AB195906

Biotin Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker

RabMAb
Recombinant
Lab Essentials
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(1 Publication)

Rabbit Recombinant Monoclonal Cytokeratin 17 antibody - conjugated to Biotin. Cytoskeleton marker. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

39.1, Cytokeratin-17, Keratin-17, CK-17, K17, KRT17

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (AB195906)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (AB195906)

IHC image of Cytokeratin 17 staining in a section of formalin-fixed paraffin-embedded normal human skin*.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Biocare Medical NxGen pressure cooker using retrieval settings of 110^oC for 8 minutes. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C with ab195906 at 1/100 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

578 PE

PE Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker
Unconjugated

Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker
Carrier free

Anti-Cytokeratin 17 antibody [EP1623] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1623

Isotype

IgG

Conjugation

Biotin

Excitation/Emission

Carrier free

Reacts with

Human, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 17 often called K17 or keratin 17 is a type I intermediate filament protein with a molecular weight of approximately 46 kDa. It forms part of the cytoskeleton in epithelial cells and is notably present in the basal cells of epithelial tissues including the skin hair follicles and the lining of internal organs. Due to its specific expression pattern K17 serves as a useful biomarker in various research and clinical settings.

Biological function summary

The roles of K17 extend beyond mere structural support. This protein contributes to cellular processes such as proliferation tissue repair and the regulation of immune responses. K17 often forms a complex with its partner keratin 6 enabling structural stability and resilience in epithelial cells. Its presence influences the behavior of the cells in skin and other epithelial-lined organs.

Pathways

K17 participates in key pathways related to skin biology and inflammation notably the Wnt/β-catenin and NF-κB signaling pathways. These pathways connect K17 to cellular processes like growth differentiation and inflammation. In particular keratin 6 is another important protein in these cascades closely interacting with K17 in maintenance and repair processes within epithelial tissues.

K17 has been implicated in psoriasis and certain types of cancer such as cervical and breast cancer. In psoriasis an inflammatory skin condition K17 interacts with pro-inflammatory cytokines contributing to the hyperproliferative state of the skin. Its overexpression serves as a marker in diagnosing and understanding the severity of these disorders highlighting its relationship with keratin 6 which is similarly upregulated in psoriatic lesions and certain tumors. These associations make K17 a significant focus for therapeutic targeting and diagnostic applications.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Type I keratin involved in the formation and maintenance of various skin appendages, specifically in determining shape and orientation of hair (By similarity). Required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state (By similarity). Modulates the function of TNF-alpha in the specific context of hair cycling. Regulates protein synthesis and epithelial cell growth through binding to the adapter protein SFN and by stimulating Akt/mTOR pathway (By similarity). Involved in tissue repair. May be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial 'stem cells'. Acts as a promoter of epithelial proliferation by acting a regulator of immune response in skin : promotes Th1/Th17-dominated immune environment contributing to the development of basaloid skin tumors (By similarity). May act as an autoantigen in the immunopathogenesis of psoriasis, with certain peptide regions being a major target for autoreactive T-cells and hence causing their proliferation.

See full target information KRT17

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences 20:1039-45 PubMed26941806

2016

Apolipoprotein A1 as a novel anti-implantation biomarker in polycystic ovary syndrome: A case-control study.

Applications

Unspecified application

Species

Unspecified reactive species

Fatemehsadat Amjadi,Reza Aflatoonian,Shaghayegh Haghjoo Javanmard,Bita Saifi,Mahnaz Ashrafi,Mehdi Mehdizadeh

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (AB195906)

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Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise