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Mouse Monoclonal DDDDK tag (Binds to FLAG® tag sequence) antibody - conjugated to Biotin. Suitable for WB and reacts with Tag samples.


Images

Western blot - Biotin Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (AB173832), expandable thumbnail

Key facts

Isotype
IgG2b
Host species
Mouse
Conjugation
Biotin
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: 99% PBS

Form
Liquid
Clonality
Monoclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Tag
Tested

Tested
Tested

Species
Tag
Dilution info
-
Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Alternative names

DDDDK epitope tag, DDDK, DYKDDDDK, DYKDDDDK (also known as the FLAG搴 tag sequence), DYKDDDDK epitope tag, DYKDDDDK tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, FLAG搴, FLAG搴 tag, ddk

Recommended products

Mouse Monoclonal DDDDK tag (Binds to FLAG® tag sequence) antibody - conjugated to Biotin. Suitable for WB and reacts with Tag samples.

Key facts

Isotype
IgG2b
Conjugation
Biotin
Form
Liquid
Clonality
Monoclonal
Clone number
FG4R
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Do Not Freeze

Notes

FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Western blot - Biotin Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (ab173832), expandable thumbnail

    Western blot - Biotin Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (ab173832)

    Note: Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. Incubation with ab173832 was for 1 hour at room temperature. Incubation with secondary antibody was for 30 miutes at room temperature.

    All lanes: Western blot - Biotin Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (ab173832) at 1/1000 dilution

    Lane 1: E. coli lysate containing a multi-epitope tagged protein at 0.25 µg

    Lane 2: E. coli lysate containing a multi-epitope tagged protein at 0.125 µg

    Lane 3: E. coli lysate containing a multi-epitope tagged protein at 0.06 µg

    Lane 4: E. coli lysate containing a multi-epitope tagged protein at 0.03 µg

    Lane 5: E. coli lysate containing a multi-epitope tagged protein at 0.015 µg

    Secondary

    All lanes: Streptavidin-HRP at 1/20000 dilution

    Developed using the ECL technique.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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