-Stevens,G Ngai,J Lara,C Hyde,S Schafer,M Schafer,A Bystritsky,I Nardi,T Kuhn,D Ross,S Jordan

Journal of nanobiotechnology 20:530 PubMed36514065

2022

Antenna-enhanced mid-infrared detection of extracellular vesicles derived from human cancer cell cultures.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Eleonora Temperini,Flavio Di Giacinto,Sabrina Romanò,Riccardo Di Santo,Alberto Augello,Raffaella Polito,Leonetta Baldassarre,Valeria Giliberti,Massimiliano Papi,Umberto Basile,Benedetta Niccolini,Ewa K Krasnowska,Annalucia Serafino,Marco De Spirito,Alessandra Di Gaspare,Michele Ortolani,Gabriele Ciasca

Cell & bioscience 11:37 PubMed33568197

2021

Comparative evaluation of methods for isolating small extracellular vesicles derived from pancreatic cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jie-Min Wang,Yong-Jiang Li,Jun-Yong Wu,Jia-Xin Cai,Jing Wen,Da-Xiong Xiang,Xiong-Bin Hu,Wen-Qun Li

ACS sensors 5:764-771 PubMed32134252

2020

Enabling Sensitive Phenotypic Profiling of Cancer-Derived Small Extracellular Vesicles Using Surface-Enhanced Raman Spectroscopy Nanotags.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhang,Lianmei Jiang,Russell J Diefenbach,Douglas H Campbell,Bradley J Walsh,Nicolle H Packer,Yuling Wang

The Journal of clinical investigation 130:1185-1198 PubMed31770109

2019

CD73 immune checkpoint defines regulatory NK cells within the tumor microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Shi Yong Neo,Ying Yang,Julien Record,Ran Ma,Xinsong Chen,Ziqing Chen,Nicholas P Tobin,Emily Blake,Christina Seitz,Ron Thomas,Arnika Kathleen Wagner,John Andersson,Jana de Boniface,Jonas Bergh,Shannon Murray,Evren Alici,Richard Childs,Martin Johansson,Lisa S Westerberg,Felix Haglund,Johan Hartman,Andreas Lundqvist

The Analyst 144:5856-5865 PubMed31482867

2019

Exosome-specific tumor diagnosis via biomedical analysis of exosome-containing microRNA biomarkers.

Applications

Unspecified application

Species

Unspecified reactive species

Zhijin Fan,Jun Yu,Jingyan Lin,Ying Liu,Yuhui Liao

Nanoscale 10:20289-20295 PubMed30371719

2018

A dual-signal amplification platform for sensitive fluorescence biosensing of leukemia-derived exosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Huang,Dian-Bing Wang,Netrapal Singh,Fang Yang,Ning Gu,Xian-En Zhang

Neurobiology of disease 106:222-234 PubMed28610892

2017

Novel human neuronal tau model exhibiting neurofibrillary tangles and transcellular propagation.

Applications

Unspecified application

Species

Unspecified reactive species

Patrick Reilly,Charisse N Winston,Kelsey R Baron,Margarita Trejo,Edward M Rockenstein,Johnny C Akers,Najla Kfoury,Marc Diamond,Eliezer Masliah,Robert A Rissman,Shauna H Yuan

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-CD63 antibody [MEM-259]

Flow Cytometry - Biotin Anti-CD63 antibody [MEM-259] (AB134331)

Flow Cytometry - Biotin Anti-CD63 antibody [MEM-259] (AB134331)

Flow Cytometry - Biotin Anti-CD63 antibody [MEM-259] (AB134331)

Related conjugates and formulations (5)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise