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Mouse Monoclonal Cytokeratin 18 antibody - conjugated to Biotin. Suitable for WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Cell preparation containing Keratin, type I cytoskeletal 18 protein.

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Images

Western blot - Biotin Anti-Cytokeratin 18 antibody [DA-7] (AB27549), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Conjugation

Biotin

Storage buffer

pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Cell preparation containing Keratin, type I cytoskeletal 18 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P05783

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested

Tested
Tested

Species

Human

Dilution info

1.00000-2.00000 µg/mL

Notes

-

Associated Products

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Target data

Function

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

Alternative names

Recommended products

Mouse Monoclonal Cytokeratin 18 antibody - conjugated to Biotin. Suitable for WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Cell preparation containing Keratin, type I cytoskeletal 18 protein.

Key facts

Isotype

IgG1

Conjugation

Biotin

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Cell preparation containing Keratin, type I cytoskeletal 18 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P05783
Clone number

DA-7

Purification technique

Affinity purification Protein A

Specificity

ab27549 recognises Cytokeratin peptide 18. We have data to indicate that this antibody may not cross react with wide range of other species. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Cytokeratin 18 (CK18) also known as Keratin 18 or KRT18 is a type I intermediate filament protein with a molecular weight of approximately 45 kDa. It plays a mechanical role in stabilizing epithelial cell structure and function. CK18 is expressed in single-layered epithelial tissues such as the liver intestine and pancreas. It partners with the type II keratin usually KRT8 to form a stable cytoskeletal network in these cells providing structural integrity and resilience.

Biological function summary

CK18 maintains cellular integrity and mediates resistance to mechanical and non-mechanical stresses in epithelial cells. It forms heterodimers with KRT8 participating in a complex network of filaments important for maintaining cell shape and stability. This organization plays a role in managing cell survival during stress and apoptosis helping regulate cellular functions and responses in tissues where it is expressed.

Pathways

Cytokeratin 18 is involved in the apoptosis and cellular stress response pathways. It interacts with proteins such as caspases during apoptosis facilitating cytoskeletal reorganization and cell fragmentation. CK18 in coordination with KRT8 also participates in signaling pathways that regulate cell cycle and apoptotic processes underlining its importance in cellular turnover and tissue homeostasis.

Associated diseases and disorders

Elevated levels of CK18 fragments are often observed in cases of non-alcoholic steatohepatitis (NASH) and breast cancer. It serves as a biomarker for liver injury due to its abundance in hepatocytes and release upon cell death. In breast cancer alterations in CK18 expression can reflect disease progression where it interacts with other proteins such as vimentin indicating epithelial-to-mesenchymal transition a process that facilitates metastasis.

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1 product image

  • Western blot - Biotin Anti-Cytokeratin 18 antibody [DA-7] (ab27549), expandable thumbnail

    Western blot - Biotin Anti-Cytokeratin 18 antibody [DA-7] (ab27549)

    All lanes: Western blot - Biotin Anti-Cytokeratin 18 antibody [DA-7] (ab27549) at 2 µg/mL

    Lane 1: HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate (reducing conditions)

    Lane 3: MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate (non-reducing conditions)

    Lane 4: HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate (non-reducing conditions)

    Predicted band size: 48 kDa

    Observed band size: 46 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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