AB20640

Biotin Anti-E. coli antibody

Name: Anti-E. coli Biotin antibody (ab20640) Rabbit polyclonal IgG | Abcam
Brand: Abcam Limited
SKU: AB20640

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(26 Publications)

Rabbit Polyclonal E. coli antibody - conjugated to Biotin. Suitable for ELISA, ICC/IF and reacts with Escherichia coli samples. Cited in 26 publications. Immunogen corresponding to Cell preparation containing E. coli protein.

View Alternative Names

Escherichia coli

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Excitation/Emission

Carrier free

Reacts with

Escherichia coli

Applications

ICC/IF, ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Many "O" and "K" antigenic serotypes of Escherichia coli. Will remove E. coli proteins from recombinant preparations. Tested specifically with the strains O18, O20, O44, O55, O111, O112, O125, O157, K12.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Escherichia coli": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

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Product details

Covalently coupled with the N-Hydroxysuccinimide ester of biotin under mild conditions to give a high degree of substitution.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Protein A chromatography.

Storage buffer

Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information E. coli

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Publications (26)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 16:1532590 PubMed40213692

2025

Gut microbiota and HMGB1/NLRP3/GSDMD inflammasome-dependent pyroptosis: mechanisms by physcion ameliorates alcoholic liver fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Bai,Hao-Lin Guo,Fei Wang,Yan-Yu Kang,Hao-Tian Zhang,Lu Dong,Yong Yang

Journal of Crohn's & colitis 17:1988-2001 PubMed37462681

2023

An Intestinal Th17 Subset is Associated with Inflammation in Crohn's Disease and Activated by Adherent-invasive Escherichia coli.

Applications

Unspecified application

Species

Unspecified reactive species

Moira Paroni,Gabriella Leccese,Valeria Ranzani,Giorgia Moschetti,Matteo Chiara,Federica Perillo,Sara Ferri,Francesca Clemente,Daniele Noviello,Francesco Simone Conforti,Stefano Ferrero,Bhavna Karnani,Roberto Bosotti,Chiara Vasco,Serena Curti,Maria Cristina Crosti,Paola Gruarin,Grazisa Rossetti,Maria Pia Conte,Maurizio Vecchi,Massimiliano Pagani,Paolo Landini,Federica Facciotti,Sergio Abrignani,Flavio Caprioli,Jens Geginat

Gut microbes 13:1972746 PubMed34530693

2021

Ursolic acid reverses liver fibrosis by inhibiting NOX4/NLRP3 inflammasome pathways and bacterial dysbiosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Nie,Qi Liu,Wang Zhang,Yipeng Wan,Chenkai Huang,Xuan Zhu

Nature communications 12:4999 PubMed34404769

2021

Listeria exploits IFITM3 to suppress antibacterial activity in phagocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Joel M J Tan,Monica E Garner,James M Regeimbal,Catherine J Greene,Jorge D Rojas Márquez,Dustin A Ammendolia,Adam R R McCluggage,Taoyingnan Li,Katherine J Wu,Marija Cemma,Philip P Ostrowski,Brian Raught,Michael S Diamond,Sergio Grinstein,Robin M Yates,Darren E Higgins,John H Brumell

Talanta 230:122291 PubMed33934763

2021

A simple magnetic-assisted microfluidic method for rapid detection and phenotypic characterization of ultralow concentrations of bacteria.

Applications

Unspecified application

Species

Unspecified reactive species

Didem Rodoplu,Cherng-Shyang Chang,Cheng-Yuan Kao,Chia-Hsien Hsu

Cells 9: PubMed32752244

2020

Probiotic Lactobacillus and Bifidobacterium Strains Counteract Adherent-Invasive (AIEC) Virulence and Hamper IL-23/Th17 Axis in Ulcerative Colitis, but Not in Crohn's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriella Leccese,Alessia Bibi,Stefano Mazza,Federica Facciotti,Flavio Caprioli,Paolo Landini,Moira Paroni

Polymers 12: PubMed32414196

2020

Anti- Functionalized Silver-Doped Carbon Nanofibers for Capture of in Microfluidic Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Soshana Smith,Michael Delaney,Margaret Frey

Analytical chemistry 91:9623-9630 PubMed31282665

2019

Rapid Bacteria Detection at Low Concentrations Using Sequential Immunomagnetic Separation and Paper-Based Isotachophoresis.

Applications

Unspecified application

Species

Unspecified reactive species

Federico Schaumburg,Cody S Carrell,Charles S Henry

Mikrochimica acta 186:296 PubMed31016400

2019

Immunoassay for pathogenic bacteria using platinum nanoparticles and a hand-held hydrogen detector as transducer. Application to the detection of Escherichia coli O157:H7.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng-Jun Bu,Kui-Yu Wang,Hua-Song Bai,Yan Leng,Chuan-Jing Ju,Cheng-Yu Wang,Wen-Sen Liu,Jia-Yu Wan

Mikrochimica acta 186:57 PubMed30617909

2019

Disposable syringe-based visual immunotest for pathogenic bacteria based on the catalase mimicking activity of platinum nanoparticle-concanavalin A hybrid nanoflowers.

Applications

Unspecified application

Species

Unspecified reactive species

Kui-Yu Wang,Sheng-Jun Bu,Chuan-Jing Ju,Ye Han,Cheng-You Ma,Wen-Sen Liu,Zhong-Yi Li,Chang-Tian Li,Jia-Yu Wan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-E. coli antibody

Key facts

Host species

Clonality

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (26)

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