AB28094

Biotin Anti-CD9 antibody [MEM-61]

Name: Anti-CD9 Biotin antibody [MEM-61] (ab28094) Mouse monoclonal IgG1 | Abcam
Brand: Abcam Limited
SKU: AB28094
Price: 430 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(3 Publications)

Mouse Monoclonal CD9 antibody - conjugated to Biotin. Suitable for Flow Cyt and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Cell preparation containing CD9 protein.

View Alternative Names

CD9, MIC3, TSPAN29, GIG2, CD9 antigen, 5H9 antigen, Cell growth-inhibiting gene 2 protein, Leukocyte antigen MIC3, Motility-related protein, Tetraspanin-29, p24, MRP-1, Tspan-29

2 Images

Flow Cytometry - Biotin Anti-CD9 antibody [MEM-61] (AB28094)

Flow Cyt

Supplier Data

Flow Cytometry - Biotin Anti-CD9 antibody [MEM-61] (AB28094)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab28094 (concentration in sample 1 µg/ml, Streptavidin APC).

Flow Cyt

Supplier Data

Flow Cytometry - Biotin Anti-CD9 antibody [MEM-61] (AB28094)

Separation of human CD9 positive CD45 negative platelets (red-filled) from CD9 negative CD45 positive lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab28094 (concentration in sample 1 µg/ml, Streptavidin APC).

Unconjugated

Anti-CD9 antibody [MEM-61]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-61

Isotype

IgG1

Light chain type

kappa

Conjugation

Biotin

Excitation/Emission

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing CD9 protein. The exact immunogen used to generate this antibody is proprietary information.

P21926

Epitope

The antibody recognizes an epitope on second extracellular domain (EC2) of CD9 antigen, a protein of 24 kDa belonging to tetraspans family (TM4SF).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "2-5 µg/mL", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/biotin-mouse-igg1-kappa-monoclonal-mopc-21-isotype-control-ab18434'>ab18434</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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Product details

The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD9 also known as motility-related protein-1 (MRP-1) is a transmembrane glycoprotein with a molecular weight of approximately 24 kDa. This protein is part of the tetraspanin family and shows widespread expression throughout different tissues including hematopoietic and non-hematopoietic cells. CD9 participates in numerous cellular processes by interacting with integrins and other cell surface receptors facilitating the organization of molecular complexes within the cell membrane.

Biological function summary

The CD9 protein is integral to cell adhesion migration and fusion. It forms complexes with other tetraspanins and proteins like EWI-2 and EWI-F contributing to cellular signaling and membrane compartmentalization. Additionally CD9 plays an important role in the formation and secretion of exosomes tiny vesicles emitted by cells that mediate cell-to-cell communication. These exosomes can be analyzed using tools like exosome assays and detection kits that target CD9 proteins.

Pathways

CD9 is involved in the regulation of immune response and cell morphology. It participates in pathways such as the integrin signaling pathway and the phosphatidylinositol 3-kinase (PI3K) signaling pathway. CD9 modulates these interactions by associating with proteins such as integrins and other members of the tetraspanin family influencing cellular movement and proliferation.

CD9 has connections to various conditions including cancer and infectious diseases. In cancer CD9 can influence tumor progression and metastasis while its altered expression levels have been associated with different cancer types. CD9 interacts with proteins such as integrins and CD81 in these contexts affecting cellular adhesion and migration mechanisms. In infectious diseases CD9 is involved in viral entry processes as some viruses utilize CD9 and its associated tetraspanins for entry into host cells.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion (PubMed : 14575715, PubMed : 18541721, PubMed : 8478605). Present at the cell surface of oocytes and plays a key role in sperm-egg fusion, possibly by organizing multiprotein complexes and the morphology of the membrane required for the fusion (By similarity). In myoblasts, associates with CD81 and PTGFRN and inhibits myotube fusion during muscle regeneration (By similarity). In macrophages, associates with CD81 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles (PubMed : 12796480). Also prevents the fusion between mononuclear cell progenitors into osteoclasts in charge of bone resorption (By similarity). Acts as a receptor for PSG17 (By similarity). Involved in platelet activation and aggregation (PubMed : 18541721). Regulates paranodal junction formation (By similarity). Involved in cell adhesion, cell motility and tumor metastasis (PubMed : 7511626, PubMed : 8478605).

See full target information CD9

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:17707 PubMed37853206

2023

Use of transcranial low-intensity focused ultrasound for targeted delivery of stem cell-derived exosomes to the brain.

Applications

Unspecified application

Species

Unspecified reactive species

J Haroon,K Aboody,L Flores,M McDonald,K Mahdavi,M Zielinski,K Jordan,E Rindner,J Surya,V Venkatraman,V Go-Stevens,G Ngai,J Lara,C Hyde,S Schafer,M Schafer,A Bystritsky,I Nardi,T Kuhn,D Ross,S Jordan

Advanced materials (Deerfield Beach, Fla.) 34:e2200981 PubMed35429065

2022

SEEDING to Enable Sensitive Electrochemical Detection of Biomarkers in Undiluted Biological Samples.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan Sabaté Del Río,Hyun-Kyung Woo,Juhee Park,Hong Koo Ha,Jae-Ryong Kim,Yoon-Kyoung Cho

Methods in molecular biology (Clifton, N.J.) 1619:263-301 PubMed28674892

2017

Surface Profiling of Extracellular Vesicles from Plasma or Ascites Fluid Using DotScan Antibody Microarrays.

Applications

Unspecified application

Species

Unspecified reactive species

Larissa Belov,Susannah Hallal,Kieran Matic,Jerry Zhou,Sandra Wissmueller,Nuzhat Ahmed,Sumaiya Tanjil,Stephen P Mulligan,O Giles Best,Richard J Simpson,Richard I Christopherson

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-CD9 antibody [MEM-61]

Flow Cytometry - Biotin Anti-CD9 antibody [MEM-61] (AB28094)

Flow Cytometry - Biotin Anti-CD9 antibody [MEM-61] (AB28094)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Epitope

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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