JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB83163

Biotin Anti-FGF1 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal FGF1 antibody - conjugated to Biotin. Carrier free. Suitable for WB, sELISA and reacts with Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human FGF1.

View Alternative Names

FGFA, FGF1, Fibroblast growth factor 1, FGF-1, Acidic fibroblast growth factor, Endothelial cell growth factor, Heparin-binding growth factor 1, aFGF, ECGF, HBGF-1

2 Images
Sandwich ELISA - Biotin Anti-FGF1 antibody (AB83163)
  • sELISA

Supplier Data

Sandwich ELISA - Biotin Anti-FGF1 antibody (AB83163)

To detect human FGF1 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab83163 is required. This biotinylated polyclonal antibody, in conjunction with an anti-FGF1 (ab9588) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant human FGF1.

Western blot - Biotin Anti-FGF1 antibody (AB83163)
  • WB

Supplier Data

Western blot - Biotin Anti-FGF1 antibody (AB83163)

To detect human FGF1 by Western Blot analysis ab83163 can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human FGF1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Image shown is non-reducing conditions; Human recombinant FGF1 protein at 250 - 0.24 ng/ml.

Lane 1 is MW marker, lanes 2-12 human recombinant FGF1 protein.

All lanes:

Western blot - Biotin Anti-FGF1 antibody (ab83163)

Predicted band size: 17 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Excitation/Emission
Carrier free

Yes

Applications

WB, sELISA

applications

Immunogen

Recombinant Full Length Protein corresponding to Human FGF1.

P05230

style
left-column

Alternative Products

Primary Antibodies

AB9588

Anti-FGF1 antibody

Sandwich ELISA - Anti-FGF1 antibody (AB9588)

  • 5
  • 2
Primary Antibodies

AB207321

Anti-FGF1 antibody [EPR19989]

RabMAb|Recombinant

Western blot - Anti-FGF1 antibody [EPR19989] (AB207321)

  • 0
  • 0

Complementary Products

Assay Kits

AB219636

Human FGF1 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human FGF1 ELISA Kit (AB219636)

  • 0
  • 0
Primary Antibodies

AB225477

Alexa Fluor® 647 Anti-FGF2 antibody [EPR20145-219]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-FGF2 antibody [EPR20145-219] (AB225477)

  • 0
  • 0
Primary Antibodies

AB242990

Anti-EGF antibody [EPR19899-46] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-EGF antibody [EPR19899-46] - BSA and Azide free (Detector) (AB242990)

  • 0
  • 0
Assay Kits

AB219636

Human FGF1 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human FGF1 ELISA Kit (AB219636)

  • 0
  • 0
Proteins & Peptides

AB287946

Recombinant Mouse FGF1 protein

SDS-PAGE - Recombinant Mouse FGF1 protein (AB287946)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.2 µg/mL", "WB-species-notes": "<p>The detection limit for recombinant human FGF acidic is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.</p>", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "0.25-1 µg/mL", "sELISA-species-notes": "<p>Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human FGF acidic.</p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Lyophilized
Reconstitution
Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification technique
Affinity purification Protein A
Purification notes
ab83163 is sterile filtered.
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FGF1 also known as acidic fibroblast growth factor (aFGF) is a member of the fibroblast growth factor family. This protein has a molecular mass of approximately 17 kDa. FGF1 is expressed in a variety of tissues such as the brain liver and heart and plays an essential role in cell growth development and repair. The FGF1 protein is sometimes studied using techniques such as FGF1 ELISA which helps in quantifying and understanding its expression levels and activity in different contexts.
Biological function summary

FGF1 is involved in numerous cellular processes beyond simple cell proliferation. It binds to fibroblast growth factor receptors (FGFRs) on the cell surface initiating a cascade of downstream signaling events. This protein does not act alone; it is often a part of larger protein complexes that modulate cellular processes such as angiogenesis and wound healing. FGF1 is also implicated in nerve cell differentiation and survival through FGFR-mediated pathways.

Pathways

The FGF1 protein is integrally involved in the MAPK/ERK signaling pathway a critical pathway that regulates cell division growth and differentiation. Through this pathway FGF1 interacts with various proteins including Ras Raf and ERK to propagate the signal necessary for mitogenic and survival responses. FGF1 is also a player in the phosphoinositide 3-kinase (PI3K) pathway which helps control cellular responses to stress and metabolic regulation showing its role in cellular homeostasis.

FGF1 is associated with conditions such as cancer and cardiovascular diseases. The deregulation of FGF1 expression or its signaling pathways can lead to tumor development and progression with FGF1 often interacting with other proteins like the basic fibroblast growth factor (bFGF) in these contexts. In cardiovascular diseases altered FGF1 signaling might contribute to atherosclerosis and cardiac repair processes where its interaction with other growth factors like vascular endothelial growth factor (VEGF) is significant.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as a potent mitogen in vitro. Acts as a ligand for FGFR1 and integrins. Binds to FGFR1 in the presence of heparin leading to FGFR1 dimerization and activation via sequential autophosphorylation on tyrosine residues which act as docking sites for interacting proteins, leading to the activation of several signaling cascades. Binds to integrin ITGAV : ITGB3. Its binding to integrin, subsequent ternary complex formation with integrin and FGFR1, and the recruitment of PTPN11 to the complex are essential for FGF1 signaling. Induces the phosphorylation and activation of FGFR1, FRS2, MAPK3/ERK1, MAPK1/ERK2 and AKT1 (PubMed : 18441324, PubMed : 20422052). Can induce angiogenesis (PubMed : 23469107).
See full target information FGF1
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

American journal of physiology. Lung cellular and 316:L757-L763 PubMed30840481

2019

Age-dependent pulmonary reactivity to house dust mite allergen: a model of adult-onset asthma?

Applications

Unspecified application

Species

Unspecified reactive species

Savannah Mack,Jinho Shin,Yoomin Ahn,Alejandro R Castaneda,Janice Peake,Ciara Fulgar,JingJing Zhang,Yoon Hee Cho,Kent E Pinkerton
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com