AB27551

Biotin Anti-Cytokeratin 18 antibody [DC-10]

Name: Biotin Anti-Cytokeratin 18 antibody [DC-10] (ab27551) | Abcam
Brand: Abcam Limited
SKU: AB27551

Be the first to review this product! Submit a review

(1 Publication)

Mouse Monoclonal Cytokeratin 18 antibody - conjugated to Biotin. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing KRT18 protein.

View Alternative Names

CYK18, PIG46, KRT18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, Keratin-18, CK-18, K18

2 Images

Flow Cytometry (Intracellular) - Biotin Anti-Cytokeratin 18 antibody [DC-10] (AB27551)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Biotin Anti-Cytokeratin 18 antibody [DC-10] (AB27551)

Flow Cytometry analysis (intracellular staining) of HeLa cells labeling Cytokeratin 18 with ab27551 and streptavidin APC at 6 μg / ml concentration (red), and blank sample (black)

Supplier Data

Western blot - Biotin Anti-Cytokeratin 18 antibody [DC-10] (AB27551)

All lanes:

Western blot - Biotin Anti-Cytokeratin 18 antibody [DC-10] (ab27551) at 2 µg/mL

Lane 1:

HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate (reducing conditions)

Lanes 2 - 3:

MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate (reducing conditions)

Lane 4:

HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate (non-reducing conditions)

Predicted band size: 48 kDa

Observed band size: 46 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

DC-10

Isotype

IgG1

Conjugation

Biotin

Excitation/Emission

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

Cell preparation containing KRT18 protein. The exact immunogen used to generate this antibody is proprietary information.

P05783

Specificity

ab27551 recognises cytokeratin peptide 18 (45 kDa) in human tissue.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "2-6 µg/mL", "FlowCytIntra-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purity

IgG fraction

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 18 (CK18) also known as Keratin 18 or KRT18 is a type I intermediate filament protein with a molecular weight of approximately 45 kDa. It plays a mechanical role in stabilizing epithelial cell structure and function. CK18 is expressed in single-layered epithelial tissues such as the liver intestine and pancreas. It partners with the type II keratin usually KRT8 to form a stable cytoskeletal network in these cells providing structural integrity and resilience.

Biological function summary

CK18 maintains cellular integrity and mediates resistance to mechanical and non-mechanical stresses in epithelial cells. It forms heterodimers with KRT8 participating in a complex network of filaments important for maintaining cell shape and stability. This organization plays a role in managing cell survival during stress and apoptosis helping regulate cellular functions and responses in tissues where it is expressed.

Pathways

Cytokeratin 18 is involved in the apoptosis and cellular stress response pathways. It interacts with proteins such as caspases during apoptosis facilitating cytoskeletal reorganization and cell fragmentation. CK18 in coordination with KRT8 also participates in signaling pathways that regulate cell cycle and apoptotic processes underlining its importance in cellular turnover and tissue homeostasis.

Elevated levels of CK18 fragments are often observed in cases of non-alcoholic steatohepatitis (NASH) and breast cancer. It serves as a biomarker for liver injury due to its abundance in hepatocytes and release upon cell death. In breast cancer alterations in CK18 expression can reflect disease progression where it interacts with other proteins such as vimentin indicating epithelial-to-mesenchymal transition a process that facilitates metastasis.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Required for the formation of KRT8/KRT18 filaments that are involved in ARHGEF40-mediated actin stress fiber formation and tensional force-induced stress fiber formation and reinforcement (PubMed : 26823019). Also acts downstream of ROCK kinase activation as part of a positive feedback mechanism in response to cellular mechanical stress loading (PubMed : 26823019). Organization and orientation of KRT18 filaments are responsible for the properly elongated morphology of epithelial tubules (By similarity). Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

See full target information KRT18

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biochemical and biophysical research communication 374:181-6 PubMed18621021

2008

Quantitative multiplexed quantum dot immunohistochemistry.

Applications

Unspecified application

Species

Unspecified reactive species

E Sweeney,T H Ward,N Gray,C Womack,G Jayson,A Hughes,C Dive,R Byers

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-Cytokeratin 18 antibody [DC-10]

Flow Cytometry (Intracellular) - Biotin Anti-Cytokeratin 18 antibody [DC-10] (AB27551)

Western blot - Biotin Anti-Cytokeratin 18 antibody [DC-10] (AB27551)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise