AB122882

Biotin Anti-Fc epsilon RI/FCER1A antibody [CRA1]

Name: Biotin Anti-Fc epsilon RI/FCER1A antibody [CRA1] (ab122882) | Abcam
Brand: Abcam Limited
SKU: AB122882
Price: 490 USD
Availability: InStock

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(4 Publications )

Anti-Fc epsilon RI/FCER1A antibody [CRA1] conjugated to Biotin - suitable for flow cytometry, and reacts with human samples. Clone [CRA1] is a mouse monoclonal antibody cited in 8 publications.

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Flow Cytometry - Biotin Anti-Fc epsilon RI/FCER1A antibody [CRA1] (AB122882)

Flow Cyt

Unknown

Flow Cytometry - Biotin Anti-Fc epsilon RI/FCER1A antibody [CRA1] (AB122882)

FACS analysis of CHO/alpha beta gamma cells (1x10⁵) with CRA1 and CRA2 antibodies by indirect-immunostaining, using FITC-labeled secondary antibody.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CRA1

Isotype

IgG2b

Light chain type

kappa

Conjugation

Biotin

Excitation/Emission

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human FCER1A aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

P12319

Epitope

corresponds to Amino acids 1-84 of Human Fc epsilon RI/FCER1A

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/biotin-mouse-igg2b-kappa-monoclonal-mpc-11-isotype-control-ab18418'>ab18418</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>" } } }

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Product details

[biotin]/[IgG] = 8.9

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Properties and storage information

Form

Liquid

Purification technique

Ion exchange chromatography

Purification notes

The IgG fraction was purified from serum free culture medium of mouse hybridoma (CRA1) by propriety chromatography under mild conditions.Filter sterilized.

Storage buffer

pH: 6 - 8.5 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

High-affinity receptor for immunoglobulin epsilon/IgE. Mediates IgE effector functions in myeloid cells. Upon IgE binding and antigen/allergen cross-linking initiates signaling pathways that lead to myeloid cell activation and differentiation. On mast cells, basophils and eosinophils stimulates the secretion of vasoactive amines, lipid mediators and cytokines that contribute to inflammatory response, tissue remodeling and cytotoxicity against microbes. Triggers the immediate hypersensitivity response to allergens as a host defense mechanism against helminth parasites, pathogenic bacteria and venom toxicity. When dysregulated, it can elicit harmful life-threatening allergic and anaphylactic reactions.

See full target information FCER1A

Alternative Names

FCE1A, FCER1A, High affinity immunoglobulin epsilon receptor subunit alpha, Fc-epsilon RI-alpha, IgE Fc receptor subunit alpha, FcERI

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Publications (4)

Recent publications for all applications. Explore the full listand refine your search

PloS one 5:e9023 PubMed20126404

2010

The high affinity IgE receptor Fc epsilonRI is expressed by human intestinal epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Eva Untersmayr,Giovanna Bises,Philipp Starkl,Charles L Bevins,Otto Scheiner,George Boltz-Nitulescu,Fritz Wrba,Erika Jensen-Jarolim

Bioscience, biotechnology, and biochemistry 65:79-85 PubMed11272849

2001

Direct expression of the extracellular portion of human FcepsilonRIalpha chain as inclusion bodies in Escherichia coli.

Applications

Unspecified application

Species

Unspecified reactive species

T Takai,K Okumura,C Ra

Bioscience, biotechnology, and biochemistry 64:1856-67 PubMed11055388

2000

Epitope analysis and primary structures of variable regions of anti-human FcepsilonRI monoclonal antibodies, and expression of the chimeric antibodies fused with human constant regions.

Applications

Unspecified application

Species

Unspecified reactive species

T Takai,T Yuuki,N Iwamoto-Yasue,K Okumura,C Ra

Journal of immunology (Baltimore, Md. : 1950) 162:5455-65 PubMed10228025

1999

IgE enhances Fc epsilon receptor I expression and IgE-dependent release of histamine and lipid mediators from human umbilical cord blood-derived mast cells: synergistic effect of IL-4 and IgE on human mast cell Fc epsilon receptor I expression and mediator release.

Applications

Unspecified application

Species

Unspecified reactive species

M Yamaguchi,K Sayama,K Yano,C S Lantz,N Noben-Trauth,C Ra,J J Costa,S J Galli

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-Fc epsilon RI/FCER1A antibody [CRA1]

Flow Cytometry - Biotin Anti-Fc epsilon RI/FCER1A antibody [CRA1] (AB122882)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Epitope

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Alternative Names

Publications (4)

Product promise