AB27553

Biotin Anti-Cytokeratin 18 antibody [C-04]

Name: Anti-Cytokeratin 18 Biotin antibody [C-04] (ab27553) IgG1 | Abcam
Brand: Abcam Limited
SKU: AB27553
Price: 520 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(6 Publications)

Mouse Monoclonal Cytokeratin 18 antibody - conjugated to Biotin. Suitable for WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Cell preparation containing KRT18 protein.

View Alternative Names

CYK18, PIG46, KRT18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, Keratin-18, CK-18, K18

1 Images

Supplier Data

Western blot - Biotin Anti-Cytokeratin 18 antibody [C-04] (AB27553)

Proteolytic fragment observed at approximately 25 kDa.

All lanes:

Western blot - Biotin Anti-Cytokeratin 18 antibody [C-04] (ab27553) at 2 µg/mL

Lane 1:

HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate (reducing conditions)

Lane 2:

MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate (reducing conditions)

Lane 3:

MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate (non-reducing conditions)

Lane 4:

HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate (non-reducing conditions)

Secondary

All lanes:

Conjugated Streptavidin

Predicted band size: 48 kDa

Observed band size: 46 kDa

false

Unconjugated

Anti-Cytokeratin 18 antibody [C-04]
519 FITC

FITC Anti-Cytokeratin 18 antibody [C-04]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

C-04

Isotype

IgG1

Conjugation

Biotin

Excitation/Emission

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Cell preparation containing KRT18 protein. The exact immunogen used to generate this antibody is proprietary information.

P05783

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" }, "Mammals": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Purification notes

Purity >95% by SDS-PAGE.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 18 (CK18) also known as Keratin 18 or KRT18 is a type I intermediate filament protein with a molecular weight of approximately 45 kDa. It plays a mechanical role in stabilizing epithelial cell structure and function. CK18 is expressed in single-layered epithelial tissues such as the liver intestine and pancreas. It partners with the type II keratin usually KRT8 to form a stable cytoskeletal network in these cells providing structural integrity and resilience.

Biological function summary

CK18 maintains cellular integrity and mediates resistance to mechanical and non-mechanical stresses in epithelial cells. It forms heterodimers with KRT8 participating in a complex network of filaments important for maintaining cell shape and stability. This organization plays a role in managing cell survival during stress and apoptosis helping regulate cellular functions and responses in tissues where it is expressed.

Pathways

Cytokeratin 18 is involved in the apoptosis and cellular stress response pathways. It interacts with proteins such as caspases during apoptosis facilitating cytoskeletal reorganization and cell fragmentation. CK18 in coordination with KRT8 also participates in signaling pathways that regulate cell cycle and apoptotic processes underlining its importance in cellular turnover and tissue homeostasis.

Elevated levels of CK18 fragments are often observed in cases of non-alcoholic steatohepatitis (NASH) and breast cancer. It serves as a biomarker for liver injury due to its abundance in hepatocytes and release upon cell death. In breast cancer alterations in CK18 expression can reflect disease progression where it interacts with other proteins such as vimentin indicating epithelial-to-mesenchymal transition a process that facilitates metastasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

See full target information KRT18

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Nature genetics 57:2177-2191 PubMed40858905

2025

ERG-driven prostate cancer initiation is cell-context dependent and requires KMT2A and DOT1L.

Applications

Unspecified application

Species

Unspecified reactive species

Weiran Feng,Erik Ladewig,Matthew Lange,Nazifa Salsabeel,Huiyong Zhao,Young Sun Lee,Anuradha Gopalan,Hanzhi Luo,Wenfei Kang,Ning Fan,Eric Rosiek,Elisa de Stanchina,Yu Chen,Brett S Carver,Christina S Leslie,Charles L Sawyers

Cell reports 41:111641 PubMed36384107

2022

A dietary change to a high-fat diet initiates a rapid adaptation of the intestine.

Applications

Unspecified application

Species

Unspecified reactive species

Jacob R Enriquez,Heather A McCauley,Kevin X Zhang,J Guillermo Sanchez,Gregory T Kalin,Richard A Lang,James M Wells

Oncotarget 8:93899-93911 PubMed29212197

2017

Paeoniflorin prevents postoperative peritoneal adhesion formation in an experimental rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Gao,Guangbing Wei,Yunhua Wu,Na Yao,Cancan Zhou,Kai Wang,Kang Wang,Xuejun Sun,Xuqi Li

Nature cell biology 16:942-50, 1-7 PubMed25173976

2014

Mammary stem cells have myoepithelial cell properties.

Applications

IHC-P

Species

Mouse

Michael D Prater,Valérie Petit,I Alasdair Russell,Rajshekhar R Giraddi,Mona Shehata,Suraj Menon,Reiner Schulte,Ivo Kalajzic,Nicola Rath,Michael F Olson,Daniel Metzger,Marisa M Faraldo,Marie-Ange Deugnier,Marina A Glukhova,John Stingl

PloS one 8:e51671 PubMed23300950

2013

FGFR2 promotes breast tumorigenicity through maintenance of breast tumor-initiating cells.

Applications

IHC-P

Species

Mouse

Sungeun Kim,Anna Dubrovska,Richard J Salamone,John R Walker,Kathryn B Grandinetti,Ghislain M C Bonamy,Anthony P Orth,Jimmy Elliott,Diana Graus Porta,Carlos Garcia-Echeverria,Venkateshwar A Reddy

Stem cells (Dayton, Ohio) 26:2245-56 PubMed18556507

2008

Scalable selection of hepatocyte- and hepatocyte precursor-like cells from culture of differentiating transgenically modified murine embryonic stem cells.

Applications

ICC/IF

Species

Unspecified reactive species

Irina Drobinskaya,Thomas Linn,Tomo Saric,Reinhard G Bretzel,Heribert Bohlen,Jürgen Hescheler,Eugen Kolossov

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-Cytokeratin 18 antibody [C-04]

Western blot - Biotin Anti-Cytokeratin 18 antibody [C-04] (AB27553)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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