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AB322770

Biotin Anti-Complement C8a antibody [EPR23646-33] (Detector)

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Rabbit Recombinant Monoclonal CO8A antibody - conjugated to Biotin. Suitable for sELISA and reacts with Human samples.

View Alternative Names

Complement component C8 alpha chain, Complement component 8 subunit alpha, C8A

  • Carrier free

    Anti-Complement C8a antibody [EPR23646-33] - BSA and Azide free (Detector)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23646-33

Isotype

IgG

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Complement C8a also known as C8 alpha chain is a part of the complement system which plays a role in immune response. It has a molecular mass of approximately 64 kDa. This protein is found in blood plasma and is mainly expressed in hepatocytes. The C8a forms the terminal subunit of the C8 protein complex which is critical for the formation of the membrane attack complex (MAC) that disrupts the lipid bilayer of target cells.
Biological function summary

The C8a chain works in concert with C8 beta and C8 gamma to form the C8 complex of the complement system. This protein becomes part of the larger MAC a lytic complex that facilitates cell membrane disruptions thereby initiating cell lysis. The membrane attack complex plays a defensive role against pathogenic microorganisms contributing to innate immunity.

Pathways

The complement system involving C8a plays a central role in the innate immune pathway and the alternative pathway of complement activation. These pathways are essential for the protection against infections. The C5 convertase acts upstream of C8a cleaving C5 to C5b which then associates with C6 and C7 allowing C8a and its counterparts C8b and C8g to bind and initiate MAC formation. C9 is the final protein to bind completing the complex and resulting in the lysis of the target.

The deficiency or dysfunction in C8a can be associated with increased susceptibility to Neisseria infections particularly meningococcal disease. C8 deficiency is often linked with deficiencies in C5-C9 proteins as these proteins are collectively critical for functional MAC formation. Immunological responses depending on the formation of the MAC can become compromised increasing vulnerability to certain bacterial infections.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C8A inserts into the target membrane, but does not form pores by itself.
See full target information C8A
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Complementary Products

Assay Kits

AB137971

Human Complement C8 ELISA Kit

Sandwich ELISA - Human Complement C8 ELISA Kit (AB137971)

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Product promise

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For full details, please see our Terms & Conditions

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