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AB79079

Biotin Anti-EpCAM antibody [VU-1D9]

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(8 Publications)

Mouse Monoclonal EPCAM antibody - conjugated to Biotin. Suitable for IHC-P and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Cell preparation containing EPCAM protein.

View Alternative Names

CD326, GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1, EPCAM, Epithelial cell adhesion molecule, Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, EGP, EGP314, hEGP314

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-EpCAM antibody [VU-1D9] (AB79079)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-EpCAM antibody [VU-1D9] (AB79079)

ab79079 at 1-2 μg/mL staining EpCAM in human colon carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue using peroxidase-conjugate and DAB chromogen. Note cell membrane staining of tumor cells.

  • Carrier free

    Anti-EpCAM antibody [VU-1D9] - BSA and Azide free

  • Unconjugated

    Anti-EpCAM antibody [VU-1D9]

  • 519 FITC

    FITC Anti-EpCAM antibody [VU-1D9]

  • 778 PE/Cy7®

    PE/Cy7® Anti-EpCAM antibody [VU-1D9]

  • 660 APC

    APC Anti-EpCAM antibody [VU-1D9]

  • 578 PE

    PE Anti-EpCAM antibody [VU-1D9]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-EpCAM antibody [VU-1D9]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

VU-1D9

Isotype

IgG1

Light chain type

kappa

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Cell preparation containing EPCAM protein. The exact immunogen used to generate this antibody is proprietary information.

P16422

Specificity

We have data to indicate that this antibody may not cross react with Rat or ferret. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.

ab79079 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>Incubate for 30 min at RT. Staining of formalin/paraffin tissues requires digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37°C.</p> Perform enzymatic antigen retrieval before commencing with IHC staining protocol." }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>Incubate for 30 min at RT. Staining of formalin/paraffin tissues requires digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37°C.</p> Perform enzymatic antigen retrieval before commencing with IHC staining protocol." }, "Ferret": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>Incubate for 30 min at RT. Staining of formalin/paraffin tissues requires digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37°C.</p> Perform enzymatic antigen retrieval before commencing with IHC staining protocol." } } }

Product details

This product was changed from ascites to tissue culture supernatant on 29th September 2017 and product received after this date will be from tissue culture supernatant.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EpCAM also known as epithelial cell adhesion molecule is a transmembrane glycoprotein with a molecular weight of around 40 kDa. Alternative names include CD326 and TACSTD1. This protein is expressed on the surface of most epithelial cells and plays a role in cell adhesion. It is involved in maintaining the integrity of epithelial tissues by mediating homotypic cell-cell interactions which are important for the structure and function of tissues.
Biological function summary

EpCAM facilitates signaling pathways that impact cellular processes like proliferation and differentiation. It is not a part of a larger protein complex but interacts directly with other cellular machinery to transmit intracellular signals. EpCAM influences stem cell behavior and is important in tissue homeostasis assisting in both normal regenerative processes and wound healing.

Pathways

EpCAM participates in regulatory mechanisms involving the Wnt/β-catenin signaling pathway which affects gene expression and cell fate decisions. Through its action EpCAM modulates components like E-cadherin which is important for cell adhesion and the maintenance of the epithelial layer. It indirectly influences pathways relating to MYC a transcription factor that regulates cell cycle progression and apoptosis.

EpCAM is associated with various types of cancer including colorectal and breast cancer. Its overexpression often correlates with aggressive cancer phenotypes and poor prognosis. Besides cancers EpCAM mutations can lead to congenital tufting enteropathy a rare condition affecting intestinal epithelial structure and function. Aberrant EpCAM expression affects β-catenin levels impacting cellular pathways linked to tumor progression and epithelial integrity.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
See full target information EPCAM

Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Journal of extracellular vesicles 14:e70089 PubMed40545963

2025

Extracellular Vesicle Proteome Analysis Improves Diagnosis of Recurrence in Triple-Negative Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ju-Yong Hyon,Min Woo Kim,Kyung-A Hyun,Yeji Yang,Seongmin Ha,Jee Ye Kim,Young Kim,Sunyoung Park,Hogyeong Gawk,Heaji Lee,Suji Lee,Sol Moon,Eun Hee Han,Jin Young Kim,Ji Yeong Yang,Hyo-Il Jung,Seung Il Kim,Young-Ho Chung

Frontiers in molecular biosciences 12:1531108 PubMed39911266

2025

Labeling tumor-associated extracellular vesicles with antibody-DNA conjugates for quantitative analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Du,Hongxiu Li,Shiyi Shen,Chao Tian,Xiaohuan Cao,Xingang Xu,Nan Xu,Shuling Wang,Qingchang Tian

Lab on a chip 19:3168-3178 PubMed31455953

2019

Device for whole genome sequencing single circulating tumor cells from whole blood.

Applications

Unspecified application

Species

Unspecified reactive species

Ren Li,Fei Jia,Weikai Zhang,Fanghao Shi,Zhiguo Fang,Hong Zhao,Zhiyuan Hu,Zewen Wei

Acta biomaterialia 91:258-269 PubMed31026519

2019

Anti-EpCAM-conjugated adeno-associated virus serotype 2 for systemic delivery of EGFR shRNA: Its retargeting and antitumor effects on OVCAR3 ovarian cancer in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Sungjin Lee,Hyung Jun Ahn

Cell reports 27:255-268.e6 PubMed30943406

2019

Methods for Systematic Identification of Membrane Proteins for Specific Capture of Cancer-Derived Extracellular Vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Mikołaj Piotr Zaborowski,Kyungheon Lee,Young Jeong Na,Alessandro Sammarco,Xuan Zhang,Marcin Iwanicki,Pike See Cheah,Hsing-Ying Lin,Max Zinter,Chung-Yu Chou,Giulia Fulci,Bakhos A Tannous,Charles Pin-Kuang Lai,Michael J Birrer,Ralph Weissleder,Hakho Lee,Xandra O Breakefield

Scientific reports 5:15342 PubMed26493176

2015

A Versatile Microarray Platform for Capturing Rare Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Falko Brinkmann,Michael Hirtz,Anna Haller,Tobias M Gorges,Michael J Vellekoop,Sabine Riethdorf,Volkmar Müller,Klaus Pantel,Harald Fuchs

Analytical chemistry 86:6355-62 PubMed24927449

2014

Efficient sample preparation from complex biological samples using a sliding lid for immobilized droplet extractions.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin P Casavant,David J Guckenberger,David J Beebe,Scott M Berry

Biomedical microdevices 13:1033-42 PubMed21796389

2011

Purification of cell subpopulations via immiscible filtration assisted by surface tension (IFAST).

Applications

Unspecified application

Species

Unspecified reactive species

Scott M Berry,Lindsay N Strotman,Jessica D Kueck,Elaine T Alarid,David J Beebe
View all publications

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