AB15694

Biotin Anti-F4/80 antibody [BM8]

Name: Anti-F4/80 Biotin antibody [BM8] (ab15694) Rat monoclonal IgG2a | Abcam
Brand: Abcam Limited
SKU: AB15694
Price: 270 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(9 Publications)

Rat Monoclonal Adgre1 antibody - conjugated to Biotin. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 9 publications. Immunogen corresponding to Cell preparation containing Adgre1 protein.

View Alternative Names

Adgre1

1 Images

Flow Cytometry - Biotin Anti-F4/80 antibody [BM8] (AB15694)

Flow Cyt

Supplier Data

Flow Cytometry - Biotin Anti-F4/80 antibody [BM8] (AB15694)

Flow Cytometry analysis of C57Bl/6 Mouse bone marrow cells labeling APC Anti-Mouse CD11b and 0.125 μg Biotin Anti-F4/80 antibody (ab15694) (right panel) or 0.125 μg Biotin Rat IgG2b isotype control (left panel), followed by Streptavidin PE.

Unconjugated

Anti-F4/80 antibody [BM8]
519 FITC

FITC Anti-F4/80 antibody [BM8]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

BM8

Isotype

IgG2a

Conjugation

Biotin

Excitation/Emission

Carrier free

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Epitope

F4/80 antigen.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }

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Product details

The antigen is a 125kD cell surface membrane bound protein (non-reducing conditions). It is sensitive to 2 mercaptoethanol.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.14% Sodium dihydrogen phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze|Store undiluted

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

F4/80 also known as Emr1 (Epidermal growth factor module-containing mucin-like hormone receptor 1) is a well-characterized surface protein with a mass of approximately 160 kDa. This protein is recognized as a marker for murine macrophages. F4/80 is heavily expressed on cells of the monocyte and macrophage lineage particularly in tissue-resident macrophages found in organs like the liver spleen and lung. Its expression is vital for identifying macrophages in various types of immunological studies including F4/80 staining and macrophage marker analysis.

Biological function summary

F4/80 plays a role in modulating the immune response by interacting with other cell surface molecules. While not part of a larger protein complex F4/80 influences macrophage functions such as phagocytosis and cytokine production. Its expression contributes to the regulation of tissue homeostasis and inflammation. The protein is critical in maintaining the macrophage population required for reliable immune surveillance and response to pathogens.

Pathways

F4/80 is an integral part of the immunological and inflammatory signaling pathways. It interacts with TLRs (Toll-like receptors) and other cell surface molecules that sense microbial components therefore helping in the initiation of innate immune responses. F4/80 is connected to proteins like cytokine receptors which play roles in mediating and resolving inflammatory signals important for maintaining balanced immune activity.

F4/80 is associated with conditions like chronic inflammation and autoimmune disorders. Its expression levels can often correlate with disease state impacting diseases such as rheumatoid arthritis and liver fibrosis. In these conditions F4/80 interacts with proteins like TNF-alpha and IL-1beta which are involved in inflammatory processes. Understanding F4/80's role can help uncover therapeutic targets aimed at modulating macrophage functions to treat these disorders effectively.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Adgre1

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in endocrinology 13:1024769 PubMed36686492

2023

Increased plasma genistein after bariatric surgery could promote remission of NAFLD in patients with obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Geng Wang,Yu Wang,Jie Bai,Gang Li,Yang Liu,Shichang Deng,Rui Zhou,Kaixiong Tao,Zefeng Xia

Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society 28:772-779 PubMed32856346

2020

Annexin A1-derived peptide Ac2-26 facilitates wound healing in diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Jie Huang,Chong-Jian Xia,Ying Wei,Yi Yao,Miao-Wu Dong,Ke-Zhi Lin,Lin-Sheng Yu,Yuan Gao,Yan-Yan Fan

Parasites & vectors 12:457 PubMed31547847

2019

Recombinant Sj16 protein with novel activity alleviates hepatic granulomatous inflammation and fibrosis induced by Schistosoma japonicum associated with M2 macrophages in a mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Shen,Lifu Wang,Mei Peng,Zhen Liu,Beibei Zhang,Tao Zhou,Xi Sun,Zhongdao Wu

Viruses 10: PubMed30249047

2018

Mouse Gamma Herpesvirus MHV-68 Induces Severe Gastrointestinal (GI) Dilatation in Interferon Gamma Receptor-Deficient Mice (IFNγR) That Is Blocked by Interleukin-10.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Chen,Mee Yong Bartee,Jordan R Yaron,Liying Liu,Liqiang Zhang,Donghang Zheng,Ian B Hogue,Whitney L Bullard,Scott Tibbetts,Alexandra R Lucas

Scientific reports 7:43745 PubMed28262799

2017

Dietary restriction protects against diethylnitrosamine-induced hepatocellular tumorigenesis by restoring the disturbed gene expression profile.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Duan,Wenjie Sun,Mohan Zhang,Juan Ge,Yansu He,Jun Zhang,Yifan Zheng,Wei Yang,Han-Ming Shen,Jun Yang,Xinqiang Zhu,Peilin Yu

EMBO molecular medicine 9:265-279 PubMed28053183

2017

The transcription factor GATA4 promotes myocardial regeneration in neonatal mice.

Applications

Unspecified application

Species

Unspecified reactive species

Mona Malek Mohammadi,Badder Kattih,Andrea Grund,Natali Froese,Mortimer Korf-Klingebiel,Anna Gigina,Ulrike Schrameck,Carsten Rudat,Qiangrong Liang,Andreas Kispert,Kai C Wollert,Johann Bauersachs,Joerg Heineke

Bone & joint research 3:262-72 PubMed25185444

2014

T lymphocytes are not required for the development of fatty degeneration after rotator cuff tear.

Applications

IHC-Fr

Species

Rat

J Gumucio,M Flood,J Harning,A Phan,S Roche,E Lynch,A Bedi,C Mendias

PLoS pathogens 10:e1004026 PubMed24651834

2014

The role of host and microbial factors in the pathogenesis of pneumococcal bacteraemia arising from a single bacterial cell bottleneck.

Applications

Flow Cyt

Species

Mouse

Alice Gerlini,Leonarda Colomba,Leonardo Furi,Tiziana Braccini,Ana Sousa Manso,Andrea Pammolli,Bo Wang,Antonio Vivi,Maria Tassini,Nico van Rooijen,Gianni Pozzi,Susanna Ricci,Peter W Andrew,Uwe Koedel,E Richard Moxon,Marco R Oggioni

The Journal of biological chemistry 281:31950-62 PubMed16920708

2006

Mitochondrial protein nitration primes neurodegeneration in experimental autoimmune encephalomyelitis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoping Qi,Alfred S Lewin,Liang Sun,William W Hauswirth,John Guy

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-F4/80 antibody [BM8]

Flow Cytometry - Biotin Anti-F4/80 antibody [BM8] (AB15694)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Epitope

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

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