Rabbit Polyclonal FGF1 antibody - conjugated to Biotin. Carrier free. Suitable for WB, sELISA and reacts with Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human FGF1.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
Biotin
- Storage buffer
Constituents: PBS
- Form
Lyophilized
- Clonality
Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human FGF1. Database link P05230
Reactivity data
Select an application| WB | sELISA | |
|---|---|---|
| Recombinant full length protein - Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info 0.1-0.2 µg/mL | Notes The detection limit for recombinant human FGF acidic is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info 0.25-1 µg/mL | Notes Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human FGF acidic. |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Acts as a ligand for FGFR1 and integrins. Binds to FGFR1 in the presence of heparin leading to FGFR1 dimerization and activation via sequential autophosphorylation on tyrosine residues which act as docking sites for interacting proteins, leading to the activation of several signaling cascades. Binds to integrin ITGAV:ITGB3. Its binding to integrin, subsequent ternary complex formation with integrin and FGFR1, and the recruitment of PTPN11 to the complex are essential for FGF1 signaling. Induces the phosphorylation and activation of FGFR1, FRS2, MAPK3/ERK1, MAPK1/ERK2 and AKT1 (PubMed:18441324, PubMed:20422052). Can induce angiogenesis (PubMed:23469107).
Alternative names
Fibroblast growth factor 1, FGF-1, Acidic fibroblast growth factor, Endothelial cell growth factor, Heparin-binding growth factor 1, aFGF, ECGF, HBGF-1, FGFA, FGF1
Recommended products
Rabbit Polyclonal FGF1 antibody - conjugated to Biotin. Carrier free. Suitable for WB, sELISA and reacts with Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human FGF1.
Alternative names
Fibroblast growth factor 1, FGF-1, Acidic fibroblast growth factor, Endothelial cell growth factor, Heparin-binding growth factor 1, aFGF, ECGF, HBGF-1, FGFA, FGF1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
Biotin
- Storage buffer
Constituents: PBS
- Form
Lyophilized
- Clonality
Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human FGF1. Database link P05230
- Carrier free
Yes
- Purification technique
Affinity purification Protein A
- Reconstitution
Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
- Concentration
- Purification notes
ab83163 is sterile filtered.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- Activity summary
FGF1 also known as acidic fibroblast growth factor (aFGF) is a member of the fibroblast growth factor family. This protein has a molecular mass of approximately 17 kDa. FGF1 is expressed in a variety of tissues such as the brain liver and heart and plays an essential role in cell growth development and repair. The FGF1 protein is sometimes studied using techniques such as FGF1 ELISA which helps in quantifying and understanding its expression levels and activity in different contexts.
- Biological function summary
FGF1 is involved in numerous cellular processes beyond simple cell proliferation. It binds to fibroblast growth factor receptors (FGFRs) on the cell surface initiating a cascade of downstream signaling events. This protein does not act alone; it is often a part of larger protein complexes that modulate cellular processes such as angiogenesis and wound healing. FGF1 is also implicated in nerve cell differentiation and survival through FGFR-mediated pathways.
- Pathways
The FGF1 protein is integrally involved in the MAPK/ERK signaling pathway a critical pathway that regulates cell division growth and differentiation. Through this pathway FGF1 interacts with various proteins including Ras Raf and ERK to propagate the signal necessary for mitogenic and survival responses. FGF1 is also a player in the phosphoinositide 3-kinase (PI3K) pathway which helps control cellular responses to stress and metabolic regulation showing its role in cellular homeostasis.
- Associated diseases and disorders
FGF1 is associated with conditions such as cancer and cardiovascular diseases. The deregulation of FGF1 expression or its signaling pathways can lead to tumor development and progression with FGF1 often interacting with other proteins like the basic fibroblast growth factor (bFGF) in these contexts. In cardiovascular diseases altered FGF1 signaling might contribute to atherosclerosis and cardiac repair processes where its interaction with other growth factors like vascular endothelial growth factor (VEGF) is significant.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Biotin Anti-FGF1 antibody (ab83163)
To detect human FGF1 by Western Blot analysis ab83163 can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human FGF1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Image shown is non-reducing conditions; Human recombinant FGF1 protein at 250 - 0.24 ng/ml.
Lane 1 is MW marker, lanes 2-12 human recombinant FGF1 protein.
All lanes: Western blot - Biotin Anti-FGF1 antibody (ab83163)
Predicted band size: 17 kDa
Sandwich ELISA - Biotin Anti-FGF1 antibody (ab83163)
To detect human FGF1 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab83163 is required. This biotinylated polyclonal antibody, in conjunction with an anti-FGF1 (Anti-FGF1 antibody ab9588) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant human FGF1.
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