Rabbit Polyclonal Fibronectin antibody - conjugated to Biotin. Suitable for WB, IHC-P and reacts with Purified native protein - Human, Human samples. Cited in 43 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human FN1.
Images
Publications
Filter by
- Materials today. Bio 23:1008422023Fibroblasts alter the physical properties of dermal ECM-derived hydrogels to create a pro-angiogenic microenvironment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeng Zhang,Fenghua Zhao,Xue Zhang,Linda A Brouwer,Janette K Burgess,Martin C HarmsenPubMed 37942422
- Industrial & engineering chemistry research 60:17417-174282021Photoinduced Gelatin-Methacrylate Scaffolds to Examine the Impact of Extracellular Environment on Trabecular Meshwork Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBikram Adhikari,Benjamin S Stinson,Matthew J Osmond,Mina B Pantcheva,Melissa D KrebsPubMed 36909833
- Advances in wound care 11:43-552021Biophysical Characterization of a Novel Tri-Layer Placental Allograft Membrane.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPaul P Bonvallet,Sita M Damaraju,Heli N Modi,Victoria L Stefanelli,Qiaoling Lin,Sunil Saini,Ankur GandhiPubMed 33975444
- Investigative ophthalmology & visual science 61:162020Dexamethasone and Glucocorticoid-Induced Matrix Temporally Modulate Key Integrins, Caveolins, Contractility, and Stiffness in Human Trabecular Meshwork Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFelix Yemanyi,Hasna Baidouri,Alan R Burns,VijayKrishna RaghunathanPubMed 33170205
- Scientific reports 10:156412020Glucocorticoid-induced cell-derived matrix modulates transforming growth factor β2 signaling in human trabecular meshwork cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFelix Yemanyi et. al.PubMed 32973273
- Biotechnology and bioengineering 117:3150-31592020Human trabecular meshwork cell behavior is influenced by collagen scaffold pore architecture and glycosaminoglycan composition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatthew J Osmond,Melissa D Krebs,Mina B PantchevaPubMed 32589791
- Aging 12:11200-112232020Endothelin-1 induces cellular senescence and fibrosis in cultured myoblasts. A potential mechanism of aging-related sarcopenia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElena Alcalde-Estévez et. al.PubMed 32572011
- Journal of biomedical materials research. Part B, Applied biomaterials 108:3076-30832020Biological characterization of dehydrated amniotic membrane allograft: Mechanisms of action and implications for wound care.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarc C Moore,Paul P Bonvallet,Sita M Damaraju,Heli N Modi,Ankur Gandhi,Peter S McFetridgePubMed 32458591
- Cureus 12:e77492020Teaching an Old Drug New Tricks: Dexamethasone as an In Vivo Inhibitor of Glioblastoma Dispersal.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArthur Carminucci,Rut Tejero,Yong Huang,Shabbar Danish,Roland H Friedel,Ramsey FotyPubMed 32455066
- Science advances 6:eaay37042020Phagocytosis of Wnt inhibitor SFRP4 by late wound macrophages drives chronic Wnt activity for fibrotic skin healing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDenise Gay et. al.PubMed 32219160
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- Biotin
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Native Full Length Protein corresponding to Human FN1. Database link P02751
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Expected | Tested |
| Mammals | Predicted | Predicted |
| Purified native protein - Human | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Purified native protein - Human | Dilution info 1/500 - 1/5000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mammals | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mammals | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Purified native protein - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Product
Target data
Function
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (PubMed:3024962, PubMed:3593230, PubMed:3900070, PubMed:7989369). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (PubMed:3024962, PubMed:3593230, PubMed:3900070, PubMed:7989369). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation (PubMed:34089617). Anastellin. Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5:ITGB1 integrin receptor signaling.
Alternative names
FN, FN1, Fibronectin, Cold-insoluble globulin, CIG
Recommended products
Rabbit Polyclonal Fibronectin antibody - conjugated to Biotin. Suitable for WB, IHC-P and reacts with Purified native protein - Human, Human samples. Cited in 43 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human FN1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- Biotin
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Native Full Length Protein corresponding to Human FN1. Database link P02751
- Purification technique
- Affinity purification
- Specificity
Typically less than 1% cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with human Fibronectin and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Laminin. Non-specific cross-reaction of anti-Fibronectin antibodies with other human serum proteins or non-Fibronectin extracellular matrix proteins is negligible.
- Concentration
- Purification notes
This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against human serum proteins and collagen and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Biotin Anti-Fibronectin antibody (ab6584)
All lanes: Western blot - Biotin Anti-Fibronectin antibody (ab6584) at 1 µg/mL
All lanes: Western blot - Native Human Fibronectin protein (Native Human Fibronectin protein ab81743) at 0.001 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 262 kDa
Exposure time: 2min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Fibronectin antibody (ab6584)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human kidney tissue sections at pH 6.0, labelling Fibronectin with ab6584 at a concentration of 10 μg/mL for 1 hour at room temperature. The secondary used was a rabbit peroxidase conjugate at a 1/10,000 dilution, incubated for 45 minutes at room temperature. Fibronectin is shown as a brown hue against a purple counterstain labelling nuclear DNA. The negative control on right (B) is without the primary antibody. Magnifications are as shown.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Fibronectin antibody (ab6584)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human kidney tissue sections at pH 9.0, labelling Fibronectin with ab6584 at a concentration of 10 μg/mL for 1 hour at room temperature. The secondary used was a rabbit peroxidase conjugate at a 1/10,000 dilution, incubated for 45 minutes at room temperature. Fibronectin is shown as a brown hue against a purple counterstain labelling nuclear DNA. The negative control on right (B) is without the primary antibody. Magnifications are as shown.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com