Mouse Monoclonal EPCAM antibody - conjugated to Biotin. Suitable for IHC-P and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Cell preparation containing EPCAM protein.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-EpCAM antibody [VU-1D9] (AB79079), expandable thumbnail](https://content.abcam.com/products/images/biotin-epcam-antibody-vu-1d9-ab79079--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img76694.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-EpCAM antibody [VU-1D9] (AB79079)")
Publications
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- Lab on a chip 19:3168-31782019Device for whole genome sequencing single circulating tumor cells from whole blood.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRen Li,Fei Jia,Weikai Zhang,Fanghao Shi,Zhiguo Fang,Hong Zhao,Zhiyuan Hu,Zewen WeiPubMed 31455953
- Acta biomaterialia 91:258-2692019Anti-EpCAM-conjugated adeno-associated virus serotype 2 for systemic delivery of EGFR shRNA: Its retargeting and antitumor effects on OVCAR3 ovarian cancer in vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSungjin Lee et. al.PubMed 31026519
- Cell reports 27:255-268.e62019Methods for Systematic Identification of Membrane Proteins for Specific Capture of Cancer-Derived Extracellular Vesicles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMikołaj Piotr Zaborowski,Kyungheon Lee,Young Jeong Na,Alessandro Sammarco,Xuan Zhang,Marcin Iwanicki,Pike See Cheah,Hsing-Ying Lin,Max Zinter,Chung-Yu Chou,Giulia Fulci,Bakhos A Tannous,Charles Pin-Kuang Lai,Michael J Birrer,Ralph Weissleder,Hakho Lee,Xandra O BreakefieldPubMed 30943406
- Scientific reports 5:153422015A Versatile Microarray Platform for Capturing Rare Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFalko Brinkmann,Michael Hirtz,Anna Haller,Tobias M Gorges,Michael J Vellekoop,Sabine Riethdorf,Volkmar Müller,Klaus Pantel,Harald FuchsPubMed 26493176
- Analytical chemistry 86:6355-622014Efficient sample preparation from complex biological samples using a sliding lid for immobilized droplet extractions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBenjamin P Casavant et. al.PubMed 24927449
- Biomedical microdevices 13:1033-422011Purification of cell subpopulations via immiscible filtration assisted by surface tension (IFAST).Applications:Unspecified applicationReactive species:Unspecified reactive speciesScott M Berry,Lindsay N Strotman,Jessica D Kueck,Elaine T Alarid,David J BeebePubMed 21796389
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- Biotin
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 0.41% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Cell preparation containing EPCAM protein. The exact immunogen used to generate this antibody is proprietary information. Database link P16422
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Rat | Not recommended |
| Ferret | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes Incubate for 30 min at RT. Staining of formalin/paraffin tissues requires digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37°C. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Incubate for 30 min at RT. Staining of formalin/paraffin tissues requires digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37°C. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. |
Species Ferret | Dilution info - | Notes Incubate for 30 min at RT. Staining of formalin/paraffin tissues requires digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37°C. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. |
Associated Products
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4 products for Alternative Version
8 products for Alternative Product
Target data
Function
May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
Alternative names
CD326, GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1, EPCAM, Epithelial cell adhesion molecule, Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, EGP, EGP314, hEGP314
Recommended products
Mouse Monoclonal EPCAM antibody - conjugated to Biotin. Suitable for IHC-P and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Cell preparation containing EPCAM protein.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- Biotin
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 0.41% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Cell preparation containing EPCAM protein. The exact immunogen used to generate this antibody is proprietary information. Database link P16422
- Clone number
- VU-1D9
- Purification technique
- Affinity purification Protein A/G
- Specificity
We have data to indicate that this antibody may not cross react with Rat or ferret. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
ab79079 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was changed from ascites to tissue culture supernatant on 29th September 2017 and product received after this date will be from tissue culture supernatant.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EpCAM also known as epithelial cell adhesion molecule is a transmembrane glycoprotein with a molecular weight of around 40 kDa. Alternative names include CD326 and TACSTD1. This protein is expressed on the surface of most epithelial cells and plays a role in cell adhesion. It is involved in maintaining the integrity of epithelial tissues by mediating homotypic cell-cell interactions which are important for the structure and function of tissues.
- Biological function summary
EpCAM facilitates signaling pathways that impact cellular processes like proliferation and differentiation. It is not a part of a larger protein complex but interacts directly with other cellular machinery to transmit intracellular signals. EpCAM influences stem cell behavior and is important in tissue homeostasis assisting in both normal regenerative processes and wound healing.
- Pathways
EpCAM participates in regulatory mechanisms involving the Wnt/β-catenin signaling pathway which affects gene expression and cell fate decisions. Through its action EpCAM modulates components like E-cadherin which is important for cell adhesion and the maintenance of the epithelial layer. It indirectly influences pathways relating to MYC a transcription factor that regulates cell cycle progression and apoptosis.
- Associated diseases and disorders
EpCAM is associated with various types of cancer including colorectal and breast cancer. Its overexpression often correlates with aggressive cancer phenotypes and poor prognosis. Besides cancers EpCAM mutations can lead to congenital tufting enteropathy a rare condition affecting intestinal epithelial structure and function. Aberrant EpCAM expression affects β-catenin levels impacting cellular pathways linked to tumor progression and epithelial integrity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-EpCAM antibody [VU-1D9] (ab79079), expandable thumbnail](https://content.abcam.com/products/images/biotin-epcam-antibody-vu-1d9-ab79079--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img76694.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-EpCAM antibody [VU-1D9] (ab79079)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-EpCAM antibody [VU-1D9] (ab79079)
ab79079 at 1-2 μg/mL staining EpCAM in human colon carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue using peroxidase-conjugate and DAB chromogen. Note cell membrane staining of tumor cells.
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