AB271209

Biotin Anti-CXCL2 antibody

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Polyclonal CXCL2 antibody - conjugated to Biotin. Suitable for WB, sELISA and reacts with Recombinant full length protein - Mouse, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Mouse Cxcl2 aa 1 to C-terminus.

View Alternative Names

Mip-2, Mip2, Scyb2, Cxcl2, C-X-C motif chemokine 2, Macrophage inflammatory protein 2, MIP2

2 Images

Sandwich ELISA - Biotin Anti-CXCL2 antibody (AB271209)

sELISA

Supplier Data

Sandwich ELISA - Biotin Anti-CXCL2 antibody (AB271209)

Mouse MIP-2(CXCL2) was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271209. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mMIP-2(CXCL2) protein.

Supplier Data

Western blot - Biotin Anti-CXCL2 antibody (AB271209)

Western blot analysis of varying levels of Recombinant mouse CXCL2 protein using ab271209 at 0.2 μg/ml.

Used in conjunction with compatible secondary reagents the detection limit for recombinant CXCL2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Lane ng/lane
1 Marker
2 250
3 125
4 62.5
5 31.25
6 15.625
7 7.8
8 3.9
9 1.95
10 0.975
11 0.4875
12 0.24

All lanes:

Western blot - Biotin Anti-CXCL2 antibody (ab271209)

Predicted band size: 11 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Excitation/Emission

Carrier free

Reacts with

Mouse

Applications

sELISA, WB

applications

Immunogen

Recombinant Fragment Protein within Mouse Cxcl2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P10889

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" }, "Recombinant full length protein - Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.2 mg/mL", "WB-species-notes": "<p></p>", "sELISA-species-checked": "notRecommended", "sELISA-species-dilution-info": "", "sELISA-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Lyophilized

Reconstitution

Reconstitute in PBS, 0.1% BSA

Purification technique

Affinity purification

Storage buffer

Constituents: PBS, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The CXCL2 protein also known as macrophage inflammatory protein 2 (MIP-2) or KC in the mouse model functions as a chemokine. It weighs approximately 7.8 kDa and is part of the CXC chemokine family. CXCL2 is mainly expressed in macrophages neutrophils and certain epithelial cells. It plays an important role in mediating the migration of these cells to sites of inflammation or injury.

Biological function summary

CXCL2 attracts and activates neutrophils as part of the innate immune response. It acts independently not as part of a larger complex to provoke a chemotactic response guiding neutrophils to areas of tissue injury. CXCL2 also promotes the release of other cytokines and enzymes that contribute to inflammation. Its activity is mediated through its interaction with receptors like CXCR2 on target cell surfaces.

Pathways

CXCL2 is involved in inflammatory signaling and leukocyte migration. It holds importance in the chemokine signaling pathway which regulates leukocyte trafficking. CXCL2 interacts with proteins like CXCR2 influencing the inflammation process by activating downstream kinases and other signal transduction molecules such as mitogen-activated protein kinases (MAPKs).

CXCL2 is connected to inflammatory conditions and diseases like sepsis and inflammatory bowel disease (IBD). Elevated levels of CXCL2 can enhance the inflammatory response which is linked to the progression of these conditions. The protein is also associated with tumor necrosis factor-alpha (TNF-alpha) which amplifies inflammation and tissue damage during disease processes.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Chemotactic for human polymorphonuclear leukocytes but does not induce chemokinesis or an oxidative burst.

See full target information Cxcl2

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of clinical laboratory analysis 36:e24232 PubMed34995016

2022

A combined biomarker panel shows improved sensitivity and specificity for detection of ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Mao,Yong Tang,Ming-Jing Deng,Chun-Tao Huang,Dong Lan,Wen-Zheng Nong,Li Li,Qi Wang

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-CXCL2 antibody

Sandwich ELISA - Biotin Anti-CXCL2 antibody (AB271209)

Western blot - Biotin Anti-CXCL2 antibody (AB271209)

Key facts

Host species

Clonality

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Reconstitution

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Product promise