Goat Polyclonal Fluorescein antibody - conjugated to Biotin. Suitable for WB, Dot, ELISA, IHC-P, IHC-Fr, IM, ISH and reacts with Chemical samples. Cited in 4 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to Fluorescein.
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Publications
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- Nature 610:356-3652022Opposing roles of hepatic stellate cell subpopulations in hepatocarcinogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAveline Filliol,Yoshinobu Saito,Ajay Nair,Dianne H Dapito,Le-Xing Yu,Aashreya Ravichandra,Sonakshi Bhattacharjee,Silvia Affo,Naoto Fujiwara,Hua Su,Qiuyan Sun,Thomas M Savage,John R Wilson-Kanamori,Jorge M Caviglia,LiKang Chin,Dongning Chen,Xiaobo Wang,Stefano Caruso,Jin Ku Kang,Amit Dipak Amin,Sebastian Wallace,Ross Dobie,Deqi Yin,Oscar M Rodriguez-Fiallos,Chuan Yin,Adam Mehal,Benjamin Izar,Richard A Friedman,Rebecca G Wells,Utpal B Pajvani,Yujin Hoshida,Helen E Remotti,Nicholas Arpaia,Jessica Zucman-Rossi,Michael Karin,Neil C Henderson,Ira Tabas,Robert F SchwabePubMed 36198802
- Nature 595:114-1192021A molecular single-cell lung atlas of lethal COVID-19.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJohannes C Melms,Jana Biermann,Huachao Huang,Yiping Wang,Ajay Nair,Somnath Tagore,Igor Katsyv,André F Rendeiro,Amit Dipak Amin,Denis Schapiro,Chris J Frangieh,Adrienne M Luoma,Aveline Filliol,Yinshan Fang,Hiranmayi Ravichandran,Mariano G Clausi,George A Alba,Meri Rogava,Sean W Chen,Patricia Ho,Daniel T Montoro,Adam E Kornberg,Arnold S Han,Mathieu F Bakhoum,Niroshana Anandasabapathy,Mayte Suárez-Fariñas,Samuel F Bakhoum,Yaron Bram,Alain Borczuk,Xinzheng V Guo,Jay H Lefkowitch,Charles Marboe,Stephen M Lagana,Armando Del Portillo,Emily J Tsai,Emmanuel Zorn,Glen S Markowitz,Robert F Schwabe,Robert E Schwartz,Olivier Elemento,Anjali Saqi,Hanina Hibshoosh,Jianwen Que,Benjamin IzarPubMed 33915568
- The Journal of pathology 245:209-2212018Vascular targeting of LIGHT normalizes blood vessels in primary brain cancer and induces intratumoural high endothelial venules.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBo He et. al.PubMed 29603739
- Nature communications 7:103542016Integrin signalling regulates the expansion of neuroepithelial progenitors and neurogenesis via Wnt7a and Decorin.Applications:IHC-FrFl, IHC-FrReactive species:Unspecified reactive speciesK Long et. al.PubMed 26838601
Key facts
- Isotype
IgG
- Host species
Goat
- Conjugation
Biotin
- Storage buffer
pH: 6.5
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.87% Sodium chloride, 0.42% Tripotassium orthophosphate- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Chemical / Small Molecule corresponding to Fluorescein.
Reactivity data
Select an application| WB | Dot | ELISA | IHC-P | IHC-Fr | IM | ISH | |
|---|---|---|---|---|---|---|---|
| Chemical | Tested | Expected | Expected | Tested | Expected | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info 1/2000 - 1/10000 | Notes - |
Dot
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info Use at an assay dependent concentration. | Notes - |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info 1/10000 - 1/50000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info 1/1000 - 1/5000 | Notes - |
IHC-Fr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info Use at an assay dependent concentration. | Notes - |
IM
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info Use at an assay dependent concentration. | Notes - |
ISH
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Product
Target data
Alternative names
FI, fluorescein isothyocianate, fluorescyl
Recommended products
Goat Polyclonal Fluorescein antibody - conjugated to Biotin. Suitable for WB, Dot, ELISA, IHC-P, IHC-Fr, IM, ISH and reacts with Chemical samples. Cited in 4 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to Fluorescein.
Alternative names
FI, fluorescein isothyocianate, fluorescyl
Key facts
- Isotype
IgG
- Host species
Goat
- Conjugation
Biotin
- Storage buffer
pH: 6.5
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.87% Sodium chloride, 0.42% Tripotassium orthophosphate- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Chemical / Small Molecule corresponding to Fluorescein.
- Purification technique
Affinity purification
- Concentration
- Purification notes
Fluorescein Antibody Biotin Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein coupled to sepharose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Storage information
Avoid freeze / thaw cycle, Store in the dark
Notes
Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per goat IgG molecule.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Biotin Anti-Fluorescein antibody (ab6655)
All lanes: Western blot - Biotin Anti-Fluorescein antibody (ab6655) at 1/1000 dilution
All lanes: BSA conjugated Fluorescein.
SecondaryAll lanes: Peroxidase conjugated Streptavidin secondary antibody at 1/40000 dilution
Cropped image from Long et al, Nature Communications, 7, 10354, Fig. 7a.; doi: 10.1038/ncomms10354 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Fluorescein antibody (ab6655)
Immunohistochemical analysis of chicken embryo neuroepithelium labelling fluorescein-tagged morpholinos with ab6655. Samples were fixed in 4% paraformaldehyde at 4°C for 1 hour.
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