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AB271819

Biotin Anti-Cytokeratin 14 antibody [LL002]

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(1 Publication)

Mouse Monoclonal Cytokeratin 14 antibody - conjugated to Biotin. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Mouse Krt14.

View Alternative Names

Cytokeratin-14, Keratin-14, CK-14, K14, KRT14

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Cytokeratin 14 antibody [LL002] (AB271819)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Cytokeratin 14 antibody [LL002] (AB271819)

Formalin-fixed, paraffin-embedded human skin tissue stained for Cytokeratin 14 using ab271819 at 4 μg/ml in

immunohistochemical analysis

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Cytokeratin 14 antibody [LL002] (AB271819)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Cytokeratin 14 antibody [LL002] (AB271819)

Formalin-fixed, paraffin-embedded human skin tisue stained for Cytokeratin 14 using ab271819 at 4 μg/ml in immunohistochemical analysis

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

LL002

Isotype

IgG3

Light chain type

kappa

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Mouse Krt14. The exact immunogen used to generate this antibody is proprietary information.

Q61781

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2-4 µg/mL", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 14 also known as CK14 keratin 14 or KRT14 is a type I keratin protein with a molecular mass of approximately 50 kDa. It forms intermediate filaments in epithelial cells primarily in the basal layer of stratified epithelium and is part of the cytoskeleton. Cytokeratin 14 expression appears in tissues such as the epidermis tongue and esophagus where it contributes to the structural integrity and mechanical stability of cells. Laboratories often utilize antibodies like anti-K14 and labels such as Alexa Fluor 555 or Alexa Fluor 647 to identify cytokeratin 14 in research settings.
Biological function summary

This protein significantly contributes to the maintenance and regeneration of epithelial tissues by forming a network of filaments with keratin 5. KRT14 pairs with keratin 5 to create a keratin intermediate filament complex which plays a structural role in the resilience and elasticity of epithelial tissues. This partnership is essential for the assembly of the cytoskeleton and supports the protective barrier function of the skin and related tissues.

Pathways

Cytokeratin 14 engages in pivotal cellular processes like keratinization and wound healing. The protein is actively involved in signal transduction pathways such as the epithelial cell differentiation pathway. Within these pathways KRT14 collaborates with KRT5 and other structural proteins to regulate cell proliferation and differentiation reflecting its importance in skin health and function.

Cytokeratin 14 associates with specific genetic conditions including epidermolysis bullosa simplex (EBS) and squamous cell carcinoma (SCC). In EBS mutations in the KRT14 gene lead to skin fragility. In SCC altered expression of KRT14 and related proteins such as keratin 5 can influence tumor progression and invasion. These associations highlight the critical role of KRT14 in both normal and pathological epithelial function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
See full target information Keratin, type I cytoskeletal 14
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of immunology research 2022:3351268 PubMed35571569

2022

Echinacoside Induces Mitochondria-Mediated Pyroptosis through Raf/MEK/ERK Signaling in Non-Small Cell Lung Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Shi,Hui Cao,Zhengcheng Liu,Lei Xi,Changqing Dong
View all publications
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Product promise

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