AB6584

Biotin Anti-Fibronectin antibody

Name: Biotin Anti-Fibronectin antibody (ab6584) | Abcam
Brand: Abcam Limited
SKU: AB6584
Price: 545 USD
Availability: InStock

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(51 Publications)

Rabbit Polyclonal Fibronectin antibody - conjugated to Biotin. Suitable for WB, IHC-P and reacts with Purified native protein - Human, Human samples. Cited in 51 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human FN1.

View Alternative Names

FN, FN1, Fibronectin, Cold-insoluble globulin, CIG

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Fibronectin antibody (AB6584)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Fibronectin antibody (AB6584)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human kidney tissue sections at pH 9.0, labelling Fibronectin with ab6584 at a concentration of 10 μg/mL for 1 hour at room temperature. The secondary used was a rabbit peroxidase conjugate at a 1/10,000 dilution, incubated for 45 minutes at room temperature. Fibronectin is shown as a brown hue against a purple counterstain labelling nuclear DNA. The negative control on right (B) is without the primary antibody. Magnifications are as shown.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Fibronectin antibody (AB6584)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human kidney tissue sections at pH 6.0, labelling Fibronectin with ab6584 at a concentration of 10 μg/mL for 1 hour at room temperature. The secondary used was a rabbit peroxidase conjugate at a 1/10,000 dilution, incubated for 45 minutes at room temperature. Fibronectin is shown as a brown hue against a purple counterstain labelling nuclear DNA. The negative control on right (B) is without the primary antibody. Magnifications are as shown.

Unknown

Western blot - Biotin Anti-Fibronectin antibody (AB6584)

All lanes:

Western blot - Biotin Anti-Fibronectin antibody (ab6584) at 1 µg/mL

All lanes:

Western blot - Native Human Fibronectin protein (<a href='/en-us/products/proteins-peptides/native-human-fibronectin-protein-ab81743'>ab81743</a>) at 0.001 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 262 kDa

true

Exposure time: 2min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Excitation/Emission

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Native Full Length Protein corresponding to Human FN1.

P02751

Specificity

Typically less than 1% cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with human Fibronectin and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Laminin. Non-specific cross-reaction of anti-Fibronectin antibodies with other human serum proteins or non-Fibronectin extracellular matrix proteins is negligible.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mammals": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Purified native protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against human serum proteins and collagen and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards.

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (PubMed : 3024962, PubMed : 3593230, PubMed : 3900070, PubMed : 7989369). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (PubMed : 3024962, PubMed : 3593230, PubMed : 3900070, PubMed : 7989369). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation (PubMed : 34089617).. Anastellin. Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.. Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5 : ITGB1 integrin receptor signaling.

See full target information FN1

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Publications (51)

Recent publications for all applications. Explore the full list and refine your search

ACS applied materials & interfaces 16:29930-29945 PubMed38819955

2024

Physical Properties and Biochemical Composition of Extracellular Matrix-Derived Hydrogels Dictate Vascularization Potential in an Organ-Dependent Fashion.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Zhang,Fenghua Zhao,Yuxuan Zhu,Linda A Brouwer,Hasse Van der Veen,Janette K Burgess,Martin C Harmsen

Nature communications 15:3784 PubMed38710716

2024

Targeted delivery of the probiotic Saccharomyces boulardii to the extracellular matrix enhances gut residence time and recovery in murine colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Mairead K Heavey,Anthony Hazelton,Yuyan Wang,Mitzy Garner,Aaron C Anselmo,Janelle C Arthur,Juliane Nguyen

Materials today. Bio 23:100842 PubMed37942422

2023

Fibroblasts alter the physical properties of dermal ECM-derived hydrogels to create a pro-angiogenic microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Zhang,Fenghua Zhao,Xue Zhang,Linda A Brouwer,Janette K Burgess,Martin C Harmsen

The Journal of allergy and clinical immunology 153:435-446.e4 PubMed37805024

2023

Airway wall extracellular matrix changes induced by bronchial thermoplasty in severe asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Pieta C Wijsman,Annika W M Goorsenberg,Noa Keijzer,Julia N S d'Hooghe,Nick H T Ten Hacken,Pallav L Shah,Els J M Weersink,Jôse Mara de Brito,Natalia de Souza Xavier Costa,Thais Mauad,Martijn C Nawijn,Judith M Vonk,Jouke T Annema,Janette K Burgess,Peter I Bonta

Experimental eye research 234:109605 PubMed37506755

2023

Characterization of extracellular matrix deposited by segmental trabecular meshwork cells.

Applications

Unspecified application

Species

Unspecified reactive species

VijayKrishna Raghunathan,Andrews Nartey,Kamesh Dhamodaran,Hasna Baidouri,Julia A Staverosky,Kate E Keller,Keith Zientek,Ashok Reddy,Ted Acott,Janice A Vranka

The Journal of clinical investigation 132: PubMed36453543

2022

Thrombospondin 1 missense alleles induce extracellular matrix protein aggregation and TM dysfunction in congenital glaucoma.

Applications

Unspecified application

Species

Unspecified reactive species

Haojie Fu,Owen M Siggs,Lachlan Sw Knight,Sandra E Staffieri,Jonathan B Ruddle,Amy E Birsner,Edward Ryan Collantes,Jamie E Craig,Janey L Wiggs,Robert J D'Amato

Experimental eye research 226:109303 PubMed36343671

2022

Cell derived matrices from bovine corneal endothelial cells as a model to study cellular dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Iman Jalilian,Santoshi Muppala,Maryam Ali,Johnathon D Anderson,Brett Phinney,Michelle Salemi,Phillip A Wilmarth,Christopher J Murphy,Sara M Thomasy,VijayKrishna Raghunathan

Industrial & engineering chemistry research 60:17417-17428 PubMed36909833

2021

Photoinduced Gelatin-Methacrylate Scaffolds to Examine the Impact of Extracellular Environment on Trabecular Meshwork Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Bikram Adhikari,Benjamin S Stinson,Matthew J Osmond,Mina B Pantcheva,Melissa D Krebs

Antioxidants (Basel, Switzerland) 10: PubMed34439556

2021

Hyperphosphatemia-Induced Oxidant/Antioxidant Imbalance Impairs Vascular Relaxation and Induces Inflammation and Fibrosis in Old Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Asenjo-Bueno,Elena Alcalde-Estévez,Mariam El Assar,Gemma Olmos,Patricia Plaza,Patricia Sosa,Patricia Martínez-Miguel,María Piedad Ruiz-Torres,Susana López-Ongil

Advances in wound care 11:43-55 PubMed33975444

2021

Biophysical Characterization of a Novel Tri-Layer Placental Allograft Membrane.

Applications

Unspecified application

Species

Unspecified reactive species

Paul P Bonvallet,Sita M Damaraju,Heli N Modi,Victoria L Stefanelli,Qiaoling Lin,Sunil Saini,Ankur Gandhi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-Fibronectin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Fibronectin antibody (AB6584)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Fibronectin antibody (AB6584)

Western blot - Biotin Anti-Fibronectin antibody (AB6584)

Key facts

Host species

Clonality

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (51)

Product promise