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AB6655

Biotin Anti-Fluorescein antibody

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(5 Publications)

Goat Polyclonal Fluorescein antibody - conjugated to Biotin. Suitable for WB, Dot, ELISA, IHC-P, IHC-Fr, IM, ISH and reacts with Chemical samples. Cited in 5 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to Fluorescein.

View Alternative Names

FI, fluorescein isothyocianate, fluorescyl

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Fluorescein antibody (AB6655)
  • IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Fluorescein antibody (AB6655)

Immunohistochemical analysis of chicken embryo neuroepithelium labelling fluorescein-tagged morpholinos with ab6655. Samples were fixed in 4% paraformaldehyde at 4°C for 1 hour.

Cropped image from Long et al, Nature Communications, 7, 10354, Fig. 7a.; doi: 10.1038/ncomms10354 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Western blot - Biotin Anti-Fluorescein antibody (AB6655)
  • WB

Supplier Data

Western blot - Biotin Anti-Fluorescein antibody (AB6655)

All lanes:

Western blot - Biotin Anti-Fluorescein antibody (ab6655) at 1/1000 dilution

All lanes:

BSA conjugated Fluorescein.

Secondary

All lanes:

Peroxidase conjugated Streptavidin secondary antibody at 1/40000 dilution

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Applications

IM, ISH, IHC-P, Dot, ELISA, IHC-Fr, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IM" : {"fullname" : "Immunomicroscopy", "shortname":"IM"}, "ISH" : {"fullname" : "In Situ Hybridisation", "shortname":"ISH"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/10000 - 1/50000", "ELISA-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/5000", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "IM-species-checked": "guaranteed", "IM-species-dilution-info": "", "IM-species-notes": "<p></p>", "ISH-species-checked": "guaranteed", "ISH-species-dilution-info": "", "ISH-species-notes": "<p></p>" } } }
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Product details

Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.

Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per goat IgG molecule.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Fluorescein Antibody Biotin Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein coupled to sepharose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage buffer
Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Fluorescein
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature 610:356-365 PubMed36198802

2022

Opposing roles of hepatic stellate cell subpopulations in hepatocarcinogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Aveline Filliol,Yoshinobu Saito,Ajay Nair,Dianne H Dapito,Le-Xing Yu,Aashreya Ravichandra,Sonakshi Bhattacharjee,Silvia Affo,Naoto Fujiwara,Hua Su,Qiuyan Sun,Thomas M Savage,John R Wilson-Kanamori,Jorge M Caviglia,LiKang Chin,Dongning Chen,Xiaobo Wang,Stefano Caruso,Jin Ku Kang,Amit Dipak Amin,Sebastian Wallace,Ross Dobie,Deqi Yin,Oscar M Rodriguez-Fiallos,Chuan Yin,Adam Mehal,Benjamin Izar,Richard A Friedman,Rebecca G Wells,Utpal B Pajvani,Yujin Hoshida,Helen E Remotti,Nicholas Arpaia,Jessica Zucman-Rossi,Michael Karin,Neil C Henderson,Ira Tabas,Robert F Schwabe

Nature 595:114-119 PubMed33915568

2021

A molecular single-cell lung atlas of lethal COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Johannes C Melms,Jana Biermann,Huachao Huang,Yiping Wang,Ajay Nair,Somnath Tagore,Igor Katsyv,André F Rendeiro,Amit Dipak Amin,Denis Schapiro,Chris J Frangieh,Adrienne M Luoma,Aveline Filliol,Yinshan Fang,Hiranmayi Ravichandran,Mariano G Clausi,George A Alba,Meri Rogava,Sean W Chen,Patricia Ho,Daniel T Montoro,Adam E Kornberg,Arnold S Han,Mathieu F Bakhoum,Niroshana Anandasabapathy,Mayte Suárez-Fariñas,Samuel F Bakhoum,Yaron Bram,Alain Borczuk,Xinzheng V Guo,Jay H Lefkowitch,Charles Marboe,Stephen M Lagana,Armando Del Portillo,Emily J Tsai,Emmanuel Zorn,Glen S Markowitz,Robert F Schwabe,Robert E Schwartz,Olivier Elemento,Anjali Saqi,Hanina Hibshoosh,Jianwen Que,Benjamin Izar

Cell reports 30:714-724.e5 PubMed31968248

2020

Remodeling of Metastatic Vasculature Reduces Lung Colonization and Sensitizes Overt Metastases to Immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Bo He,Anna Johansson-Percival,Joseph Backhouse,Ji Li,Gabriel Yin Foo Lee,Juliana Hamzah,Ruth Ganss

The Journal of pathology 245:209-221 PubMed29603739

2018

Vascular targeting of LIGHT normalizes blood vessels in primary brain cancer and induces intratumoural high endothelial venules.

Applications

Unspecified application

Species

Unspecified reactive species

Bo He,Arnaud Jabouille,Veronica Steri,Anna Johansson-Percival,Iacovos P Michael,Venkata Ramana Kotamraju,Reimar Junckerstorff,Anna K Nowak,Juliana Hamzah,Gabriel Lee,Gabriele Bergers,Ruth Ganss

Nature communications 7:10354 PubMed26838601

2016

Integrin signalling regulates the expansion of neuroepithelial progenitors and neurogenesis via Wnt7a and Decorin.

Applications

IHC-FrFl, IHC-Fr

Species

Unspecified reactive species, Unspecified reactive species

K Long,L Moss,L Laursen,L Boulter,C Ffrench-Constant
View all publications
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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