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AB84280

Biotin Anti-CX3CL1 antibody

5

(1 Review)

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(1 Publication)

Rabbit Polyclonal CX3CL1 antibody - conjugated to Biotin. Carrier free. Suitable for sELISA, WB and reacts with Recombinant fragment - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CX3CL1.

View Alternative Names

FKN, NTT, SCYD1, A-152E5.2, CX3CL1, Fractalkine, C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, Neurotactin, Small-inducible cytokine D1

1 Images
Sandwich ELISA - Biotin Anti-CX3CL1 antibody (AB84280)
  • sELISA

Supplier Data

Sandwich ELISA - Biotin Anti-CX3CL1 antibody (AB84280)

Sandwich ELISA : To detect human Fractalkine by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of CX3CL1 Polyclonal Antibody, Biotin (ab84280) is required. This biotinylated polyclonal antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant human Fractalkine.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Excitation/Emission
Carrier free

Yes

Reacts with

Human

Applications

sELISA, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CX3CL1.

P78423

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "predicted", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant fragment - Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "0.25-1 µg/mL", "sELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.2 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Lyophilized
Reconstitution
Reconstitute in PBS, 0.1% BSA
Purification notes
ab84280 was purified by affinity chromatography and then biotinylated.It is sterile filtered.
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CX3CL1 also known as fractalkine is a chemokine protein characterized mechanically by its dual function as both a chemoattractant and adhesion molecule. It exhibits a unique membrane-bound structure and can exist in a soluble form after proteolytic cleavage. The mass of CX3CL1 is approximately 42-45 kDa. This protein is highly expressed in endothelial cells neurons and tissues with substantial vascularization. Considerable interest surrounds the potential of CX3CL1 assessment in various studies often involving applications like fractalkine ELISA or assays on mouse models.
Biological function summary

CX3CL1 is involved in the immune system and nervous system functions influencing leukocyte adhesion and migration. It is not known to be part of a large multiprotein complex but directly interacts with its fractalkine receptor CX3CR1 on the surface of immune cells. This interaction regulates communication between neurons and microglia which highlights its role in neuroinflammation. CX3CL1's expression and function suggest a significant influence over inflammatory responses and homeostatic balance within these systems.

Pathways

CX3CL1 plays a role in the MAPK and PI3K-Akt pathways vital for cell survival proliferation and migration. Within these pathways CX3CL1 influences and interacts with several proteins thereby modulating inflammatory responses and cellular migration. These pathways are essential for immune system regulation where CX3CL1 acts as a mediator in signal transduction processes that connect immune responses to cellular activities such as proliferation and survival.

CX3CL1 has associations with neurodegenerative disorders like Alzheimer's disease and inflammatory diseases such as rheumatoid arthritis. Alterations in CX3CL1 expression or its fractalkine receptor function can influence disease progression by affecting how immune cells communicate and migrate to inflamed or damaged tissues. CX3CR1 the receptor for CX3CL1 represents an important link in these connections driving research efforts to explore therapies targeting these interactions to alleviate disease symptoms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Chemokine that acts as a ligand for both CX3CR1 and integrins ITGAV : ITGB3 and ITGA4 : ITGB1 (PubMed : 12055230, PubMed : 21829356, PubMed : 23125415, PubMed : 9782118, PubMed : 9931005). The CX3CR1-CX3CL1 signaling exerts distinct functions in different tissue compartments, such as immune response, inflammation, cell adhesion and chemotaxis (PubMed : 12055230, PubMed : 9024663, PubMed : 9177350, PubMed : 9782118). Regulates leukocyte adhesion and migration processes at the endothelium (PubMed : 9024663, PubMed : 9177350). Can activate integrins in both a CX3CR1-dependent and CX3CR1-independent manner (PubMed : 23125415, PubMed : 24789099). In the presence of CX3CR1, activates integrins by binding to the classical ligand-binding site (site 1) in integrins (PubMed : 23125415, PubMed : 24789099). In the absence of CX3CR1, binds to a second site (site 2) in integrins which is distinct from site 1 and enhances the binding of other integrin ligands to site 1 (PubMed : 23125415, PubMed : 24789099).. Processed fractalkine. The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils.. Fractalkine. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells.. (Microbial infection) Mediates the cytoadherence of erythrocytes infected with parasite P.falciparum (strain 3D7) with endothelial cells by interacting with P.falciparum CBP1 and CBP2 expressed at the surface of erythrocytes (PubMed : 27653778). The adhesion prevents the elimination of infected erythrocytes by the spleen (Probable).
See full target information CX3CL1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The journal of obstetrics and gynaecology research 49:2417-2426 PubMed37464974

2023

Human Wharton's jelly-derived mesenchymal stem cells prevent pregnancy loss in a rat by JAK/STAT-mediated immunomodulation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoying Ding,Rongrong Wu,Beibei Jin,Chunyu Zhu,Yuquan Zhang,Xiaoqing Yang
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Product promise

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