Mouse Monoclonal Cytokeratin 18 antibody - conjugated to Biotin. Suitable for WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Cell preparation containing Keratin, type I cytoskeletal 18 protein.
IgG1
Mouse
Biotin
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS
Liquid
Monoclonal
WB | |
---|---|
Human | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes - |
Select an associated product type
Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
CYK18, PIG46, CYK18, PIG46, KRT18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, Keratin-18, CK-18, K18
Mouse Monoclonal Cytokeratin 18 antibody - conjugated to Biotin. Suitable for WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Cell preparation containing Keratin, type I cytoskeletal 18 protein.
IgG1
Mouse
Biotin
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS
Liquid
Monoclonal
DA-7
Affinity purification Protein A
ab27549 recognises Cytokeratin peptide 18. We have data to indicate that this antibody may not cross react with wide range of other species. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
Blue Ice
1-2 weeks
+4°C
-20°C
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
Cytokeratin 18 (CK18) also known as Keratin 18 or KRT18 is a type I intermediate filament protein with a molecular weight of approximately 45 kDa. It plays a mechanical role in stabilizing epithelial cell structure and function. CK18 is expressed in single-layered epithelial tissues such as the liver intestine and pancreas. It partners with the type II keratin usually KRT8 to form a stable cytoskeletal network in these cells providing structural integrity and resilience.
CK18 maintains cellular integrity and mediates resistance to mechanical and non-mechanical stresses in epithelial cells. It forms heterodimers with KRT8 participating in a complex network of filaments important for maintaining cell shape and stability. This organization plays a role in managing cell survival during stress and apoptosis helping regulate cellular functions and responses in tissues where it is expressed.
Cytokeratin 18 is involved in the apoptosis and cellular stress response pathways. It interacts with proteins such as caspases during apoptosis facilitating cytoskeletal reorganization and cell fragmentation. CK18 in coordination with KRT8 also participates in signaling pathways that regulate cell cycle and apoptotic processes underlining its importance in cellular turnover and tissue homeostasis.
Elevated levels of CK18 fragments are often observed in cases of non-alcoholic steatohepatitis (NASH) and breast cancer. It serves as a biomarker for liver injury due to its abundance in hepatocytes and release upon cell death. In breast cancer alterations in CK18 expression can reflect disease progression where it interacts with other proteins such as vimentin indicating epithelial-to-mesenchymal transition a process that facilitates metastasis.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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All lanes: Western blot - Biotin Anti-Cytokeratin 18 antibody [DA-7] (ab27549) at 2 µg/mL
Lane 1: HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate (reducing conditions)
Lane 3: MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate (non-reducing conditions)
Lane 4: HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate (non-reducing conditions)
Predicted band size: 48 kDa
Observed band size: 46 kDa
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